Subject Areas : Medicinal plants

عالمه سرایانی ¹ , ندا امینی ^{2 *}

1 - موسسه آموزش عالی کاویان
2 - موسسه آموزش عالی کاویان

Received: 2024-01-09 Accepted : 2024-02-15 Published : 2024-02-15

Keywords: Antioxidant property, Ethanol extract, Glycerol, Helichrysum oocephalum Boiss plant, Propylene glycol,

Abstract :

Harmful chemicals, exposure to various types of radiation, and lifestyle changes have disrupted the oxidative balance in the body, as a result of which the amount of free radicals has increased, and this increase has a direct role in causing various diseases such as cancer. Therefore, it is very necessary to identify compounds with antioxidant properties, since plants are rich in antioxidant compounds and are available to people, so the present study was conducted with the aim of investigating the antioxidant properties of Helichrysum oocephalum Boiss plant extract. In this research, after collecting the plant, its aerial parts and roots were powdered, then extraction was done by maceration method. For this purpose, three different solvents (propylene glycol, ethanol and glycerol) were used and the concentrations were 2.5, 5, 10, 20 and 40 mg/ml were obtained from propylene glycol, ethanol and glycerol extracts. Finally, the antioxidant properties of the extracts were measured using the DPPH test. Using the IC50 test, the concentration of the extract, which has antioxidant properties, was obtained. The results showed that the propylene glycol extract of the plant at a concentration of 40 mg/ml has the highest antioxidant properties, while the glycerol and ethanol extracts were ranked next. Statistical analysis also showed a significant difference (P<0.05). Also, the IC50 value for propylene glycol extract is equal to 12.40, which shows the high antioxidant power of the desired extract compared to the control, and the ethanol and glycerol extracts are after the propylene glycol extract, respectively. It seems that the propylene glycol extract of the plant at a concentration of 40 mg/ml can be used as an antioxidant compound in various industries including pharmaceuticals.

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