Reducing the toxicity of zinc nanoparticles in Hypericum perforatum L. with salicylic acid and sodium nitroprusside pretreatment
Subject Areas : Tension
Elham Ghasemifar
1
(
Department of Biology Payam Noor University (PNU) PO BOX 19395-3697 Tehran, Iran,
)
Ghader Habibi
2
(
Department of Biology, Payam Noor University (PNU), PO BOX 19395-3697 Tehran, Iran
)
Keywords: antioxidants, salicylic acid, toxicity, Hypericum, zinc oxide nanoparticles, nitric oxide,
Abstract :
In the current study, the toxicity of zinc oxide nanoparticles (ZnONPs) and zinc sulfate (ZnSO4) in Hypericum perforatum L. pre-treated with salicylic acid and nitric oxide (sodium nitroprusside) at a concentration of 0.1 mM was investigated in a completely randomized design with three replicates as pot culture in perlite. After 21 days of treatment, the plants were harvested. The results of the variance analysis of the data in the laboratory showed that the application of 1000 mgL-1 of zinc oxide or zinc sulfate nanoparticles caused more toxicity. However, the greatest effect is related to the pretreatment of salicylic acid and nitric oxide (sodium nitroprusside), which successfully reduced the value of the oxidative stress index (malondialdehyde) and as a result, the adverse effect of zinc on the seed germination of gerbera. The combination of salicylic acid and nitric oxide increased the amount of proline in the treatment of zinc oxide nanoparticles and phenol antioxidants had a significant increase under the effect of zinc oxide nanoparticles and zinc sulfate, which was associated with an increase in the activity of the phenylalanine ammoni alyase enzyme. The amount of zinc in the roots also increased with the combination of salicylic acid and nitric oxide under the stress of zinc oxide nanoparticles. In this research, it was tried to minimize the negative effect of zinc oxide nanoparticles and zinc sulfate on plant products by designing new methods.