The Synthesis of a New Biofilm Containing Nano-Starch‚ Nano-Cellulose and Garlic Extract, and Assessment of Its Antimicrobial Activity for Use in Food Packaging

Subject Areas : Microbiology

F. Hashamdar Ravari ¹ , S. A. Yasini Ardakani ²

1 - دانش‌آموخته کارشناسی ارشد گروه علوم و صنایع غذایی، دانشکده کشاورزی و منابع طبیعی، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران
2 - دانشیار گروه علوم و صنایع غذایی، دانشکده کشاورزی و منابع طبیعی، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران

Received: 2017-12-04 Accepted : 2017-12-04 Published : 2017-12-22

Keywords: Allicin, Biofilm, Cellulose Nanoparticle, Milk, Polymer, Starch Nanoparticle,

Abstract :

Introduction: Antimicrobial packaging is a new trend among various active packaging strategies with greater research focus in recent years. The concomitant use of nanoparticles and antimicrobial materials in food packaging is a new approach. Therefore, this study aimed at making a biofilm based on nanocomposites containing starch and cellulose nanoparticles in addition to garlic extract, which combines degradability and antimicrobial activity. Materials and Methods: Garlic extract was extracted from fresh raw garlic and nanoparticles of cellulose and starch were synthesized biochemically. Scanning electron microscopy was used for characterization of the nanoparticles of cellulose and starch. By adding a mixture of three above materials to polyethylene polymer, a biofilm was prepared and its antimicrobial activity against two species of bacteria, yeast and mold was evaluated alone and in the presence of milk up to 6 weeks. Data were compared using student t-test. A P value of Results: The biofilm showed its greatest antimicrobial effects on E. coli (With an inhibition diameter of approximately 8.5 cm around the biofilm). Antimicrobial properties of biofilm in the presence of contaminated milk (As the packaging model of milk) were significantly higher in comparison to the control without any biofilm. Conclusion: It is possible to have a suitable antimicrobial biofilm by using nanoparticles of starch, cellulose and garlic extract. The findings of the current study suggest a new approach for food packaging industries toward the use of biodegradable active packagings which ultimately lead to quality improvement, food safety and decrease in the amount of waste.

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