Evaluation of total phenol and flavonoid content, antioxidant activity, anti-pain and anti-inflammatory of Ocimum basillicum var. purpurescens in comparison with opioid drug (morphine) in male rats

Subject Areas : Medicinal Plants

Solmaz Fakhari ¹ , Isa bilejani ² , Arash Khaki ³ , Asghar Rajabzadeh ⁴ , Masoumeh Mazandarani ⁵

1 - Department of Anesthesiology, Imam Reza Hospital, Tabriz University of Medical Sciences, , Iran
2 - 3Department of Anesthesiology, Shahid Madani Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
3 - Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 - 2Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
5 - 5Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran

Received: 2017-02-20 Accepted : 2017-04-30 Published : 2017-05-22

Keywords: Phenol, Flavonoid, Antioxidant, Pain, Ocimum basilicum, SOD activity, Opioid drugs (morphine),

Abstract :

This study was carried out to evaluate the total flavonoide, phenols, antioxidant, anti-pain and anti-inflammatory in different extracts of Ocimum ebasillicum var. purpurescens in comparison with opioid drug (morphine) in male rats. The male Wistar rats with 250±10 g weighing was used and randomly divided to five groups. Group I (treated by 0.1mg/kg acetic acid, II-IV (treated by 0.7, 0.9 and 1 mg/kgof plantextract) , V (treated by 0.1 mg/kg morphine) and evaluated by Writhing test. The total phenol and flavonoids content was evaluated by follin-ciocalteus reagent, Ammonium chloride, the antioxidant activity was measured by DPPH method  and  serum levels of PGE2 and SOD activity measured by ELISA Kits.  According to results, the ethanol extract of plant had more content of secondary metabolites with total phenol (288.3±51.5 mg EGA/g) content, flavonoide content (154.7±1.04mg EGU/g) and the highest ability of free radicals scavenging (79.4±4.1%). There was a significant correlation between phenol- flavonoid ingredients to antioxidant activity rate and free radicals scavenging of plant (P<0.05, r<0.9) extract. High dosages of plant extract could be decreased chronic pains, in addition, serum level of PGE2 has been showed a significantly decreasing in groups treated by Ocimum basilicum extract and morphine in compared to untreated group. The serum SOD of treated groups (plant extract) was observed near to morphine group (P<0.05). Anti-inflammatory and anti-pain effects of this plant statically were observed next to morphine. It is found that steadily consumption of herbal usage, as antioxidant and anti-inflammatory, could be medicated to inflammatory treatment and long-term pains instead of opioid drugs.

References:

1. Alessandra, C.P., Fernanda M., Daniel G. and Neiva, D.R. 2016. Extraction of bioactive compounds and free radical scavenging activity of purple basil (Ocimum basilicum L.) leaf extracts as affected by temperature and time. (Annals of the Brazilian Academy of Sciences). Printed version ISSN: 0001-3765 / Online version ISSN: 1678-2690. 


2. Al-Hashimi, A.G. 2012. Antioxidant and antibacterial activities of Hibiscus sabdariffa L. extracts. African Journal of Food Science, 6(21): 506-511.


3. Azizian Shermeh, O., Valizadeh, J., Noroozifar, M. and Qasemi, A. 2016. Investigating the antimicrobial activities of silver nanoparticles biosynthesized by aqueous extract of Sambucus ebulus L.Journal of Ilam University of Medical Sciences. 25(4): 92-108. (In Persian).


4. Barchan, A., Bakkali, M., Arakrak, A., Pagán, R. and Laglaoui, A. 2014. The effects of sol‑vents polaritiy on the phenolic contents and antioxidant activity of three Mentha species extracts. International Journal of Current Microbiology and Applied Sciences, 3(11): 399-412.


5. Blednov, Y.A., Stoffel, M., Alva, H. and Harris, R.A. 2003. A pervasive mechanism for analgesia: Activation of GIRK2 channels.  Molecular Pain, 1: 277–82.


6. Collier, H.O.J., Dinneen L.C., Johnson C.A. and Schneider, C. 1968. The abdominal constriction response and its suppression by analgesic drugs in the mouse. British Journal Pharmacol., 32: 295–310.


7. Chang, C., Yang, M., Wen, H. and Chern, J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis, 10: 178-182.


8. Carvalho-Filho, J.L.S., Blank, A.F., Alves, P.B., Ehlert, P.b.a., Melo, A.S., Cavalcanti, S.C.H., Arrigoni-Blank ,M.F. and Silva-Mann, R. 2006. Influence of the harvesting time, temperature and drying period on basil (Ocimum basilicum L.) essential oil. Revista Brasileira de Farmacognosia 16: 24-30.


9. Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I. and Heinrich, M. 2014. Hibiscus sabdariffa L.– A phytochemical and pharmacological review. Food Chemistry. 165: 424–443.


10. Dinanath, D Patil., Dnyandeo, K. Mhaske., Gurumeet C. Wadhawa. 2011. Antibacterial and antioxidant study of Ocimum basilicum Labiatae (sweetbasil). Journal of Advanced Pharmacy Education and Research 2: 104-112.


11. Durak, I., Yurtarslanl, Z., Canbolat, O. and Akyol, O. 1993. A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clinica Chimica Acta, 214: 103-4.


12. Ebrahimzadeh, M.A., Pourmorad, F. and Hafezi, S. 2008. Antioxidant activities of Iranian corn silk. Turkish Journal of Biology, 32: 43-49.


13. Ekhtiari, H., Behzadi, A., Sadeqi, M., Mirbaha, H., Nowroozi, L. and Alavi, A. 2002. Recognition and treatment of addiction. 1st ed. Tehran: Arjomand Publishing Co. 14-31.


14. Fields, H. 2004.State-dependent opioid control of pain. Nature Reviews Neuroscience, 5: 565-75.


15. Gioiosa, L., Chen, X., Watkins, R., Elizabeth, A., Umeda, A. and Arnold, P. 2008. Sex chromosome complement affects nociception and analgesia in Newborn mice. Journal Pain, 10: 962-9.


16. Goldman, L. and Bennett, J.C. 2000. Cecil textbook of medicine. 21th ed. WB Saunders Co, P. 103.


17. Goudet, C., Magnaghi, V., Landry, M., Nagy, F. and Gereau, R.W. 2009. Metabotropic receptors for glutamate and GABA in pain. Brain Res. Rev., 60: 43-56.


18. Guiche, R.E.L., Tahrouch, S., Amri, O., Mehrach, K.E.L. and Hatimie, A. 2015. Antioxidant activity and total phenolic and flavonoid contents of 30 medicinal and aromatic, plants located in the south of morocco, International Journal of New Technology and Research, 1(3): 7-11.


19. Hossaini, M., Duraku, L.S., Saraē, E. and Jongen, J.L.M. 2010. Differential distribution of activated spinal neurons containing glycine and/ or GABA and expressing c-fos in acute and chronic pain models. Pain, 151: 356-65


20. Jamshidi, M., Ahmadi, H.R., Rezazadeh, S., Fathi, F. and Mazanderani, M. 2010. Study on phenolic and antioxidant activity of some selected plant of Mazandaran province. Medicinal Plant, 9(34): 177-183.


21. Kamkar, A., Asadi, F., Jenneli-javan, A. and Jamshidi, R. 2009. Antioxidant capacity of essential oil and extract of Iranian Mentha spicat. Journal of Veterinary Medicin and Labarotory, 1(1): 69-77.


22. Khaki, A., Fatemeh F.A., Mohamad, N. and Amir Afshin, Kh. 2011. Effects of basil, Ocimum basilicum on spermatogenesis in rats. Journal of Medicinal Plants Research, 5(18): 4601-4604.


23. Khorasani Esmaeili, A., Taha, R.M., Mohajer, S. and Banisalam, B. 2016. Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pratense L. (Red Clover). BioMed Research International, 1-11.


24. Kim, S.J., Kim, J.M., Shim, S.H. and Chang, H.I. 2014. Anthocyanins accelerate the healing of naproxen-induced gastric ulcer in rats by activating antioxidant enzymes via modulation of Nrf2. Journal Funct. Foods 7: 569-579.


25. Kordi tamandani, E., Valizadeh, J. and Valizadeh, M. 2014. In vitro production of secondary metabolites in Cicerspiroceras using elicitors. Global Journal of Research on Medicinal Plants and Indigenous Medicine, 3(2):48-56.


26. Lee, S.J., Umano ,K., Shibamoto, T. and Lee, K.G. 2005. Identification of volatile components in basil (Ocimum baslicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chem., 91: 131-137.


27. Mathew, S. and Abraham, T.E. 2006. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology, 44: 198-206.


28. Mohamed, S.A., and Khan, J.A. 2014. antioxidant capacity of chewing stick miswak (Salvadora persica), BMC. Complementary and Alternative Medicine, 13(40): 1-6.


29. Mohajerani, M. 2012. Antioxidant activity and total phenolic content of Nerium oleander L. grown in North of Iran, Iranian Journal of Pharmaceutical Research, 11(4): 1121-1126.


30. Moure, A., Cruz, J.M., Franco, D., Dominguez, J.M., Sineiro, J., Dominguez, H., Nunez, M.J. and Parajo, J.C. 2001. Natural antioxidants from residual sources. Food Chemistry. 72(2):145-171.


31. Norhaizan, M.E., Tong, S.H., Amin, I. and Chew, L.Y. 2010. Antioxidant activity in different parts of the Roselle (Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds. Food Chemistry, 122: 1055-1060.


32. Nouri, S., Kiasat, A.R., Kolahi. M., Mirzajani, R. and Seyednejad, S.M. 2016. Phytochemical studies, antioxidants and various optimization methods in order to determine the best method of extracting curcumin extract ethanol from the plant Curcuma longa L. Eco-phytochemical Journal of Medicinal Plants, 11(3): 1-11. (In Persian).


33. Nguyen, P.M., Kwee, E.M. and Niemeyer, E.D. 2010. Potassium rate alters the antioxidante capacity and phenolic concentration of basil (Ocimum basilicum L.) leaves. Food Chem. 123: 1235-1241.


34. Ombra, M.N., Fratianni, F., Granese, T., Cardinale, F., Cozzolino, A. and Nazzaro, F. 2015. In vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (Solanum tuberosum cv. Vitelotte noire) following simulated gastrointestinal digestion. Natural Product Research. 29: 1087-1091.


35. Politeo, O., Jukic, M. and Milos, M. 2007. Chemical composition and antioxidant capacity of free volatile aglycones from basil (Ocimum basilicum L.) compared with its essential oil. Food Chemistry, 101: 379-385.


36. Rang, H.P., Dale, M.M. and Ritter, J.M. 1999. Text book of pharmacology. New York: Churchill Livingston, 3:148-633.


37. Ricciotti E. and Fitzgerald, G.A. 2011. Prostaglandins and inflammation. Arterioscler. Thromb. Vasc. Biol., 31(5): 986-1000.


38. Sadeghi, Z., Valizadeh, J. and Azizian Shermeh, O. 2015 (a). Antioxidant activity and total phenolic content of some date varieties from Saravan Region, Baluchistan, Iran. Journal of a Medicinal Plants Research, 9(4): 78-83.


39. Sadeghi, Z., Valizadeh, J., Azizian Shermeh, O. and Akaberi, M. 2015 (c). Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchistan, Iran. Avicenna Journal of Phytomededicine, 5(1): 1-9.


40. Sajjadi, S.E. 2006. Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran. DARU Journal of Pharmaceutical Sciences, 14(3):128-130.


41. Sirag, N., Elhadi, M.M., Algaili, M., Algaili, M., Hozeifa, M.H. and Ohaj, M. 2014. Determination of total phenolic content and antioxidant activity of Roselle (Hibiscus sabdariffa L.) Calyx ethanolic extract. Standard Research Journal of Pharmacy and Pharmacology, 1(2): 34-39.


42. Sun, Y., Oberley, L.W. and Li, Y. 1988. A simple method for clinical assay of superoxide dismutase, Clinical Chemistry., 34: 497-500.


43. Szy manowska, U., Złotek, U., Karaś, M. and Baraniak, B. 2015. Anti-inflammatory and antioxidative activity of anthocyanins from purple basil leaves induced by selected abiotic elicitors. Food Chem. 172: 71-77.


44. Tabart, J., Kevers, C., Pincemail, J., Ddefraigne, J.O. and Dommes, J. 2008. Comparative antioxidant capacities of phenolic compounds measured by various tests. Journal of Food Chemistry, 113: 1226-1233.


45. Tzulker, R., Glazer, I., Bar-Ilan, I., Holland, D., Aviram, M. and Amir, R. 2007. Antioxidant activeity, polyphenol content and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. Journal Agricultural Food Chemistry, 55: 9559-7.


46. Usman, L.A., Ismaeel, R.O., Zubair, M.F., Saliu, B.K., Olawore, N.O. and Elelu, N. 2013. Comparative studies of constituents and antibacterial activities of leaf and fruit essential oils of Ocimium basilicum grown in north central Nigeria. International Journal of Chemical and Biochemical Sciences. 3: 47-52.


47. Wall, P.D. and Melozoc R. 1991. Text boock of pain. Second edit Churchill Livingstone, P. 1.


48. Weiner, R.S. 2001. Pain management. 6th ed. American Academy of pain management, P: 3-9.


49. Zhang, M., Hettiarachchy, N.S., Horax, R., Kannan, A., Praisoody, M.D.A, Muhundan, A. and Mallangi, C.R. 2011. Phytochemicals, antioxidant and antimicrobial activity of Hibiscus sabdariffa, Centella asiatica, Moringaoleifera and Murraya koenigii leaves. Journal of Medicinal Plants Research, 5(30): 6672-6680.


50. Zafra-Stone, S., Yasmin, T., Baqchi, M., Chatterjee, A., Vinson, J.A. and Baqchi D. 2007. Berry anthocyanin’s as novel antioxidants in humen health and diseases prevention Molecular Nutrition and Food Research., 51(6): 675-83.