Phytochemical and antioxidant activity of essential oil in root of Achillea millefolium, Achillea biebersteinii in natural regions of Gilan province

Subject Areas : Phytochemistry

Farshid Rezaei ¹ , Rashid Jamei ²

1 - Department of Biology, Faculty of Science, Urmia University, Iran
2 - Assistant Professor, Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran.

Received: 2017-08-17 Accepted : 2018-02-12 Published : 2018-02-20

Keywords: Essential oil, Flavonoid content, Achillea millefolium, Achillea biebersteinii, Antioxidant &beta, -Thujone,

Abstract :

In recent years, some reports have been presented about the antioxidant and antimicrobial properties of some  root essential oils of  Achillea species. This research carried out in order to evaluating of oil yield, phytochemical variation and antioxidant activity of root essential oils of Achillea millefolium L. and Achillea biebersteinii Afan. These plants were collected in June 2016 from Dogaheh region of Rudbar (1700 m) in Gilan province. The essential oils were obtained by water distillation method (Clevenger apparatus) and were analyzed by (GC/MS) .The phenolic contents were analyzed by high performance liquid chromatography (HPLC),total phenols and flavonoids were determined according to the Folin-Ciocalteu procedure and aluminum chloride colorimetric method, respectively. The antioxidant activity of the essential oils was also evaluated by 1, 1 -diphenyl-2 picrylhydrazyl (DPPH) radical scavenging technique. The findings showed that the main compounds of the A. millefolium essential oil include :β-thujone (18.83%), δ-cadinene (16.87%) and boneol (14.51%), whereas the most abundant compounds in the A. biebersteiniioil were 1,8-cineol (28.82%), p-cimene (15.12%) and camphor (8.34%). According to results, although the total flavonoid content in Achilleamillefolium (54.75 mg/ml oil) was lower than  A.biebersteinii (72.33 mg/ml oil), it had the highest antioxidant activity (74%).

References:

.Abdossi, V. and Kazemi, M. 2016. Bioactivities of Achillea millefolium essential oil   and its main terpenes from Iran. International Journal of Food Properties, 19(8): 1798-1808.

2.Adams, R.P. 2001. Identification of essential oil components by gas    chromatography/mass spectroscopy: Carol Stream, IL Allured Publishing Co. 8(16): 76-81.

3.Bariş, D., Kizil, M., Aytekin, C., Kizil, G., Yavuz, M., Çeken, B. and Ertekin, A. 2011. In vitro antimicrobial and antioxidant activity of ethanol extract of three Hypericum and three Achillea species from Turkey. International Journal of Food Properties, 14: 339–355.

4.Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods. International Journal of Food Microbiolog, 94: 223-253.

5.Djurdjevic, L., Gajic, G., Jaric, S., Kostic, O., Mitrovic, M. and Pavlovic, P. 2013. Analysis of benzoic and cinnamic acid derivatives of some medicinal plants in serbia. Archives of Biological Sciences, 65: 603-609.

6.Eghdami, A. and Sadeghi, F. 2010. Determination of total phenolic and flavonoids contents in methanolic and aqueous extract of Achillea Millefolium. The Organic of Chemistry,2: 81-84.

7.Figueroa, F., Marhuenda, J., Cerda, B., Zafrilla, P., Martínez-Cacha, A., Tejada, L., Villano, D. and Mulero, J. 2016. HPLC-DAD Determination and availability of phenolic compounds in 10 genotypes of walnuts. International Journal of Food Properties, 20(3):1-33.

8.Ghani, A., Azizi, M., Hassanzadeh-Khayyat, M. and Pahlavanpour, A.A. 2008.    Essential oil composition of  Achillea eriophora,  A. nobilis, A. biebersteinii and A. wilhelmsii from Iran. Journal of Essential Oil Bearing Plants, 11(5): 460-467.

9.Gharibi, S., Tabatabaei, B. and Saeidi, G. 2015. Comparison of essential oil composition, flavonoid  content and antioxidant activity in eight Achillea species. Journal of Essential Oil Bearing Plants, 18(6): 1382-1394.

10.Kahkonen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K. and Kujala, T.S. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47(10): 3954-3962.

11.Kheiry, A., Sefidkon, F., Delshad, M., Fattahi Moghaddam, M.R. and Izadi, A. 2012.Phytochemical variation of essential oils of Achillea millefolium L. from different   habitats of Iran. Iranian Journal of Medicinal and Aromatic Plants, 28 (4):767-779.

12.Kleinwachter, M. and Selmar, D. 2015. New insights explain that drought stress
enhances the quality of spice and medicinal plants: potential applications.
Agronomy for Sustainable Development, 35(1): 121-131.

13.Lazarevic, J., Radulovic, N., Zlatkovic, B. and Palic, R. 2010. Composition of Achillea distans Willd. subsp. distans root essential oil. Natural Product Research, 24 (8): 718-731.

14.Mockute, D. and Judzentiene, A. 2003. Variability of the essential oils composition of Achillea millefolium ssp. millefolium growing wild in Lithuania. Biochemical     Systematics and Ecology, 31(9):1033-1045.

15.Mozaffarian V. 2009. A Dictionary of Iranian Plant Names, Farhang Moaser   Publishers. Tehran, Iran.

16.Polatoglua, K., Karakoc, O.C. and Goren, N. 2013. Phytotoxic, DPPH scavenging, insecticidal activities and essential oil composition of Achillea vermicularis, A .teretifolia and proposed chemotypes of A. biebersteinii (Asteraceae). Industrial Crops and Products, 51(1): 35- 45.

   17.Rahimmalek, M., Sayed-Tabatabaei, B.E., Etemadi, N., Goli, S.A.H., Arzani, A. and Zeinali, H. 2009. Assessment of genetic diversity among and within Achillea species using amplified fragment length polymorphism (AFLP). Biochemical Systematics and Ecology, 37(4): 354-361.

 18. Rezaei, F., Jamei, R., Heidari, R. and Maleki, R. 2017. Chemical composition and antioxidant activity of oil from wild Achillea setacea and A. vermicularis. Intenational Journal of Food Properties, 20(7): 1522-1531.

19.Saeidnia, S., Gohari, A.R., Mokhber-Dezfuli, N. and Kiuchi, F. 2011. A Review on Phytochemistry and medicinal properties of the genus Achillea. DARU Journal of Pharmaceutical Sciences,19(3): 173-186. 

20.Shin, Y., Liu, R.H., Nock, J.F., Holliday, D. and Watkins, C.B. 2007. Temperature and relative humidity effects on quality, total ascorbic acid, phenolic and flavonoid concentrations, and antioxidant activity of strawberry. Postharvest Biology and Technology, 45(3):349-357.

21.Turkmenoglu, F.P., Tuncay, O., Akaydin, G., Hayran, M. and Demirci, B. 2015. Characterization of volatile compounds of eleven Achillea species from Turkey and biological activities of essential oil and methanol extract of A. hamzaoglui Arabac & Budak. Molecules, 20(6): 11432-458.

22.Zijia, Z., Liping, L., Jeffrey, M., Tao, W. and Zhengtao, W. 2009. Antioxidant phenolic compounds from walnut kernels (Juglans regia L.). Food Chemistry, 113: 160-165.