Synthesized Fe3O4 nanoparticles induced antioxidant activity and total phenolic and flavonoid content in Matricaria chamomilla seedlings

Subject Areas : Plant Physiology

Mahsa  Rastegaran ¹ (Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran)
Halimeh  Hassanpour ² (Aerospace Research Institute, Ministry of Science Research and Technology, Tehran 14665-834, Iran)
Hakimeh  Ziyadi ³ (Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Medicinal Sciences, Islamic Azad University, Tehran, Iran)

Received: 2020-12-28 Accepted : 2021-07-18 Published : 2022-01-01

Keywords: nanoparticles, growth, flavonoid, Matricaria chamomilla, phenol,

Abstract :

This study was conducted in order to determine the effects of different concentrations of synthesized Fe3O4 nanoparticles (NPs) on the growth, the content of secondary metabolites and antioxidant capacity in Matricaria chamomilla. With this aim, four levels of Fe3O4 NPs concentrations were applied as follows: basic Murashing and Skoog solution (control), 25, 50 and 100 mg L-1 Fe3O4 NPs. The results indicated that the biomass was higher in the plants that were treated with 25 mg L-1 Fe3O4 NPs than the control plants. Biomass was declined in 50 and 100 mg L-1 Fe3O4-exposed plants compared with the unexposed plants. Relative water content was gradually decreased with the enhancement of Fe3O4 concentration. Fe3O4 NPs in 50 and 100 mg L-1 caused a significant induction in number of root. Fe3O4 NPs treatment enhanced production of secondary metabolites such as total phenol and total flavonoid in roots and leaves of M. chamomilla. Moreover, Fe3O4 NPs increased antioxidant ability of the roots and leaves by inducing DPPH scavenging activity at 25 mg L-1 Fe3O4. The results might identify that the application of Fe3O4 NPs can be a useful war for increasing higher content of secondary metabolites in the M. chamomilla plants.

References:

Ahmadi, N., H. Hassanpour, M. Malak Hekmati, and M. Ghanbarzadeh. 2020. Effect of SiO₂ nanoparticles on phytochemical and anatomical alterations in Anthemis gilanicaꞌ. Iranian Journal of Plant Physiology, 10(3):3223-3231.

Ashouri Sheikhi, A., Hassanpour, H., Jonoubi, P., Nohooji, M.G., Nadimifar, M. 2016. The effect of gamma irradiation on in vitro total phenolic content and antioxidant activity of Ferula gummosa Bioss. J Medic Plant,15(59): 122-131. 

Asif, M. 2012. Phytochemical study of polyphenols in Perilla Frutescens as an antioxidant. Avicenna Journal of Phytomedical, 2:169.

Beyer, S.R., S. Ullrich, S. Kudera, A.T. Gardiner, R.J. Cogdell, and J. Köhler. 2011. Hybrid nanostructures for enhanced light-harvesting: plasmon induced increased in fluorescence from individual photosynthetic pigment-protein complexes. Nano Letter, 11:4897-4901.

Chang, C.C., M.H. Yang, H.M. Wen and J.C. Chern. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3):178-182.

Dimkpa, C.O., J.E. McLean, D.E. Latta, E. Manangón, D.W. Britt, W.P. Johnson, M.I. Boyanov, and A.J. Anderson. 2012. CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand grown wheat. Journal of Nanoparticles Research, 14:1125.

Fageria, N.K. 2016. The use of nutrients in crop plants. Boca Raton: CRC press, pp. 448.

Ghosh, M., S. Bhadra, A. Adegoke, M. Bandyopadhyay, and A. Mukherjee. 2015. MWCNT uptake in Allium cepa root cells induces cytotoxic and genotoxic responses and results in DNA hyper methylation. Mutation Research Fundamntal Moleculare Mech Mutagen, 774:49-58.

Greger, H. 1977. Anthemideae — chemical review. In: Heywood, V.H., Harborne, J.B., Turner, B.L. (eds) The biology and chemistry of the Compositae, vol II. Academic Press, New York, pp 899-941.

Hassanpour, H, and V. Niknam. 2014.Effect of water deficit stress on growth and antioxidant enzyme activity of Mentha pulegium L. at flowering stage. J Plant Proc Func, 3 (8): 25-34.

Hassanpour, H., V. Niknam, and B.S. Haddadi. 2016.High-frequency vibration improve callus growth via antioxidant enzymes induction in Hyoscyamus kurdicus. Plant Cell Tissue Organ Culture (PCTOC),128: 231–241.

Hassanpour, H, and V. Niknam. 2020. Establishment and assessment of cell suspension cultures of Matricaria chamomilla as a possible source of apigenin under static magnetic field. Plant Cell Tissue and Organ Culture, 142(28).

Hassanpour, H, and M. Ghanbarzadeh. 2021. Induction of cell division and antioxidative enzyme activity of Matricaria chamomilla L. cell line under clino-rotation. Plant Cell Tissue Organ Culture (PCTOC). https://link.springer.com/article/10.1007/s11240-021-02060-z.

Hassanpouraghdam, M.B., L.V. Mehrabani, and N. Tzortzakis. 2019. Foliar Application of Nano-zinc and Iron Affects Physiological Attributes of Rosmarinus officinalis and Quietens NaCl Salinity Depression. Journal of Soil Science and Plant Nutrition, 16:1-1.

Khodakovskaya, M.V., K. Desilva, A.S. Biris, E. Dervishi, and H. Villagarcia.  2012. Car­bon nanotubes induce growth enhancement of tobacco cells. In ACS Nano, 6(3):2128-2135.

Krishnaraj, C., E.G. Jagan, R. Ramachandran, S.M. Abirami, N. Mohan, and P.T. Kalaichelvan. 2012. Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. Plant growth metabolism. Process Biochemistry, 47(4):51-658.

Lee, C.W., S. Mahendra, Z. Zodrow, D. Li, Y.C. Tsai, J. Braam, and P.J. Alvarez.2010. Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana. Environmental Toxicology Chemistry: International of Journal, 29(3):669-675.

Ma, X., J. Geiser-Lee, Y. Deng, and A. Kolmakov. 2010.Interactions between engineered nanoparticles (ENPs) and plants: phytotoxicity, uptake and accumulation. Science Total Environment, 408:3053-3061.

Mahmoud, A.W., S.M. Abdelaziz, M.M. El-Mogy, and E.A. Abdeldaym. 2019. Effect of foliar ZnO and FeO nanoparticles application on growth and nutritional quality of red radish and assessment of their accumulation on human health. Agriculture (Pol' nohospodárstvo), 65(1):16-29.

Martínez-Fernández, D., and M. Komárek. 2106.Comparative effects of nanoscale zero-valent iron (nZVI) and Fe₂O₃ nanoparticles on root hydraulic conductivity of Solanum lycopersicum L. Environmental and Experimental Botany, 131:128-36.

Massart, R. 1981.Preparation of aqueous magnetic liquids in alkaline and acidic media. IEEE Trans Magnetics, 17:1247-1248.

Merati, M.J., V. Niknam., H. Hassanpour, and M. Mirmasoumi. 2015. Comparative effects of salt stress on growth and antioxidative responses in different organs of pennyroyal (Mentha pulegium L.). J Plant Res, 28 (5): 1097-1107

Mozafari, A., F. Havas, and N. Ghaderi. 2018. Application of iron nanoparticles and salicylic acid in in vitro culture of strawberries (Fragaria× ananassa Duch.) to cope with drought stress. Plant Cell, Tissue and Organ Culture, 132(3):511-23.

Ramani, S., and S. Kannan. 1985. Studies on Zn up­take and influence of Zn and Fe on chlorophyll de­velopment in young maize cultivars. In Journal of Plant Nutrition, 12:1183-1189.

Rezayian, M, V. Niknam, and H. Ebrahimzadeh. 2018.Improving tolerance against drought in canola by penconazole and calcium. Pesticide biochemistry and physiology, 149:123-36.

Sayadi, V., A.A. Mehrabi, M. Saidi, and K. Nourollahi K. 2014. In vitro culture and callus induction of chamomile (Matricaria chamomilla L.) explants under different concentrations of plant growth regulators. International Journal of Bioscience, (10):206-211.

Shabrangi, A., H. Hassanpour., A. Majd, and M. Sheidai. 2015.Induction of genetic variation by electromagnetic fields in Zea mays L. and Brassica napus L. Caryologia, 68(4): 272-279.

Shankramma, K., S. Yallappa, M.B. Shivanna, and J. Manjanna. 2015. Fe₂O₃ magnetic nanoparticles to enhance S. lycopersicum (tomato) plant growth and their biomineralization. Applied Nanoscience, 6(7):983-90.

Sulaiman, C.T. and I. Balachandran. 2012. Total phenolics and total flavonoids in selected Indian medicinal plants. Indian journal of pharmaceutical sciences, 74(3): 258.

Taghizadeh, M., F. Nasibi, K.M. Kalantari, and F. Ghanati. 2018. Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation. Plant Cell Tissue and Organ Culture, https://doi.org/10.1007/s11240-018-015301.

Tahsili, J., M. Sharifi, N. Safaie, S. Esmaeilzadeh-Bahabadi, and M. Behmanesh. 2014. Induction of lignans and phenolic compounds in cell culture of Linum album by culture filtrate of Fusarium graminearum. Journal of Plant Interaction, 9:412-417.

Whetherley, P.E. 1950. Studies in the water relations of cotton plants. I. The field measurement of water deficit in leaves. New Phytology, 49: 81-87.

Zemestani, M., M. Rafraf, and M. Asghari-Jafarabadi. 2016. Chamomile tea improves glycemic indices and antioxidants status in patients with type 2 diabetes mellitus. Nature, 32:66-72.

Zhang, D.Y., M. Luo, W. Wang, C.J. Zhao, C.B. Gu, Y.G. Zu, Y.J. Fu, X.H. Yao, and M.H. Duan. 2013. Variation of active constituents and antioxidant activity in pyrola (P. incarnata Fisch.) from different sites in Northeast China. Food Chemistry, 141(3):2213-2219.

Zhang, B., L.P. Zheng, W. Yi Li, and J. Wen Wang. 2013. Stimulation of artemisinin production in Artemisia annua hairy roots by Ag-SiO₂ core-shell nanoparticles. Current Nanoscience, 9:363-370