Assessment of culture media for growth of Lactobacillus as a probiotic bacteria in the milk products

Subject Areas : Biotechnology and Applied Microbiology journal

Received: 2017-11-05 Accepted : 2017-11-05 Published : 2013-05-22

Keywords: Probiotic, Lactobacillus, culture media,

Abstract :

This study has focused on evaluation of five culture media, and four different incubation conditions to assess their suitability for achiving the best recovery of Lactobacillus acidophilus. Bacteriological media evaluated included; M17 agar, MRS agar, MRS-bile agar, and acidified MRS agar (pH;5.20). Incubation was carried out under aerobic and anaerobic conditions at 37°C and 45°C for 72h. The results, show the overall suitability for 37°C temperature, and the best recovery is obtained for MRS agar, under anaerobic condition. But there is no growth on the acidified MRS agar. The interesting growth of L.acidophilus on the MRS-bile agar, under anaerobic condition and 37°C, shows the highly resistance of this bacteria to the bile acids.