Determination Of Secondary Materials In Leaves And Orange Fruit (Thompson) In Four Geographical Directions Crown Of The Tree In Golestan Province

Subject Areas : Genetic

Saeed Danesh ¹ (Department of Medicinal Plants, General Department of Education, Golestan Province, Gorgan, Iran.)
Khodayar Hemmati ² (Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran)
Sadegh Atashi ³ (Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran)

Received: 2020-09-01 Accepted : 2020-10-22 Published : 2021-12-22

Keywords: HPLC, Spectrophotometer, Orange, geographical direction, secondary substances,

Abstract :

Orange (Citrus sinensis L.) is a tree of which leaves, flowers, fruit and fruit skin lesions have been used in different industries including cosmetics, health and food industries. Orange fruit rich in vitamin C and other active substances such as phenols and flavonoids, which is the very useful for human health.The aim of this study was to investigate the different secondary substances in leaves and fruits (Thompson) in four geographical directions of tree crown. Treatments, including four geographical crown directions (north, south, east and west) and two different organs: leaves and fruit.This study was carried out using the factorial experiment in a completely randomized design with three replications. Biochemical parameters contents of total phenol, total flavonoids, antioxidants, total soluble sugar, Naringin and hesperidin were assessed. The amount of phenols, flavonoids, antioxidant activity and total soluble sugar was measured by spectrophotometer. The amount of Naringin and hesperidin was measured by HPLC.Based on the results, The highest rate of total soluble sugar was observed in the result of interaction of the eastern direction and fruit. The highest rate of Naringin And Hesperidin was observed in the interaction of southern direction and fruit.The highest rate of phenol were observed in interaction of western direction and fruit. The highest rate of flavonoid was in interaction between eastern direction and fruit. The highest total antioxidant activity was in interaction between eastern direction and fruit.according to the results of this experiment, it has been specified that geographical directions of south and east have the maximum effect on the biochemistry characteristics and fruit organ, This may be due to the better and more light received in these parts of the tree canopy.

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