Glycation of nisin with glucose, Lactose and dextran and investigation of its inhibitory effect on Escherichia coli and Salmonella Typhimurium
Subject Areas :
Food Science and Technology
M. Hashempour Sadeghian
1
,
N. Kazemipour
2
,
S. Shekarforoush
3
,
M. H. Eskandari
4
1 - Student of biochemistry, Department of basic science, School of veterinary medicine, Shiraz University, Shiraz, Iran
2 - Associate professor of biochemistry, Department of basic science, School of veterinary medicine, Shiraz University, Shiraz, Iran
3 - Professor of Food hygiene, Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
4 - Professor of Food hygiene, Department of Food science and technology, School of agriculture, Shiraz University, Shiraz, Iran
Received: 2017-09-02
Accepted : 2017-12-28
Published : 2018-12-22
Keywords:
Nisin,
gram-negative,
Glycation,
Maillard reaction,
Abstract :
Nisin is an antimicrobial peptide used in the food industry as a preservative. However, this peptide has no considerable effect on gram-negative bacteria. In this study, the effect of glycation on the antimicrobial activity of nisin was elucidated against Escherichia coli and Salmonella Typhimurium. A solution of nisin and fivefold concentration of glucose, lactose and dextran and solution of nisin without any sugar were prepared in phosphate buffer and were lyophilized. The lyophilized powder was exposed to 60°C temperature and 70% humidity for 7 days. Every 24 hours, one sample was collected and dissolved in distilled. The equal molar concentration of native and conjugated nisin was made. Percentage of glycation was measured by OPA (ortho-phthalaldehyde) method. MIC50 of nisin was assayed by microdilution method against E. coli and S. Ttyphimurium. The result of this study has revealed that the percentage of glycation is conversely related to the size of carbohydrates in which nisin-glucose had the highest and nisin-dextran had the least percentage of glycation. Glycation of nisin increased the MIC50 of nisin against E. coli after seven days. MIC50 of native nisin and glycated nisin had no difference against S. Typhimurium. From this study, it was concluded that conjugation of nisin with carbohydrates is not able to extend the antimicrobial activity of nisin to gram-negative bacteria.
References:
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· Muppalla, R., Sonavale, R., Chawla, S. and Sharma, A. (2012). Functional properties of nisin–carbohydratec onjugates formed by radiation induced Maillard reaction. Radiation Physics and Chemistry, 81(12): 1917-1922.
· Nielsen, P., Petersen, D. and Dambmann, C. (2001). Improved method for determining food protein degree of hydrolysis. Journal of food science, 66(5): 642-646.
· Prudêncio, C.V., Dos Santos, M.T. and Vanetti, M.C.D. (2015). Strategies for the use of bacteriocins in Gram-negative bacteria: relevance in food microbiology. Journal of food science and technology, 52(9): 5408-5417.
· Sahl, H.G., Kordel, M. and Benz, R. (1987). Voltage-dependent depolarization of bacterial membranes and artificial lipid bilayers by the peptide antibiotic nisin. Arch Microbiol, 149(2): 120-124.
· Sant’Anna, V., Cladera-Olivera, F. and Brandelli, A. (2012). Kinetic and thermodynamic study of thermal inactivation of the antimicrobial peptide P34 in milk. Food Chemistry, 130(1): 84-89.
· Sato, R., Sawabe, T. and Saeki, H. (2005). Characterization of fish myofibrillar protein by conjugation with alginate oligosaccharide prepared using genetic recombinant alginate lyase. Journal of food science, 70(1): C58-C62.
· Stevens, K., Sheldon, B., Klapes, N. and Klaenhammer, T. (1991). Nisin treatment for inactivation of Salmonella species and other gram-negative bacteria. Applied and Environmental Microbiology, 57(12): 3613-3615.
· Usui, M., Tamura, H., Nakamura, K., Ogawa, T., Muroshita, M., Azakami, H., et al. (2004). Enhanced bactericidal action and masking of allergen structure of soy protein by attachment of chitosan through Maillard‐type protein‐polysaccharide conjugation. Food/Nahrung, 48(1): 69-72.
· Viedma, P.M., López, A.S., Omar, N.B., Abriouel, H., López, R.L., Valdivia, E., et al. (2008). Enhanced bactericidal effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against Salmonella enterica in apple juice. International Journal of food microbiology, 128(2): 244-249.
· Zhou, L., van Heel, A.J., Montalban-Lopez, M. and Kuipers, O.P. (2016). Potentiating the activity of nisin against Escherichia coli. Frontiers in cell and developmental biology, 4(7): 1-9.
· Zhu, D., Damodaran, S. and Lucey, J.A. (2010). Physicochemical and emulsifying properties of whey protein isolate (WPI)− dextran conjugates produced in aqueous solution. Journal of agricultural and food chemistry, 58(5): 2988-2994.
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· Abdullah, S.U., Badaruddin, M., Ali, R. and Riaz, M.N. (2010). Effect of elementary and advanced glycation products of nisin on its preservative efficacy and digestibility. Food Chemistry, 122(4): 1043-1046.
· Boziaris, I. and Adams, M. (1999). Effect of chelators and nisin produced in situ on inhibition and inactivation of Gram negatives. International Journal of food microbiology, 53(2): 105-113.
· Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(248-254.
· Breukink, E., van Heusden, H.E., Vollmerhaus, P.J., Swiezewska, E., Brunner, L., Walker, S., et al. (2003). Lipid II is an intrinsic component of the pore induced by nisin in bacterial membranes. Journal of Biological Chemistry, 278(22): 19898-19903.
· Breukink, E., van Kraaij, C., Demel, R.A., Siezen, R.J., Kuipers, O.P. and de Kruijff, B. (1997). The C-terminal region of nisin is responsible for the initial interaction of nisin with the target membrane. Biochemistry, 36(23): 6968-6976.
· Cao-Hoang, L., Marechal, P., Le-Thanh, M. and Gervais, P. (2008). Synergistic action of rapid chilling and nisin on the inactivation of Escherichia coli. Applied Microbiology and Biotechnology, 79(1): 105-109.
· Casey, J., O'Cleirigh, C., Walsh, P. and O'Shea, D. (2004). Development of a robust microtiter plate-based assay method for assessment of bioactivity. Journal of microbiological methods, 58(3): 327-334.
· Chen, H., Davidson, P.M. and Zhong, Q. (2014). Antimicrobial properties of nisin after glycation with lactose, maltodextrin and dextran and the thyme oil emulsions prepared thereof. International Journal of food microbiology, 191: 75-81.
· Cleveland, J., Montville, T.J., Nes, I.F. and Chikindas, M.L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of food microbiology, 71(1): 1-20.
· Driessen, A.J., van den Hooven, H.W., Kuiper, W., van de Kamp, M., Sahl, H.G., Konings, R.N., et al. (1995). Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles. Biochemistry, 34(5): 1606-1614.
· Elliason, D. and Tatini, S. (1999). Enhanced inactivation of Salmonella typhimurium and verotoxigenic Escherichia coli by nisin at 6· 5° C. Food Microbiology, 16(3): 257-267.
· Gerrard, J., Brown, P. and Fayle, S. (2003). Maillard crosslinking of food proteins III: the effects of glutaraldehyde, formaldehyde and glyceraldehyde upon bread and croissants. Food Chemistry, 80(1): 45-50.
· Govaris, A., Solomakos, N., Pexara, A. and Chatzopoulou, P. (2010). The antimicrobial effect of oregano essential oil, nisin and their combination against Salmonella Enteritidis in minced sheep meat during refrigerated storage. International Journal of food microbiology, 137(2): 175-180.
· Khan, I. and Oh, D.-H. (2016). Integration of nisin into nanoparticles for application in foods. Innovative Food Science & Emerging Technologies, 34(376-384.
· Lertittikul, W., Benjakul, S. and Tanaka, M. (2007). Characteristics and antioxidative activity of Maillard reaction products from a porcine plasma protein–glucose model system as influenced by pH. Food Chemistry, 100(2): 669-677.
· Liu, J., Ru, Q. and Ding, Y. (2012). Glycation a promising method for food protein modification: physicochemical properties and structure, a review. Food Research International, 49(1): 170-183.
· Masschalck, B., Garcı́, C., Van Haver, E. and Michiels, C.W. (2000). Inactivation of high pressure resistant Escherichia coli by lysozyme and nisin under high pressure. Innovative Food Science & Emerging Technologies, 1(1): 39-47.
· Medrano, A., Abirached, C., Panizzolo, L., Moyna, P. and Añón, M. (2009). The effect of glycation on foam and structural properties of β-lactoglobulin. Food chemistry, 113(1): 127-133.
· Molinos, A.C., Abriouel, H., López, R.L., Valdivia, E., Omar, N.B. and Gálvez, A. (2008). Combined physico-chemical treatments based on enterocin AS-48 for inactivation of Gram-negative bacteria in soybean sprouts. Food and chemical toxicology, 46(8): 2912-2921.
· Moosavy, M.-H., Basti, A.A., Misaghi, A., Salehi, T.Z., Abbasifar, R., Mousavi, H.A.E., et al. (2008). Effect of Zataria multiflora Boiss. essential oil and nisin on Salmonella typhimurium and Staphylococcus aureus in a food model system and on the bacterial cell membranes. Food Research International, 41(10): 1050-1057.
· Mulders, J.W., Boerrigter, I.J., ROLLEMA, H.S., SIEZEN, R.J. and VOS, W.M. (1991). Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. European Journal of Biochemistry, 201(3): 581-584.
· Muppalla, R., Sonavale, R., Chawla, S. and Sharma, A. (2012). Functional properties of nisin–carbohydratec onjugates formed by radiation induced Maillard reaction. Radiation Physics and Chemistry, 81(12): 1917-1922.
· Nielsen, P., Petersen, D. and Dambmann, C. (2001). Improved method for determining food protein degree of hydrolysis. Journal of food science, 66(5): 642-646.
· Prudêncio, C.V., Dos Santos, M.T. and Vanetti, M.C.D. (2015). Strategies for the use of bacteriocins in Gram-negative bacteria: relevance in food microbiology. Journal of food science and technology, 52(9): 5408-5417.
· Sahl, H.G., Kordel, M. and Benz, R. (1987). Voltage-dependent depolarization of bacterial membranes and artificial lipid bilayers by the peptide antibiotic nisin. Arch Microbiol, 149(2): 120-124.
· Sant’Anna, V., Cladera-Olivera, F. and Brandelli, A. (2012). Kinetic and thermodynamic study of thermal inactivation of the antimicrobial peptide P34 in milk. Food Chemistry, 130(1): 84-89.
· Sato, R., Sawabe, T. and Saeki, H. (2005). Characterization of fish myofibrillar protein by conjugation with alginate oligosaccharide prepared using genetic recombinant alginate lyase. Journal of food science, 70(1): C58-C62.
· Stevens, K., Sheldon, B., Klapes, N. and Klaenhammer, T. (1991). Nisin treatment for inactivation of Salmonella species and other gram-negative bacteria. Applied and Environmental Microbiology, 57(12): 3613-3615.
· Usui, M., Tamura, H., Nakamura, K., Ogawa, T., Muroshita, M., Azakami, H., et al. (2004). Enhanced bactericidal action and masking of allergen structure of soy protein by attachment of chitosan through Maillard‐type protein‐polysaccharide conjugation. Food/Nahrung, 48(1): 69-72.
· Viedma, P.M., López, A.S., Omar, N.B., Abriouel, H., López, R.L., Valdivia, E., et al. (2008). Enhanced bactericidal effect of enterocin AS-48 in combination with high-intensity pulsed-electric field treatment against Salmonella enterica in apple juice. International Journal of food microbiology, 128(2): 244-249.
· Zhou, L., van Heel, A.J., Montalban-Lopez, M. and Kuipers, O.P. (2016). Potentiating the activity of nisin against Escherichia coli. Frontiers in cell and developmental biology, 4(7): 1-9.
· Zhu, D., Damodaran, S. and Lucey, J.A. (2010). Physicochemical and emulsifying properties of whey protein isolate (WPI)− dextran conjugates produced in aqueous solution. Journal of agricultural and food chemistry, 58(5): 2988-2994.
