Evaluation of biochemical factors and antioxidants derived from clone 100 Iranian tea and study on HCT-116 cell colon cancer in BALB /c mice

Subject Areas : Nut

shahrzad rahnama ¹ , Asa Ebrahimi ² , Forozandeh Mahjobi ³ , mahmmod khosrow shahli ⁴ , Mahdi Rahaie ⁵

1 - Ph.D Student of the Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor of the Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor of the Department of Medical Genetics, National Institute of Genetics and Biotechnology; Karaj, Iran.
4 - Associate Professor of the Department of Medical Genetics, National Institute of Genetics and Biotechnology, Karaj, Iran.
5 - Associate Professor of the Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Iran.

Received: 2021-12-28 Accepted : 2022-02-23 Published : 2022-09-23

Keywords: Catechin, tea, Free Radicals, Colorectal Cancer, Chromatography,

Abstract :

Introduction: Tea is one of the most popular drinks in the world. It contains concentrations of antioxidants and polyphenols that might be of interest to human health. The aim of this study was to investigate the properties of antioxidants and polyphenols and the effectiveness of hydro-methanol extract of WT on the growth of colon cancer tumors in BALB/c. Materials and Methods: In order to measure the amount of polyphenols and antioxidants in different types of tea, experiments were performed. Extract of different types of tea was obtained by different methods and in order to determine the free radical scavenging capacity separated antioxidants by HPLC, were evaluated by DPPH. The phenolic content of the extract was measured using Folin-siocalto. Then, BALB/c mice with the same initial weight were induced in the colon cancer model using HCT-116 cell line. All groups were treated with two concentrations of the extract. The lowest dose was 15mg/kg and the highest was 150mg/kg. Different traits were evaluated in mice and the data were analyzed by one-way test.hydro-methanol method was the most effective in terms of antioxidant content and had a significant difference with other methods, white tea extract showed the highest and strongest antioxidant properties and the highest of free radical scavenging. The results of animal studies showed that the difference in secondary weight from the primary in mice receiving the extract with a high dose compared to the mice receiving the low dose increased significantly (P<0.05). However, tumor size, area and tumor weight at high dose were significantly reduced as compared to the low dose.hydro-methanol extraction method has the highest antioxidant activity and the highest specific catechins and according to the results, extract WT can have reducing effects in the process and control of colon cancer in infected mice.

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