The Effect of Essential Oil of Thyme (Zataria multiflora boiss) on the Sensory Properties and Oxidative Stability of Mayonnaise

Subject Areas : Microbiology

H. Khajavi ¹ (MSc Graduated of the Department of Food Science and Technology, Ardestan Branch, Islamic Azad University, Ardestan, Iran.)
A. Ahmadi Dastgerdi ² (Assistant Professor of the Department of Food Science and Technology, Ardestan Branch, Islamic Azad University, Ardestan, Iran.)

Received: 2021-01-12 Accepted : 2021-01-12 Published : 2020-12-21

Keywords: Antioxidant Activity, Essential Oil, Mayonnaise, Thyme (Zataria multifora boiss,

Abstract :

Introduction: Nowadays essential oils are considered as substitutes for synthetic additives in food products. Since the lipid oxidation is the main chemical process affecting mayonnaise deterioration, in this research, the antioxidant activity of essential oil ofthyme(Zataria multiflora boiss)was determined for oxidative stability of treated mayonnaise (homogenized) during 6 months of storage. Materials and Methods: The antioxidant activities of the essential oil of thyme(0-150 µg/g) were investigated by DPPH method. The efficiency of this essential oil (144.4 µg/g) as a natural antioxidant in mayonnaise was studied by peroxide, anisidine, totox and thiobarbituric acid values. Results: GC analysis of the essential oil resulted in the identification of forty compounds. The essential oil characterized by a high number of monoterpenes such as thymol and carvacrol. Regarding anti-oxidation, the investigated essential oil strongly reduced the DPPH radical (IC50=144.4 g/ml). This study confirms that the essential oil of thymepossessed antioxidant properties in vitro. The results showed that the treatments containing essential oil and TBHQ significantly reduced the oxidation (p<0.05), while the control sample was oxidized faster. The essential oil had a significant effect on taste, odor and overall acceptance, but no significant difference was observed in color and texture. Conclusion: The results of the present experiments suggest that essential oil of thyme(Zataria multiflora boiss)can be used as a source of natural antioxidant for the application in food industries to prevent lipid oxidation particularly lipid-containing foods such as mayonnaise. Therefore, it can use as a natural antioxidant and flavoring compound in foods such as mayonnaise.

References:

Aeschbach, R., Loe liger, J., Scott, B.C., Murcia, A., Butler, J., Halliwel, B. & Aruoma, O. I. (1994). Antioxidant action of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food Chemistry Toxicology, 32, 31-36.

Ahmadi-Dastgerdi, A., Ezzatpanah, H., Asgary, S., Dokhani, S. & Rahimi, E. (2017). Phytochemical, Antioxidant and Antimicrobial Activity of the Essential Oil from Flowers and Leaves of Achillea millefolium subsp. millefolium. Journal of Essential Oil Bearing Plants, 20, 2, 395-409.

 

Ahmadi-Dastgerdi, A., Ezzatpanah, H., Asgary, S., Dokhani, S. & Rahimi, E. (2019). Oxidative Stability Of Mayonnaise Supplemented With Essential Oil of Achillea Millefolium Ssp Millefolium During Storage. Food Science and Technology, 13, 1, 34-41

Almajano, M. P., Delgado, M. E. & Gordon, M. H. (2007). Albumin causes a synergistic increase in the antioxidant activity of green tea catechins in oil-in water emulsions. Food Chemistry, 102, 1375-1382.

AOCS. (1998). Official methods and recommended practices of the American Oil Chemists’ Society. Method Cd 8-53. Champaign, IL: AOCS.

AOCS. (1998). Official methods and recommended practices of the American Oil Chemists’ Society. Method Cd 19-90. Champaign, IL: AOCS.

AOCS. (1998). Official methods and recommended practices of the American Oil Chemists’ Society. Method Cd 18-90. Champaign, IL: AOCS.

ASTM. (1986). Physical requirements. Guidelines for sensory evaluation laboratories, STP 913Philadelphia, Pa: American society for testing and materials.

ASTM. (1988). In Rc Storer (Ed). Standard and Sensory Evaluation of Materials and Products ASTM Manual Series. Philadelphia, Pa: American society for testing and Materials.

Baris, O., Gulluce, M., Sahin, F., Ozer, H., Ozkan, H., Smoken, M. & Zbek, T. (2006). Biological activities of the essentional oil and Methanol extract of Achillea biebersteinii Afan. (Asteraceae). Turkey Journal of Biology, 30, 65-73.

Behnam, B. & Aliakbarlo, J. (2014). Antioxidant effects of Zataria multiflora and Mentha longifolia essential oils on chicken meat stored at 4 °C. Journal of Food Research (AGRICULTURAL SCIENC), 23, 4, 533-544. [In Persian].

Barzegar, M., Taha Nejad, M., Sahari, M. A. & Naghdi Badi, H. A. (2012). Evaluation of Antioxidant Activity of Lavandula angustifolia Essential Oil in Crude Soybean Oil System. Journal of Medicinal Plants, 11, 41. [In Persian].

Beltran, G., Aguilera, M. P. & Gordon, M. H. (2005). Solid phase microextraction of volatile oxidation compounds in oil-in-water emulsions. Food Chemistry, 92, 3, 401-406.

Berton‐Carabin, C. C., Ropers, M. H. & Genot, C. (2014). Lipid Oxidation in Oil‐in‐Water Emulsions: Involvement of the Interfacial Layer. Comprehensive Reviews in Food Science and Food Safety, 13, 5, 945-977.

Bligh, E. G. & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology, 37, 8, 911-917.

Brannan, R. G. & Mah, E. (2007). Grape seed extract inhibits lipid oxidation in muscle from different species during refrigerated and frozen storage and oxidation catalyzed by peroxynitrite and iron/ascorbate in a pyrogallol red model system. Meat Science, 77, 4, 540-545.

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods-a review. Journal of Food Microbiology, 94, 223-253.

Chanda, S. & Dave, R. (2009). In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research. 3, 13, 981-996.

Fasseas, M. K., Mountzouris, K. C., Tarantilis, P. A., Polissiou, M. & Zervas, G. (2007). Antioxidant activity in meat treated with oregano and sage essential oils. Food Chemistry, 106, 1188-1194.

Fatemi, H. (1998). Food Chemistry, Enteshar sahami Compani. [In Persian].

Fazel, M., Omidbeygi, M., Barzegar, M. & Naghdi Badi, H. (2007). Influence of Heating on Antiradical Activity of Essential Oils of Thyme, Summer Savory and Clove by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH") Method. Journal of Medicinal Plants, 6, 22, 54.60. [In Persian].

Frankel, E. N., Satue-Gracia, T., Meyer, A. S. & German, J. B. (2002). Oxidative stability of fish and algae oils containing long-chain polyunsaturated fatty acids in bulk and in oil in water emulsions. Journal of Agriculture and Food Chemistry, 50, 2094-9.

Frankel, E. N. )2012(. Lipid oxidation,2^nd ed. Cambridge, UK, Woodhead Publishing Limited.

Gavahian, M., Hashemi, S. M. B., Mousavi Khaneghah, A. & Mazaheri Tehrani, M. (2013). Ohmically extracted Zenyan essential oils as natural antioxidant in mayonnaise. International Food Research Journal, 20, 6, 3189-3195.

Heinonen, M. (2007). Antioxidant activity and antimicrobial effect of berry phenolics-a finnish perspective. Molecular Nutrition & Food Research, 51, 684–691.

Hernandez-Hernandez, E., Ponce-Alquicira, E., Jaramillo-Flores, M. E. & Legarreta, G. (2009). Antioxidant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Science, 81, 410-417.

Hrasi, A., Hadolin, M., Knez, Z. & Bauman, D. (2000). Comparison of antioxidative and synergistic effects of rosemary extract with a-tocopherol, ascorbyl palmitate and citric acid in sunflower oil. Food Chemistry, 71, 229-233.

ISIRI. (2005). Permitted oral antioxidants. National Standard of Iran, No. 3608, first edition. [In Persian].

Jacobsen, C., Hartvigsen, K., Lund, P., Meyer, A., AdlerNissen, J., Holstborg, J. & Hølmer, G. (1999). Oxidation in fish-oil-enriched mayonnaise. 1. Assessment of propyl gallate as an antioxidant by discriminant partial least squares regression analysis. European Food Research Technology, 210, 13-30.

Jacobsen, C., Meyer, A. S. & Adler-Nissen, J. (1999). Oxidation mechanisms in real food emulsions: oil-water partition coefficients of selected volatile off-flavor compounds in mayonnaise. Z Lebensm Unters, 208, 317-327.

Jacobsen, C., Hartvigsen, K., Lund, P., Thomsen, M. K., Skibsted, L., Gunhild, H., AdlerNissen, J. & Meyer, A. (2001). Oxidation in fish oil-enriched mayonnaise: 4. Effect of tocopherol concentration on oxidative deterioration. European Food Reserch and Technology, 212, 308-318.

Karami, K., Heidari Jamshidi, A. & Ariapoor, A. (2013). Investigation of antibacterial properties and chemical composition of essential oil of Zataria multifora boiss (Thyme) in Crete-Lorestan province. Journal of Plant Ecophysiology, 13, 5, 64-80. [In Persian].

Kishk, Y. F. M. & Elsheshetawy, H. (2013). Effect of ginger powder on the mayonnaise oxidative stability, rheological measurements and sensory characteristics. Annals of Agricultural Science. 58, 2, 213-220.

Kulisic, T., Radonic, A. & Katalinic, V. (2004). Use of different methods for testing antioxidative of oregano essntial oil. Journal of Food Chemistry, 85, 633-40.

Kulisic, A., Katalinic, V. & Milos, M. (2004). Use of different Methods for testing antioxidative activity of oregano essential oil. Food Chemistry, 85, 633-640.

Li, C. Y., Kim, H. W., Lee, D. C. & Rhee, H. I. (2014). Antioxidative effect of purple corn extracts during storage of mayonnaise. Food chemistry, 152, 592-596.

Mattia, C. D. D., Sacchetti, G., Mastrocola, D. & Pittia, P. (2009). Effect of phenolic antioxidants on the dispersion state and chemical stability of olive oil O/W emulsions. Food Research International, 42, 1163-1170

Maqsood, S. & Benjakul, S. (2010). Comparative studies of four different phenolic compounds on in vitro antioxidative activity and the preventive effect on lipid oxidation of fish oil emulsion and fish mince. Food Chemistry, 119, 123-132.

McClements, D. J. & Decker, E. A. (2000). Lipid oxidation in oil-in water emulsions: impact of molecular environment on chemical reactions in heterogeneous food systems. Journal of Food Science, 65, 1270-128.

Mehran, M., Hoseini, H., Hatami, A., Taghizade, M. & Safaie, A. (2016). Investigation of Components of Seven Species of Thyme Essential Oils and Comparison of their Antioxidant Properties. Journal of Medicinal Plants, 15, 58, 134-140. [In Persian].

Mihov, R., Nikovska, K. R., Nenov, N. & Slavchev, A. (2012). Evaluation of mayonnaise-like food emulsions with extracts of herbs and spices. Emir. Journal of Food Agriculture, 24, 3, 191-199.

Mimica-Dukić, N., Bozin, B., Soković, M., Mihajlović, B. & Matavulj, M. (2003). Antimicrobial and antioxidant activities of three Mentha species essential oils. Planta Medica, 69, 413-419.

Najafian, S. H., Shahcheraghi, M. A. & Roshan, V. (2016). Investigation of the Effect of Extraction Duration on the Essential Oil Content and Composition of Zataria multiflora and Thymus daenensis under Flowering Stages. IJHST, 17, 2,181-192. [In Persian].

Nasiri, E., Moosavi-Nasab, M., Shekarforoush, S. S. & Golmakani, M. T. (2014). The effects of Zataria multiflora on inhibition of polyphenoloxidase and melanosis formation in shrimp (Litopenaeus vannamei. Iranian fisheris Science Journal, 23, 3,109-118. [In Persian].

Roshani, S., Gohari Ardebili, A. & Arianfar, A. (2015). Investigation on antimicrobial and antioxidant effects of Thymus vulgaris on mozzarella cheese. Journal of Research and Innovation in Food Science and Technology, 4, 3, 233-246. [In Persian].

Saleh, M. (2010). Antioxidant and free radical scavenging activities of essential oils. Ethnicity & Disease. 20.

Shahin, R., Nayebzadeh, K., Alizadeh, L. & Mohammadi, A. (2014). Antioxidant effect of tocopherol and TBHQ on oil oxidation over the shelf life of mayonnaise: Iranian Journal of Nutrition Sciences & Food Technology, 8, 4, 227-236. [In Persian].

Shahidi, F. & Wanasundara, U. N. (2002). Methods for measuring oxidative rancidity in fats and oils. Food lipids: chemistry, nutrition and biotechnology, 387-403.

Shahsavari, N., Barzegar, M., Sahari, M. & Naghdi Badi, H. (2008). An Investigation on the Antioxidant Activity of Essential Oil of Zataria multiflora Boiss in Soy Bean Oil. Journal of Medical Plants, 7, 28, 56-68. [In Persian].

Özkan, A. & Erdoğan, A. (2011). A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turkey Journal of Biology, 35, 735- 742.

Tananuwong, K. & Tewaruth, W. (2010). Extraction and application of antioxidants from black glutinous rice. LWT: Food Science and Technology, 43, 476-481.

Ündeğer, Ü., Başaran, A., Degen, G. H. & Başaran, N. (2009). Antioxidant activities of major thyme ingredients and lack of (oxidative) DNA damage in V79 Chinese hamster lung fibroblast cells at low levels of carvacrol and thymol. Food Chemistry and Toxicology, 47, 2037-2043.