Survey on the contamination rate of Sarcocystis in slaughtered sheep by digestive method
Subject Areas : Food Science and Technology
1 - Graduated in Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
2 - Associate Professor, Department of Parasitology, Urmia Branch, Islamic Azad University, Urmia, Iran
Keywords: Sheep, digestive method, sarcocystosis, khowy,
Abstract :
Sarcocystis is an obligatory intracellular protozoan parasite that can cause digestive disorders in infected patients. This study aimed to determine the incidence of Sarcocystis infection in slaughtered sheep of the Khoy abattoir, Iran. For this, 204 sheep carcasses were examined by visual observation and then further analyzed by digestion method. Based on the results, 5.88% of the studied carcasses were diagnosed with macrocytic observation. In the microscopic examination, 42.65% of the cases were found positive. Data analysis showed a statistically significant difference between contamination rate in different age groups. The occurrence rate increased with age (P < 0.05), while the intensity of infection was independent of the gender. There was also a statistically significant difference between the contamination rate among different muscles (p <0.05). In other words, the highest microcyst contamination observed in skeletal muscles (90.80%). Meanwhile, the lowest microcyst contamination was observed in the heart muscle (29.88%). This study showed the necessity of microscopic observation of the Sarcocystis contamination in the sheep carcasses.
References:
· Berenji, F., Behniafar, H., Zabolinejad, N., Fata, A.M., Salehi, M., Sadabadi, F. (2019). Prevalence of Sarcocystis infection in slaughtered sheep by macroscopic and histopathologic method in Mashhad. Medical Journal of Mashhad University of Medical Sciences, 62(3): 1556-1561.
· Parandin, F., Feizi, F., Maghsood, A.H., Matini, M., Roshan, A., Fallah, M. (2015). A Survey on Sarcocystis Infection Rate in Slaughtered Cattle and Sheep by Macroscopic Inspection and Pepsin Digestion Methods in Hamadan Abattoir. Scientific Journal of Hamadan University of Medical Sciences, 22(3): 210-216.
· Alibeigi, Z., Rahbari, S., HoghooghiRad, N., Naisi, S. (2015). Macroscopic and microscopic survey of sarcocystosis in ruminants Shahriar slaughterhouse, during 2012-2013. Journal of Veterinary Research, 70(4): 441-445.
· Anja, H., Tenter, R., Astrie, M. and Tokai, J. (1999). Comparision of Immunologicil and Molecular Methods for the Diagnosis of Infections with Pathogenic Sarcocystis species in Sheep. Clinical Medicine, 23(6): 293-30.
· Beyazit, A., Yazicioglu O., Karear, Z. (2007). The prevalence of ovine Sarcocystis species in Izmir province. Ankara University Veterinary Faculty Department, 54, 111-116, 2007.
· Bonyadin, M. And Mashki, b. (2006). Evaluation of contamination of carcasses of cows slaughtered in Shahrekord slaughterhouse to sarcophagus. Research and construction, pp. 14-18. [In Persian [
· Dalimi, A., Arshad, M. and GhaffariFar, F. (2008). Assessment of Sarcocystis infection in slaughtered goats by different methods. Pajouhesh and Sazandegi, 21(4): 38-42. [In Persian[
· Dalimi, A., Arshad, M., Ghaffarifar, F. (2009). Assessment of Sarcocystis infection in slaughtered.. goats by different methods. Animal and Fisheries Sciences, 21(4): 38-42.
· Dubey, J., Saville, W.A., Lindsay, D., Stich. R., Stanek, J., Speer, C. et al. (2000). Completion of the life cycle of Sarcocystis neurona. Journal of Parasitology, 86(6): 1276-80.
· Dubey, J.P., Speer, CA. and Fayer, R. (1989). Sarcocystis in animals and Man. 1st ed. Florida: CRC Press, Boca Raton, p. 215.
· Emnett, C.W. and Hugghins, E.J. (1982). Sarcocystis of deer in South Dakota. Journal of Wildlife Disease, 18(2): 187-93.
· Fayer, R. (2004). Sarcocystis spp in human infections. Clinical Microbiology Reviews 17(4): 894- 902.
· Fayer, R., Esposito, D.H. and Dubey, J. (2015). Human infections with Sarcocystis species. Clinical Microbiology Reviews, 28(2): 295-311.
· Gabriele, G., Robba,S., Germani,O. and Scanziani,E. (2006). Identification and prevalence of Sarcocystis spp. Cysts in bovine canned meat. Food Control, 17( 9): 691-694
· Hamidinejat, H., Razi Jalali, M., Nabavi, L. (2010). Survey on Sarcocystis infection in slaughtered cattle in south-west of Iran, emphasized on evaluation of muscle squash in comparison with digestion method. Journal of Animal and Veterinary Advances, 9(12): 1724-1726.
· Hemmati, R., Shahbari, A., Fallah, A., Mohammadi, M. and Esfaram, V. (2013). The prevalence of sarcocystis contamination in slaughtered cows in Tabriz. Comparative Pathology, 4: 1095-1100. [In Persian[
· Kargar Jahromi, Z., Solhjoo, K., Zareian Jahromi, M., Kargar Jahromi, H., Erfanian, S. And nominal, m. (2012), Investigation of Sarcosist Infection in Goats slaughtered in Jahrom slaughterhouse. Journal of Fasa University of Medical Sciences, 2(3): 163-167. [In Persian[
· Kirkpatrick, C., Dubey, J.P.,Goldschmidt, M.H. and Saik, J.E. (1986). Sarcocystis sp in muscles of domestic cats. Veterinary Pathology, 23(1): 88-90.
· Latif, B., Al-Delemi, J., Mohammed, B., Al-Bayati, S.and Al-Amiry, A.(1999) Prevalence of Sarcocystis spp. in meat- producing animals in Iraq. Veterinary Pathology, 84(1-2): 85-90
Mirzaei Dehaghi, M., Fallahi, M., Sami, M., Radfar, M.H. (2012). Survey of Sarcocystis in fection in slaughtered sheep Abattoir Kerman. Comparative Clinical Pathology, 21: 1991-2012.
· Mirzaei Dehaghi, M., Fallahi, M., Sami, M., Radfar, M.H. (2012) Survey of sarcocysyis infection in slaughtered sheep in Kerman abattoir, Kerman, Iran. Comparative Clinical Pathology. 22: 343-346.
Mirzaei, M., Rezaei, H. (2014). The role of sheep in the epidemiology of Sarcocystis spp. in Tabriz area northwest of Iran. Journal of Parasitic Diseases, 40(2): 285-288.
· Mokhtarian, K., Khalili, B., Karimi, I., Yazdanparast, M., Kathiri, K., Tarshizi, R. et al. (2010). Investigation of Sarcocystitis Contamination in Animals Killed in the Slaughterhouse of Kurd City inSummer 2007 by Histopathological Method. Journal of Shahrekord University of Medical Sciences, 23(1): 32-36. [In Persian [
· Nourollahi Fard, SR., Asghari, M. and Nouri, F. (2009). Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Tropical Animal Health and Production; 41(8):1633-1636.
· Razavi, S.M., Shekarforoush, S.S., Farahani, M. and Sarihi, K. (2003). Prevalence of sarcocyst in slaughtered sheep in Shiraz, Iran. Journal of Veterinary Parasitology, 17(2): 139-141.
· Shekarforoush, S.S., Razavi, S.M. and Dehghan, S.A. (2005). Prevalence of Sarcocystis species in slaughtered goats in Shiraz, Iran. Veterinary Record, 156(13): 418-420.
· Svobodova, V. and Nevole, M. (1990). Use of muscle digestion method and indirect immunofluorescence reaction in the diagnosis of sarcocystosis in sheep. Acta Veterinaria Brno, 59(13): 157-170.
· Tenter, A.M. (1995). Current research on Sarcocystis species of domestic animals. International Journal for Parasitology. 25(11): 131-130.
· Wouda, W., Snoep,J. and Dubey, J.P.(2006). Eosinophilic myositis due to sarcocystis hominis in a beef cow. Journal of Comparative Pathology, 135(4): 249-253.
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· Berenji, F., Behniafar, H., Zabolinejad, N., Fata, A.M., Salehi, M., Sadabadi, F. (2019). Prevalence of Sarcocystis infection in slaughtered sheep by macroscopic and histopathologic method in Mashhad. Medical Journal of Mashhad University of Medical Sciences, 62(3): 1556-1561.
· Parandin, F., Feizi, F., Maghsood, A.H., Matini, M., Roshan, A., Fallah, M. (2015). A Survey on Sarcocystis Infection Rate in Slaughtered Cattle and Sheep by Macroscopic Inspection and Pepsin Digestion Methods in Hamadan Abattoir. Scientific Journal of Hamadan University of Medical Sciences, 22(3): 210-216.
· Alibeigi, Z., Rahbari, S., HoghooghiRad, N., Naisi, S. (2015). Macroscopic and microscopic survey of sarcocystosis in ruminants Shahriar slaughterhouse, during 2012-2013. Journal of Veterinary Research, 70(4): 441-445.
· Anja, H., Tenter, R., Astrie, M. and Tokai, J. (1999). Comparision of Immunologicil and Molecular Methods for the Diagnosis of Infections with Pathogenic Sarcocystis species in Sheep. Clinical Medicine, 23(6): 293-30.
· Beyazit, A., Yazicioglu O., Karear, Z. (2007). The prevalence of ovine Sarcocystis species in Izmir province. Ankara University Veterinary Faculty Department, 54, 111-116, 2007.
· Bonyadin, M. And Mashki, b. (2006). Evaluation of contamination of carcasses of cows slaughtered in Shahrekord slaughterhouse to sarcophagus. Research and construction, pp. 14-18. [In Persian [
· Dalimi, A., Arshad, M. and GhaffariFar, F. (2008). Assessment of Sarcocystis infection in slaughtered goats by different methods. Pajouhesh and Sazandegi, 21(4): 38-42. [In Persian[
· Dalimi, A., Arshad, M., Ghaffarifar, F. (2009). Assessment of Sarcocystis infection in slaughtered.. goats by different methods. Animal and Fisheries Sciences, 21(4): 38-42.
· Dubey, J., Saville, W.A., Lindsay, D., Stich. R., Stanek, J., Speer, C. et al. (2000). Completion of the life cycle of Sarcocystis neurona. Journal of Parasitology, 86(6): 1276-80.
· Dubey, J.P., Speer, CA. and Fayer, R. (1989). Sarcocystis in animals and Man. 1st ed. Florida: CRC Press, Boca Raton, p. 215.
· Emnett, C.W. and Hugghins, E.J. (1982). Sarcocystis of deer in South Dakota. Journal of Wildlife Disease, 18(2): 187-93.
· Fayer, R. (2004). Sarcocystis spp in human infections. Clinical Microbiology Reviews 17(4): 894- 902.
· Fayer, R., Esposito, D.H. and Dubey, J. (2015). Human infections with Sarcocystis species. Clinical Microbiology Reviews, 28(2): 295-311.
· Gabriele, G., Robba,S., Germani,O. and Scanziani,E. (2006). Identification and prevalence of Sarcocystis spp. Cysts in bovine canned meat. Food Control, 17( 9): 691-694
· Hamidinejat, H., Razi Jalali, M., Nabavi, L. (2010). Survey on Sarcocystis infection in slaughtered cattle in south-west of Iran, emphasized on evaluation of muscle squash in comparison with digestion method. Journal of Animal and Veterinary Advances, 9(12): 1724-1726.
· Hemmati, R., Shahbari, A., Fallah, A., Mohammadi, M. and Esfaram, V. (2013). The prevalence of sarcocystis contamination in slaughtered cows in Tabriz. Comparative Pathology, 4: 1095-1100. [In Persian[
· Kargar Jahromi, Z., Solhjoo, K., Zareian Jahromi, M., Kargar Jahromi, H., Erfanian, S. And nominal, m. (2012), Investigation of Sarcosist Infection in Goats slaughtered in Jahrom slaughterhouse. Journal of Fasa University of Medical Sciences, 2(3): 163-167. [In Persian[
· Kirkpatrick, C., Dubey, J.P.,Goldschmidt, M.H. and Saik, J.E. (1986). Sarcocystis sp in muscles of domestic cats. Veterinary Pathology, 23(1): 88-90.
· Latif, B., Al-Delemi, J., Mohammed, B., Al-Bayati, S.and Al-Amiry, A.(1999) Prevalence of Sarcocystis spp. in meat- producing animals in Iraq. Veterinary Pathology, 84(1-2): 85-90
Mirzaei Dehaghi, M., Fallahi, M., Sami, M., Radfar, M.H. (2012). Survey of Sarcocystis in fection in slaughtered sheep Abattoir Kerman. Comparative Clinical Pathology, 21: 1991-2012.
· Mirzaei Dehaghi, M., Fallahi, M., Sami, M., Radfar, M.H. (2012) Survey of sarcocysyis infection in slaughtered sheep in Kerman abattoir, Kerman, Iran. Comparative Clinical Pathology. 22: 343-346.
Mirzaei, M., Rezaei, H. (2014). The role of sheep in the epidemiology of Sarcocystis spp. in Tabriz area northwest of Iran. Journal of Parasitic Diseases, 40(2): 285-288.
· Mokhtarian, K., Khalili, B., Karimi, I., Yazdanparast, M., Kathiri, K., Tarshizi, R. et al. (2010). Investigation of Sarcocystitis Contamination in Animals Killed in the Slaughterhouse of Kurd City inSummer 2007 by Histopathological Method. Journal of Shahrekord University of Medical Sciences, 23(1): 32-36. [In Persian [
· Nourollahi Fard, SR., Asghari, M. and Nouri, F. (2009). Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Tropical Animal Health and Production; 41(8):1633-1636.
· Razavi, S.M., Shekarforoush, S.S., Farahani, M. and Sarihi, K. (2003). Prevalence of sarcocyst in slaughtered sheep in Shiraz, Iran. Journal of Veterinary Parasitology, 17(2): 139-141.
· Shekarforoush, S.S., Razavi, S.M. and Dehghan, S.A. (2005). Prevalence of Sarcocystis species in slaughtered goats in Shiraz, Iran. Veterinary Record, 156(13): 418-420.
· Svobodova, V. and Nevole, M. (1990). Use of muscle digestion method and indirect immunofluorescence reaction in the diagnosis of sarcocystosis in sheep. Acta Veterinaria Brno, 59(13): 157-170.
· Tenter, A.M. (1995). Current research on Sarcocystis species of domestic animals. International Journal for Parasitology. 25(11): 131-130.
· Wouda, W., Snoep,J. and Dubey, J.P.(2006). Eosinophilic myositis due to sarcocystis hominis in a beef cow. Journal of Comparative Pathology, 135(4): 249-253.
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