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Manuscript ID : EJMP-1708-1271 (R1) Visit : 194 Page: 75 - 86

20.1001.1.23223235.1397.6.1.8.4

Article Type: Original Research

Investigation on variation of alkaloids content in different parts of Physalis divaricata D. Don. From Shahrekord region

Subject Areas : Phytochemistry

Elham Moallem 1 , Prof. Abdollah Ghasemi Pirbalouti 2 , Iraj Mehregan 3 , Taher Nejhad Sattari 4 , Alireza Iranbakhsh 5

1 - Department of Plant Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Research Center for Medicinal Plants, IAU., Shahrekord Branch, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2017-08-15 Accepted : 2018-09-24 Published : 2018-05-22

Keywords: HPLC, atropine, Shahrekord, Physalis divaricate D, Physalin B, Scopolamin,

Abstract :

Physalis divaricata D. Don.is a medicinal plant belonging to Solanaceae family. It consists of different tropane alkaloids, especially atropine, scopolamine, and physalin. In this study, P. divaricata was cultivated at greenhouse conditions in the field experimental of Islamic Azad University of Shahrekord. So various parts of plant, including root, stem, leaf, fruit, and flower at the late flowering stage were harvested in Spring 2015 and then the ethanolic extracts of the samples were obtained by soxhlet extraction method. The contents of atropine, scopolamineand physalin B were measured by calculating the curve of HPLC with three replications. The comparison results at the 5% level indicated that the fruits extract had the highest content of physalin B, the highest content of atropine was reported from the leaves extract and the highest content of scopolamine was reported from the flower extract. In conclusion, the main source of the secondary metabolites seemed to be a difference in type parts of the herb. 

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