Proximate composition and protein electrophoresis pattern of muscle from black mouth croaker (Atrobucca nibe) in the Oman Sea

Subject Areas : Food Science and Technology

S.P Hosseini-Shekarabi ¹ , S.E Hosseini ² , مهدی Soltani ³ , ابولقاسم Kamali ⁴ , تورج Valinassab ⁵

1 - PhD student, Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor, Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Professor, Department of aquatic animal health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
4 - Professor, Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Professor, Iranian Fisheries Researches Organization, Tehran, Iran.

Received: 2013-07-22 Accepted : 2013-10-15 Published : 2013-05-22

Keywords: chemical composition, electrophoresis, Fatty acids profile, Black mouth croaker,

Abstract :

   Black mouth croaker is considered as a new and valuable resource in the deepwater of the Oman Sea. This study describes the chemical proximate composition, the fatty acid profiles of the fish oil using gas chromatography and phosphorus element (colorimetric method) of the fish muscle. Moreover, the pattern of protein using polyacrylamide gel electrophoresis (SDS-PAGE) was carried out. Proximate composition of the fillet was calculated in wet weight as follow: 79.32±0.299% moisture, 18.19±0.145% protein, 1.01±0.032% fat and 1.434±0.047% ash. The concentration of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were measured 39.54±0.272%, 37.66±0.161% and 23.56±0.369%, respectively. Among PUFA, concentration of DHA (9.43±0.345%) was obtained more than EPA (1.16±0.051%) (p<0.05). Phosphorus content was 260.73±0.134 mg/100 wet weight. Based on SDS-PAGE, the 11 protein bands in the range of 10 to 200 kDa were detected. Myosin heavy chain (MHC) showed the most dense band. According to high nutritional value and lean-flesh of black mouth croaker, this species may be appropriate for the production of frozen fillet and surimi.

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