Based on healthy properties of gelatin, strengthening it for using as multi functional material is essential. In this paper nano scaffold of gelatin was doped with nano zinc oxide (ZnO) (with different percent) and electrospun. The morphology of obtained nano scaffold was study by Field Emission Scanning Electron Microscope (F E S E M) and elemental mapping proved the present and distribution of nano zinc oxide (ZnO) in scaffold. Also, X ray Fluorescence (XRF) test was done to confirm the amount of used nano material. The anti bacterial property of the obtained scaffold was investigated against both gram negative and gram positive bacteria (Shigella and Staphylococcus Aureus respectively) and the results show that the nano scaffold has more than 96% anti bacterial property. On the other hands, the anti inflammatory effect of nano scaffold was investigated on rats and the results illustrated that nano scaffold is effective for edema decrease. Manuscript profile