Investigation of secondary metabolites of Dorema kopetdaghense Pimenov in Khorasan Razavi province
Subject Areas : Phytochemistryzahra mohamadzade shabestari 1 * , Raheleh Rahbarian 2
1 - Department of Biology, Payam Noor University, Tehran, Iran
2 - Department of Biology, Payame Noor University, Iran
Keywords: Dorema, Dorema kopetdaghense, Endemic Khorasan, Phytochemistry,
Abstract :
The Dorema genus belongs to the Apiaceae family, which includes aromatic and medicinal species. Many chemical compounds, including terpenes, coumarins and phenolic compounds, have been isolated from Dorema species, and a wide range of antimicrobial, anti-inflammatory, antioxidant, cytotoxic, etc. activities have been reported from this genus in modern medicine. Since the species of this genus have valuable medicinal properties, it is possible that D. kopetdaghense Pimenov is a valuable medicinal plant whose properties remain almost unknown, and limited studies have been done on its compounds and secondary metabolites. In this study, the chemical compounds present in the extract of shoot and root were studied separately. In this study, the plant was collected in spring and after drying, hydroalcoholic extract from the aerial parts and roots of the plant was prepared by Soxhlet extraction and the essential oil of the plant was prepared by Clevenger apparatus. Then, phenolic compounds, anthocyanins and fatty acids were measured by the HPLC method, alkaloids by LC-MS method and terpenoids by GC-MS method. Four phenolic compounds, rutin, quercetin, quercitrin, and luteolin were identified in the essential oil of the shoot and root of D. kopetdaghense Pimenov in shoot and root. 3 phenolic compounds, myricetin, m-coumaric acid and apigenin were found only in the aerial parts of the plant. 3 fatty acids campesterol, stigmasterol and beta-sitosterol were identified in shoot and root. 3 alkaloidal compounds cocsuline, berbamin and dauricine were reported in shoot and root. The most common compound identified in the plant was terpenoid content, which was reported as 38 compounds in aerial parts and 32 compounds in roots. Limonene, caryophyllene oxide, phytol, farnesol and neophytadine are the main terpene compounds identified in the plant.
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