Effect of wortmanin on the expression of BDNF and NT3 neuroprotective cytokines in cultured Astrocyt
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganismsamir yazdanpanah 1 , vahid hemayatkhahjahromi 2
1 - Islamic Azad University, Jahrom brunch, Department of Biology, Jahrom, Iran.
2 - Islamic Azad University, Jahrom brunch, Department of Biology, Jahrom, Iran.
Keywords: BDNF, NT3, Astrocyte, Vertmanine, Neurodegenerative Diseases,
Abstract :
Background and Aim: Neurodegenerative diseases induce apoptosis in neurons by affecting the central nervous system. Glia cells play an important role in preventing the progression of these diseases and increasing neuronal survival. The most important function of glia cells after neuronal survival is the secretion of neuroprotective factors. Neuroprotective factors secreted by astrocytes include TGFβ1, TGFβ2, GDNF, BDNF, and NT3.In this study, we investigate the possible role of vortmanine in the expression of neuroprotective factors BDNF and NT3 secreted by microglia and oligodendrocytes in vitro. For this purpose, cultured microglia and oligodendrocytes were treated with concentrations of 8,12,16 μmol / L for 72 hours. materials and methods :A 1-2-day-old Wistar rat was used to culture astrocyte cells. Thus, after disinfecting the skin of the body and hands of infants with 70% alcohol-soaked cotton, the infant's head was cut with scissors, the scalp was completely removed and the skull was cut from the neck to the forehead. To reveal the cerebral cortex. The brain was then removed from the skull and placed in Hanks buffer.Concentrations of 8, 12, and 16 mol / L of vortmanine are added by sampler to microglia and oligodendrocyte cells cultured in 6-well plates and The expression of neuroprotective factors was measured by R-T PCRResults :Vertmanine-treated astrocyte cells showed significant morphological changes.However, morphological changes were much less observed in control cells compared to treated cells.
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