بررسی تنوع آلکالوئیدها در اندام‌های مختلف گیاهPhysalis divaricata D. Don. ، شهر کرد

محورهای موضوعی : فیتوشیمی

الهام معلم ¹ , عبدالله قاسمی پیربلوطی ² , ایرج مهرگان ³ , طاهر نژادستاری ⁴ , علیرضا ایرانبخش ⁵

1 - گروه علوم گیاهی دانشگاه علوم تحقیقات تهران
2 - مرکز تحقیقات گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهر کرد
3 - گروه زیست شناسی گیاهی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد علوم تحقیقات تهران، تهران، ایران
4 - گروه زیست شناسی گیاهی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد علوم تحقیقات تهران، تهران، ایران
5 - گروه زیست شناسی گیاهی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد علوم تحقیقات تهران، تهران، ایران

تاریخ دریافت : 1396/05/24 تاریخ پذیرش : 1397/07/02 تاریخ انتشار : 1397/03/01

کلید واژه: آتروپین, اسکوپولامین, کروماتوگرافی مایع با کارآیی بالا, عروسک پشت پرده, فیزالینB,

چکیده مقاله :

عروسک پشت پرده با نام علمی Physalis divaricata D. Don.، گیاهی علفی متعلق به تیره سیب زمینی یا تاج ریزی (Solanaceae) دارای آلکالوئیدهای تروپانی: آتروپین، اسکوپولامین و فیزالین از سکواستروئیدها، می باشد. در این پژوهش، از اندام های مختلف (برگ، ساقه، ریشه، گل و میوه) گیاه عروسک پشت پرده در گل خانه تحقیقاتی دانشگاه آزاد اسلامی واحد شهرکرد و زمان گلدهی در اردیبهشت 1394 برداشت گردید، عصاره اتانولی نمونه‌ها با استفاده از دستگاه سوکسلوله استخراج و به‌ترتیب میزان آتروپین، اسکوپولامین و فیزالین B با استفاده از کرماتوگرافی مایع با کارآیی بالاHPLC ﺑﺎ ﻣﺤﺎﺳﺒﻪ ﺳﻄﺢ زﻳﺮ ﻣﻨﺤﻨﻲ ﻫﺎی و ﻣﻘﺎﻳﺴﻪ آن ها ﺑﺎ ﻣﻨﺤﻨﻲ ﻫﺎی اﺳﺘﺎﻧﺪارد ﺑﺎ ﻣﻴﺎﻧﮕﻴﻦ ﺳﻪ ﺗﻜﺮار ﺑﺮای ﻫﺮﻧﻤﻮﻧﻪ اندازه‌گیری شد. نتایج ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦ در ﺳﻄﺢ 5 درﺻﺪ حاکی از آن است که بیشترین میزان فیزالین، اسکوپولامین و آتروپین، به‌ترتیب در عصاره های میوه، گل و برگ‌های گیاه مشاهده گردید. بررسی یافته‌ها حاکی از تنوع فیتوشیمیایی مواد موثره در اندام های مختلف گیاه بود.

چکیده انگلیسی:

Physalis divaricata D. Don.is a medicinal plant belonging to Solanaceae family. It consists of different tropane alkaloids, especially atropine, scopolamine, and physalin. In this study, P. divaricata was cultivated at greenhouse conditions in the field experimental of Islamic Azad University of Shahrekord. So various parts of plant, including root, stem, leaf, fruit, and flower at the late flowering stage were harvested in Spring 2015 and then the ethanolic extracts of the samples were obtained by soxhlet extraction method. The contents of atropine, scopolamineand physalin B were measured by calculating the curve of HPLC with three replications. The comparison results at the 5% level indicated that the fruits extract had the highest content of physalin B, the highest content of atropine was reported from the leaves extract and the highest content of scopolamine was reported from the flower extract. In conclusion, the main source of the secondary metabolites seemed to be a difference in type parts of the herb. 

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