Investigation of genetic diversity in 7 subcultures of siokra cultivar

Subject Areas : Biotechnology and Applied Microbiology journal

Received: 2012-11-04 Accepted : 2017-11-05 Published : 2013-02-19

Keywords: tissue culture, RAPD marker, Gossypium hirsotum L, Zeatin,

Abstract :

Tetraploid Cotton (Gossypium hirsutum L.) is considered as one of the most important crop plants in Iran, cultivated in various regions of the country. Continuous cultivation of the same genotypes may bring about genetic erosion in the long term; therefore study of the available genetic variability as well as introducing the new ones is of importance. One of the appropriate techniques to make changes and useful genetic diversity are tissue culture technique and creation of somaclonal variation. The best treatment for multiple shoot induction contained Murashig and Skoog (MS) basal medium supplemented with Zeatin (0.1 mgl‾¹) and activated charcoal (0.5 mgl‾¹) was optimized for Sahel cultivar. RAPD markers were used for detection of somaclonal variation in tissue culture. RAPD analysis revealed significant molecular difference between parental genotypes and regenerated plants of different sub-cultures. The cultivars differed in the level of genetic diversity produced due to somaclonal variation which seems to be genotype dependent.