Investigation and comparison of constituent EGCG of tea plant (Camellia sinensis (L.) Kuntze) harvested from the western region of Mazandaran province with callus obtained from tea plant tissue culture by novel method Molecularly Imprinted Polymer (MIP)

Subject Areas : Phytochemistry

Azarm  Movahedi ¹ (Department of chemistry, Faculty of sciences, Gorgan branch, Islamic Azad University, Gorgan, Iran)
Mazyar  Ahmadi Golsefidi ² (Department of chemistry, Faculty of sciences, Gorgan branch, Islamic Azad University, Gorgan, Iran.)
Mehdi  Alizadeh ³ (Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran)

Received: 2020-06-13 Accepted : 2021-03-16 Published : 2021-02-19

Keywords: Callus, tissue culture, Camellia sinensis L, EGCG, MIP,

Abstract :

Due to the importance of constituent Epigallocatechin gallate (EGCG), in the tea plant in use of pharmaceutical and food industries, the amount of EGCG in young leaf, old leaf and callus extract was compared and measured. EGCG in callus extract was isolated and measured by Molecularly Imprinted Polymer (MIP) technique in 1397 in Giah Essence Phytopharm Company. In order to callus induction, callus of tea plant which was harvested from the western region of Mazandaran province cultured in WPM, SH, MS media with different amounts of plant hormones. Leaves and callus extracted with 70% methanol by maceration method. For optimization of extraction and purification, various quantities of functional multiwalled-CNTs 1-10 mg used in production of CNTs-MIP and the performance investigated. EGCG of callus extract extracted with CNTs-MIP and its amount determined with HPLC. The highest volume of callus in WPM medium ,and the highest amount of EGCG in SH medium observed with optimized concentrations of hormones BA(2 mg/l), 2,4_D(0.5 mg/l). The highest content of EGCG was extracted by adding 8 mg of MWCNTs in the polymer structure. EGCG in young and old leaves and callus were 37.34, 7.19 and 0.13 mg/g of dried weight, respectively. By CNTs-MIP, the EGCG content in callus was obtained 0.91 mg/g. Despite the trace amount of EGCG in callus, the amount of this substance can be measured with a concentration coefficient of 8 times by CNTs-MIP, demonstrated the efficiency of this technique.

References:

1.Ahmadi-Golsefidi, M., Es^,haghi, Z., and Sarafraz-Yazdi, A. 2012. Design, synthesis and evaluation of a molecularly imprinted polymer for hollow fiber- solid phase microextraction of chlorogenic acid in medicinal plants. Journal of Chromatography A, 1229: 24-29.

2.Alizadeh, M. 1390. A user manual on Plant tissue culture and micropropagation, Nowruz Publications, Gorgan, 322p.

3. Alothman, Z.A., and Wabaidur, S.M. 2018. Application of carbon nanotubes in extraction and chromatographic analysis: A review. Arabian Journal of Chemistry, 1-19.

4. Bansal, S., Choudhary, S., Sharma, M., Kumar, S.S., Lohan, S., Bhardwaj, V., Syan, N., and Jyoti, S. 2013. Tea: A native source of antimicrobial agents.  Food Research International, 53 (2): 568-584.

5. Basuki, S. 2011. Produksi Epigallocatechin Gallate Pada Kultur in vitro Kalus Camellia sinensis Sebagal Kandidat Pangan Fungsional. Journal Tecknologi Pangan, 5(2):92-100.

6. Beltz, L.A. 2009. The effects of the green tea polyphenol epigallocatechin gallate on the central nervous, endocrine, and innate immune systems. 137-152. In: Ramawat, K.G., (ed.). Herbal Drugs: Ethnomedicine to  Modern Medicine. Springer, India, 402p.

7. Braun, L., Cohen, M., 2015. Herbs & natural supplements, An evidence-based guide. Elsevier, Australia, 1362p.

8. Chopade, V.V., Phatak, A.A., Upaganlawar, A.B., and Tankar, A.A. 2008. Green tea (Camellia sinensis) chemistry, traditional, medicinal uses and its pharmacological activities-a review. Pharmacognosy Reviews, 2 (3): 157-162.

9. Es’haghi, Z., Ahmadi- Golsefidi, M., Saify, A., Tanha, A.A., Rezaeifar, Z., and Alian-Nezhadi, Z. 2010. Carbon nanotube reinforced hollow fiber solid/ liquid phase microextraction: A novel extraction technique for the measurement of caffeic acid in Echinacea purpurea herbal extracts combined with high-performance liquid chromatography. Journal of Chromatography A, 1217: 2768–2775.

10. Es’haghi, Z., Rezaeifar, Z., Rounaghi, G.H., Nezhadi, Z.A., and Ahmadi- Golsefidi, M. 2011. Synthesis and application of a novel solid-phase microextraction adsorbent: hollow fiber supported carbon nanotube reinforced sol-gel for determination of phenobarbital. Analytica Chimica Acta, 689(1): 122-8.

11. Ghasemzadeh, A., and Ghasemzadeh, N. 2011. Flavonoids and phenolic acids: Role and biochemical activity in plants and human. Journal of Medicinal Plants Research, 5(31): 6697-6703.

12.Gruenwald, J., Brendler, T., and Jaenicke, C. 2007. PDR For herbal medicines. Thomson Healthcare Inc, U.S.A, 1026p.

13.Hussain, M.S., Fareed, S., Ansari, S., Rahman, M.A., Ahmad, I.Z., and Saeed, M. 2012. Current approaches toward production of secondary plant metabolites. Journal of Pharmacy and Bioallied Sciences, 4 (1): 10-20.

14.International Standard, 2005, ISO 14502-2(E), Switzerland, 23p.

15.Jain, S.C., Pancholi, B., and Jain, R. 2012. In-vitro Callus Propagation and Secondary Metabolite Quantification in Sericostoma pauciflorum. Iranian Journal of Pharmaceutical Research, 11(4): 1103-1109.

16.Karuppusamy, S. 2009. A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants Research, 3(13): 1222-1239.

17.Khaliq, A., Rashid, H., and Quraishi, A. 2002. Tissue culture studies of Tea (Camellia sinensis L.). Pakistan Journal of Agricultural Research, 17 (3): 297-301.

18.MofidBojnordi, M., Aghdasi, M., Mianabadi, M., and Nadaf, M. 2016. Optimization of callus induction and ephedrine production in Ephedra major. Journal of Plant Researches (Iranian Journal of Biology), 29 (1): 199-209.

19.Muthaiya, M.J., Nagella, P., Thiruvengadam, M., and Mandal, A.K.A. 2013. Enhancement of the Productivity of Tea (Camellia sinensis L.) Secondry Metabolites in Cell Suspension Cultures Using Pathway Inducers. Journal of Crop Science and Biotechnology, 16(2): 143-149.

20.Naz, A., Parween, R., Farheen, R., Kishwar, F., and Anwar, A. 2018. Infusion extracts of commercially available tea bag samples. FUUAST Journal of Biology, 8(1): 147-152.

21.Nikolaeva, T.N., Zagoskina, N.V., and Zaprometov, M.N. 2009. Production of phenolic compounds in callus cultures of tea plant under the effect of 2,4-D and NAA. Russian Journal of Plant Physiology, 56 (1): 45-49.

22.Pizzorno, J.E., Murray, M.T., Joiner-Bey, H. 2016. The clinical^,s handbook of natural medicine. Elsevier Inc, U.S.A, 992p.

23.Ramachandra, R.S., and Ravishankar, G.A. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20: 101–153.

24.Reygaert, W.C. 2014. The antimicrobial possibilities of green tea. Frontiers in Microbiology, 5: 434.

25.Tian, M., Zhang, H., Row, K.H, 2012. Solid- Phase extraction of catechin compounds from green tea by catechin molecular imprinted polymers . Asian Journal of Chemistry. 24 (10): 4606- 4610.

26.Watson, R., and Preedy, V.R. 2008. Botanical medicine in clinical practice. CABI, London, 915p.

27.Xueqing, Y., Yingjun, J., and Nana, Y. 2017. The effective and selective separation of (-) – epigallocatechin gallate. The Journal of Food Science and Technology, 54 (3): 770-777.

28.Zhang, H., Xu, F., Duan, Y.; Luo, X., Zhang, C., Yan, Y., Sun, G., and Sun, X. 2013. Selective adsorption and separation of (-)-epigallocatechin gallate (EGCG) based on silica gel surface molecularly imprinted polymers. IERI Procedia, 5: 339-343.