Distribution of genes encoding biofilm production in S. aureus isolated from raw milk in Kurdistan

Subject Areas : Food Science and Technology

مهسا Shojaei ¹ , هیوا Karimi Darehabi ² , جوادی javadi ³

1 - M.Sc Student in food science and technology, Science and Research Branch, Islamic Azad University, Sanandaj, Iran.
2 - Assistant Professor of Food Hygiene Department, ‍College of Veterinary Science, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
3 - Associate Professor of Food Hygiene Department, ‍College of Veterinary Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2013-11-09 Accepted : 2014-08-29 Published : 2014-08-23

Keywords: Staphylococcus aureus, raw milk, biofilms, Sanandaj,

Abstract :

   Staphylococcus aureus is a major cause of bovine mastitis. Several virulence factors are involved in mastitis. One of the important virulence factors is the ability of the bacterium to produce biofilms. These bacteria are capable of producing polysaccharides and proteinaceous substances attached to the surfaces, leads to biofilm formation. In this study, a total of 120 raw milk samples was obtained from Kurdistan province and analyzed for the presence of S. aureus. The presence of S. aureus was assessed by conventional culture method and confirmed by PCR assay. For this, nuc gene was exploited as the specific target sequence to detect S. aureus.  Moreover, Multiplex PCR was used to identify the presence of clfaB, fnbA, icaD and icaA genes which encode biofilm formation. Based on results, S. aureus was found in 49 (40.83%) of the samples. The frequency of each of the genes was determined as 69.38%, 32.6%, 38.77%, and 59.18 for fnbA, clfaB, icaD and icaA, respectively. Results revealed a high contamination rate of raw milks with S. aureus, and the ability of the isolates to form biofilms.

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