Exopolysaccharides production from lactobacill isolated from Lighvan Kashk
Subject Areas : Biotechnology and Applied Microbiology journal
Keywords: Prebiotic, Exopolysaccharides (EPS), Lactic acid bacterium (LAB), Kashk,
Abstract :
Exopolysaccharides (EPS) are high molecular weight polymers which are long chain composed of sugar residues and secreted by microorganisms into the surrounding environment. On the other faceLAB EPS have been claimed to have antitumor effects, immunostimulatory activity, and to lower blood cholesterol Therefore, EPS from LAB havepotential for development and exploitation as food additives or functional food ingredients with both health and economic benefits. Kashk is popular fermented milk product of Iran consumed in almost every house hold. Kashk is the primary protein source of the nomadic people of Iran. In this study we tried to characterize native LABs who produce EPS in large amount. Methods: This study is based on measurement of EPS-b & EPS-r production of approximately 23 different isolated LABs kashk of Iran. 37◦ C, 5% Co2 or anaerobic condition and 98% moisture_ used medium was MRS (Merck, Germany). Then, Colonies were addressed for both microscopy and macroscopy features. To measurement produced EPS-b & EPS-r, we supplied phenol/sulfuric acid method. Results analysis is founded upon glucose standard curve. Results: The EPS production of the two strains was comparable, ranging from66.6 to 25.157 mg/l for EPS-b (2 species among 23), while this domain is 1134.4 to 367 mg/l for EPS-r (2 species among 30). Conclusions: Infood industry, microbial EPS are used as thickeners or viscosifiers, stabilizing or emulsifying agents or texturizers.
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