Essential oil Screening and antioxidant activity of Satureja bachtiarica Bunge. population in Ilam Province for Isolation of Promising Chemotypes

Subject Areas : Phytochemistry

maryam  Mohamad-Zarin Abaadi ¹ (1Former M.Sc. student, Department of Horticulture, College of Agriculture, Ilam University, Ilam, Iran)
Mehdi  Saidi ² (Associate Professor, Department of Horticulture, College of Agriculture, Ilam University, Ilam, Iran)
Yahia  Mohamadi ³ (Assistant Professor, Department of Animal Sciences, College of Agriculture, Ilam University, Ilam, Iran)

Received: 2016-08-25 Accepted : 2017-04-08 Published : 2017-09-11

Keywords: Antioxidant, Ilam, Essential oil, carvacrol, Gilan-e-Gharb, Satureja bachtiarica Bunge, Thym,

Abstract :

This work carried out in order to evaluating of oil yield, phytochemical variation and antioxidant activity of essential oils in different populations of Satureja bachtiarica Bunge. In west of Iran. So the aerial parts of eight wild populations of this plant were collected from different regions of Ilam, Gilane-Gharb provinces and one sample was collected in greenhouse of Ilam University, during 2010 and 2011. The essential oils were obtained by hydro distillation method, were analyzed by GC/MS and the antioxidant activity of them were measured by DPPH assay. The analysis were showed that the populations had significant differences regarding yield, phytochemical composition and their antioxidant activity. Maximum and minimum oil yield were recorded from Shalam and Greenhouse populations, respectively. The main components of plant oil were thymol (39.28-87.30%), p-cymene (0.15-30.63%), γ-terpinene (2.04-14.71%) and carvacrol (0-8.41%). The Shalam population had the highest phytochemical diversity and then the Shalam, Soregeh, KabirKooh and Ghalaarang populations were the most similar populations according to their phytochemical composition. Cluster analysis of populations, which using Ward Algorithm were divided them into three chemotypes. The highest and lowest total phenols contents were recorded in Ghalaarang (292.5±0.91 mg Pyrocatechol/ml) and Gerdakaan (194.04 ±1.08 mg Pyrocatechol/ml) population, respectively. The antioxidant activity of these populations varied from 59.1±0.9 to 73.8±0.45 %  in Green house and Badreh, respectively .The Shalam population had the highest (IC50=0.67 mg/ml) in comparison with synthetic antioxidant BHT (0.11 mg/ml). In cluster B including populations of Soregah, Ghalaarang, Shalam, KabirKooh, Renou, and Badreh were remarked for further domestication processes.  

References:

1. Adams R.P. 1996. Identification of Essential oil components by Gas Choromatography/Mass Spectroscopy. Allured publishing Corp., Carol Stream. USA.


2. Ahmadi, Sh., Sefidcon, F., Baba Khanlou, P., Asgari, F., Khademi, K., Valizade, N. and Karimifar, M.A. 2009. The comparison of existing components in Satureja bachtiarica essence in stages before flowering and full flowering in habitat and farm. Journal of Medicinal and Aromatic Plants Research of Iran, 25(2): 159-169.


3. Amiot, J., Salmon, Y., Collin, C. and Thompson, J.D. 2005. Differential resistance to freezing and spatial distribution in a chemically polymorphic plant Thymus Vulgaris. Ecology Letters, 8: 370-377.


4. Borra, S. K., Gurumurthy, P., Mahendra, J., Jayamathi, K. M., Cherian, C.N. and Chand, R. 2013. Antioxidant and free radical scavenging activity of curcumin determined by using different in vitro and ex vivo models. Journal of Medicinal Plants Research, 7(36): 2680-2690.


5. Dehghan, Z., Sefidkon, F., Emami, S.M.and Kalvandi, R. 2014.The effects of ecological factors on essential oil yield and composition of Ziziphora clinopodioides Lam. Subsp. (rigida Boiss) Rech. f. Journal of Plant Researches (Iranian Journal of Biology), 27(1): 61-71.


6. Fatahi, M. and Fatahi, B. 2010. Principles of medicinal plants. Jahaad-e-Daneshgahi Publications, Tehran Unit, 1^st Edition, Tehran, 486 p.


7. Figueiredo, A.C., Barroso, J.G., Pedro, L.G. and Scheffer, J.C. 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oil. Flavour and Fragerance Journal, 23: 213-226.


8. Kimura, K., Yamaoka, M. and Kamisaka, Y. 2006. Inhibition of lipid accumulation and lipid body formation in oleaginous yeast by effective components in spices, carvacrol, eugenol, thymol and piperine. Journal of Agriculture and Food Chemistry, 54: 3528-3534.


9. Kulkarni, A.P. and Aradhya, S.M. 2005. Chemical changes and antioxidant activity in Pomegranate arils during fruit development. Food Chemistry, 93: 319-324.


10. Mehdi Shahivand, Z., Saidi, M. and, Tahmaasebi, Z. 2013. Effects of plant spacing and irrigation intervals on yield and essential oil percentage of (Satureja bachtiarica Bunge.). Eco-phytochemistry Journal of Medical Plants, 2:25-38. (Abstract in English)


11. Mohamad Nejaad Gandgi, S.M., Moradi, H. Ghanbari, A. and Akbar Zaded, M. 2014. Evaluation of effect of altitude on quantity and quality of essential oil of Rosmarinus officinalis L. cultivated in two region of Maazandaraan provience.  Eco-phytochemical Journal of Medicinal Plants, 2(1): 36-42.


12. Mohamadi Farsani, M., Ghasemi Pirbalouti, A., Bakhshi Khaniki, G. and Momtaz, H. 2016. Effect of paclobutrazol and brassinosteroid elicitors on expression gene 1-deoxy xylulose-5-phosphate reductoisomerase and its relationship with the biosynthesis of monoterpene carvacrol and thymol in Thymus daenenesis Celak under drought stress. Journal of Iranian Plant Ecophysiological Research, 11(43): 76-89.


13. Omidbaigi R. 2005. Production and processing of Medicinal plants. Vol 3, Aastaan Qouds publication, Tehran, 400 p.


14. Omidbeigi, R. and Mahmoodi Sarvestani, M. 2010. Effect of water stress on morphological traits, essential oil content and yield of anise hyssop (Agastache foeniculum [Pursh] Kuntze). Iranian Journal of Horticultural Sciences, 41(2): 153-161. (In Persian)


15. Ou, B., Huang, D., Hampsch-Woodill, M., Flanagan, J.A. and Deemer, E.K. 2002. Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. Journal of Agriculture and Food Chemistry, 50: 3122-3128. 


16. Ruberto, G. and Baratta, M.T. 2000. Antioxidant activity of selected essential oil components in two lipid model system. Food Chemistry, 69: 167-174.


17. Saidi M. 2014. Antioxidant Activities and Chemical Composition of Essential Oils from Satureja khuzestanica, Oliveria decumbens and Thymus daenensis. Journal of Essential Oil Bearing Plants, 17(3): 513-521.


18. Saidi M., Ghafourian, S., Mohamad Zarin-Abaadi, M., Movahedi Kh. and Sadeghifard. N. 2012. In vitro antimicrobial and antioxidant activity of black thyme (Thymbra spicata L.) essential oils. Romanian Archives of Microbiology and Immunology, 71(2): 61-69.


19. Saidi M., Movahedi, Kh. and Mehrabi A.A. 2013. Characterization of genetic diversity in Satureja bachtiarica germplasm in Ilam provience using ISSR and RAPD markers. International Journal of Agriculture & Crop Sciences., 5(17):1934-1940.


20. Saidi M., Movahedi, Kh. Mehrabi A.A. and Kahrizi D. 2013. Molecular genetic diversity of Satureja bakhtiarica. Molecular Biology Reports, 40:6501-6508.


21. Sefidkon F, Jamzad, Z. 2000. Essential oil of Satureja bachtiarica Bunge. Essential Oil Research, 12: 545-55.


22. Sefidkon, F., Jamzade, Z. and Barazande, M., 2004. Satureja bachtiarica Bunge essence as a rich source of Carvacrol, Journal of Medicinal and Aromatic Plants Research of Iran, 20(4): 425-440.


23. Sharaf Zadeh, Sh., Khashkhoie, M. and Javidnia, K. 2008. The effects of nutrient ingredients on growth and effective component of Thymus vulgaris L. Iranian Horticulture Techniques and Sciences Journal, 9(4): 261-274.


24. Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidant activity by mean of Folin-Ciocalteu reagent. Methods Enzymol, 299: 152-178.


25. Trouillas, P., Calliste, C.A. and Allais, D.P. 2003. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the limousine country side asherbal teas. Food Chemistry, 80: 399-407.