Protective effect of geraniol against methylation-induced lesions in testicular tissue by focusing on sperm morphology and its parameters
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Raheleh Rezaei
1
,
عباسعلی دهپورجویباری
2
*
,
محمد حسینی
3
,
مریم غلامی تبار طبری-جدید
4
*
,
فرخنده نعمتی
5
1 -
2 -
3 - Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran
4 - Health Reproductive Research Center, Sari Branch, Islamic Azad University, Sari, Iran.
5 - گروه زیست شناسی دانشکده علوم پایه دانشگاه آزاد اسلامی واحد قائمشهر، ایران
Keywords: Geraniol, Testis, Tissue methylation, Sperm, Rat,
Abstract :
Introduction: Polycyclic aromatic hydrocarbons (PAHs) have been shown to cause tissue lesions in rodents, leading to immunosuppressive effects. The mutagenic properties of these substances on reproductive system cells are also noteworthy. Geraniol (Ge), a phenolic compound, has antioxidant properties and significant potential in preventing damage caused by these toxicants.
Methods: In this 8-week experimental study, male rats were randomly assigned to four groups of five. Dimethylbenzanthracene (DMBA) was administered as a single dose of 30 mg per animal via intraperitoneal injection on the first day to induce the lesion. Ge was also administered as a weekly dose of 100 mg/kg via gavage. At the end of the period, sperm samples were collected from the epididymis and sperm parameters and morphological analysis were performed.
Results: Our findings showed that DMBA resulted in reduced sperm count and motility, increased mortality, and abnormalities in sperm morphology such as tail abnormalities, tail twist, tail shortening, microcephaly, and tail bending. Ge also reduced lesions in the treatment group.
Conclusion: Ge effectively reduced lesions in sperms by reducing oxidative stress and inflammation, highlighting its potential as a protective agent against PAH-induced damage.
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