Evaluation of antioxidant activity of endophytic fungi isolated from some native medicinal species of Golestan province

Subject Areas : Mycology

sareh hatamzadeh ¹ , kamran Rahnama ² , saeed nasrollahnejad ³ , khalil-berdi fotohifar ⁴ , khodayar hemmati ⁵ , james white ⁶

1 - Department of Plant Protection, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan. Iran
2 - Department of Plant Protection,Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan. Iran
3 - Department of Plant Protection, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan. Iran
4 - Department of Plant Protection, Faculty of Agricultural sciences and Natural resources, Tehran University, Karaj, Iran
5 - Department of Horticulture, Faculty of plant production, Gorgan University of Agricultural sciences and Natural Resources, Gorgan. Iran
6 - Department of Biology and plant pathology, Rutgers University of New Jersey, New Brunswick, US

Received: 2018-11-10 Accepted : 2019-05-21 Published : 2020-05-21

Keywords: Medicinal Plants, antioxidant activity, Endophytic Fungi, Asreraceae family,

Abstract :

Medicinal plants are a very rich source of antioxidant compounds. Endophytic fungi of medicinal plants, due to long-term coexistence with these plants produce plant secondary metabolites. Therefore, in this study, the antioxidant properties of endophytic fungi isolated from 7 medicinal plants of the Asteraceae family includeing Matricaria chamomilla, Anthemis triumfetii, Anthemis parthenium, Anthemis altissima var. Altissima, Achillea millefolium, Achillea filipendulina, Cichorium intybus was investigated. The samplings were done from healthy plants and free of any diseases from most areas of Golestan province during 2016 spring. After morphological and molecular identification of endophytic fungi, the antioxidant property of 37 species of endophytic fungi was evaluated by DPPH free radicals metod. Based on the results, a significant difference of 99% was observed between the antioxidant properties of endophytic fungi. The lowest (32.1% ) and highest (98.8%) antioxidant activity were related to the Stemphylium amaranthi and Trametes versicolor  fungi isolated from Anthemis triumfetii leaf and Achillea santolina stem tissues, respectively. In addition, the Schizophyllum commune with 98.8% antioxidant activity was placed in the same group with T. versicolor. The Cladioporium spp. such as Cladosporium cladosporioides and Cladosporium ramotenelum showed a high antioxidant activity of about 97%. Considering short-term production and the high growth rate of fungi, endophytes maybe a good choice for the production of antioxidant substances.

References:

1. Aly, A.H., Debbab, A. and Chaidir, C. 2011. Fungal endophytes: Unique plant inhabitants with great promises. Applied Microbiology and Biotechnology, 90(6):1829-45.


2. Arbaayah, H.H. and Umi K.Y. 2013. Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and split gill mushroom (Schizophyllum commune) ethanolic extracts. Mycosphere, 4 (4): 661-673.


3. Arona, M.B., Cano, A. and Acosta, M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry, 73: 239-244.


4. Barros, L., Ferreira, M.J., Queirós, B., Ferreira, C.F.R. and Baptista, P. 2007. Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry, 103: 413-419.


5. Barnett, H.L. and Hunter, B.B. 1999. Illustrated genera of imperfect fungi. APS press, 217pp.


6. D'Amico, M., Frisullo S. and Cirulli M. 2008. Endophytic fungi occurring in fennel, lettuce, chicory, and celery−commercial crops in southern Italy. Mycological Research, 112: 100-107.


7. Duan, X.J., Zhang, W.W., Li, X.M. and Wang, B.G. 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chemistry, 95: 37-43.


8. Ebrahimzadeh, M.A., Hosseinimehr, S.J., Hamidinia, A. and Jafari, M. 2008. Antioxidant, and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology, 1: 7-14.


9. Ellis, M.B. 1997. Dematiaceous Hyphomycetes. Commonw. Mycol. Inst. Kew, Surrey, England, 608pp.


10. Georgieva, L., Gadjalova, A., Mihaylova, D. and Pavlov, A. 2015. Achillea millefolium L. – phytochemical profile and in vitro antioxidant activity. International Food Research Journal, 22(4): 1347-1352.


11. Guo, L.D. 1999. Identification of endophytic fungi in Livistona chinesis (Palmae). Ph. D dissertation, Department of Ecology and Biodiversity, University of Hong Kong. 243pp.


12. Guo, B. 2000. Cytonic acids A & B: novel tridepside inhibitors of hCMV protease from the endophytic fungus Cytonaema species. Journal of Natural Product, 63: 602-604.


13. Hatamzadeh, M., Rahnama, K., Nasrollahnejad, S., Fotowhifar, K., Hemmati, Kh. and White, J. 2017. Isolation and Identification of some endophytic fungi of four species of Camomille in Golestan province. 3rd Iranian Mycological Congress, Kurdistan, Sanandaj, Iran.


14. Hatamzadeh, M., Rahnama, K., Nasrollahnejad, S., Fotowhifar, K., Hemmati, Kh. and White. J. 2017. Effect of plant tissue and culture media on the isolation rate of endophytic fungi of some medicinal plants. 3rd Iranian Mycological Congress, Kurdistan, Sanandaj, Iran.


15. Hulikere, M.M., Joshi, G.C.D., Jagadeesh J. and T. Nivya. 2016. Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii). Mycology, 20(40): 22-29.


16. Jagadish, L.K., Krishnan, V.V., Shenbhagaraman, R. and Kaviyarasan, V. 2009. Comparitive study on the antioxidant, anticancer and antimicrobial property of Agaricus bisporus (J.E. Lange) Imbach before and after boiling. African Journal of Biotechnology, 8(4): 654-661.


17. Jam Ashkezari, S., Fotouhifar, K. and Farzaneh, M. 2014. Identification of some endophytic fungi of common yew trees (Taxus baccata) in Iran. Rostaniha, 15(1): 50-64.


18. Jhan, M.H., Yeh, C.H., Tsai, C.C., Kao, C.T., Chang, C.K. and Hsieh, C.W. 2016. Enhancing the antioxidant ability of Trametes versicolor polysaccharopeptides by an enzymatic hydrolysis process. Molecules. 21: 215-220.


19. Leong, L.P. and Shui, G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chemistry, 76: 69-75.


20. Jagadish, L.K., Krishnan, V.V., Shenbhagaraman, R. and Kaviyarasan, V. 2009. Comparative study on the antioxidant, anticancer and antimicrobial property of Agaricus bisporus imbach before and after boiling. African Journal of Biotechnology, 8: 654-661. Liu, X., Mingsheng D., Xiaohong Ch., Mei J., Xin L.V. and Guijun, Y. 2007. Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba. Food Chemistry, 105: 548-554.


21. Masoumi, S., Mirzai, S., Kalvandi. R. and Zafari D. 2012. Identification of fungal endophytes of thyme in Hamedan province. 20th Iranian Plant Protection Congress, Shiraz, Iran, 127.


22. Mirfat, A.H.S., Noorlidah A. and Vikineswary, S. 2010. Scavenging activity of Schizophyllum commune extracts and its correlation to total phenolic content. Journal of tropical agriculture and food science, 38(2): 231-238.


23. Mirzaei, A., Akbartabartori, M., Sadeghi, H. and Sharifi B. 2010. The evaluation of total phenol and antioxidant activity yarrow, wormwood and chamomile. Journal of Armaghane Danesh. 15: 243-252.


24. Natarajan, K. and Kolandavelu K. 1998. Resupinate Aphyllophorales of Tamil Nadu, India. Centre for advanced study in Botany, University of Madras, 133pp.


25. Raja, H.A., Miller, A.N., Pearce, C.J. and Oberlies, N.H. 2017.  Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. Journal of Natural Product, 80(3): 756-770.


26. Ryvarden, L. and Johansen, I. 1980. A preliminary polypore flora of East Africa. Fungiflora, Oslo, 636pp.


27. Sánchez Márquez, S., Bills G.F., Domínguez Acuña, L. and Zabalgogeazcoa, I. 2010. Endophytic mycobiota of leaves and roots of the grass Holcus lanatus. Fungal Diversity 41: 115-123.


28. Shahiri Tabarestani, M., Rahnam, K., Nasrollanejad. S. and Fatemi. M.H. 2016. Identification of Volatile Organic Compounds from Trichoderma virens (6011) by GC-MS and Separation of a Bioactive Compound via Nanotechnology. International Journal of Engineering, 29 (10): 1347-1353.


29. Shamouna, S.F. and Sieber, T.N. 2000. Colonisation of leaves and twigs of Rubus parviflorus and R. spectabilis by endophytic fungi in a reforestation site in British Columbia. Mycological Research, 104: 841-845.


30. Shimada, K., Fujikawa, K., Yahara, K., and Nakamura, T. 1992. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricuture and Food Chemistry, 40 (6): 945-948.


31. Silva, F., Ferreres, J.O. and Malva, A.C.P. 2005. Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chemistry, 90 (2): 157-167.


32. Srinivasan, K., Jagadish, L.K., Shenbhagaraman, R. and Muthumary, J.  2010. Antioxidant activity of endophytic fungus phyllosticta sp. isolated from guazuma tomentosa. Journal of Phytology, 2(6): 37-41.


33. Strobel, G., Yang, X., Sears, J., Kramer, R., Sidhu, R. S. and Hess, W. M. 1996. Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallichiana.Microbiology, 142: 435-440.


34. Taga, M.S., Miller, E.E. and Pratt, D.E. 1984. Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists Society, 61: 928-993.


35. Tan, R.X., and Zou, W.X. 2001. Endophytes: a rich source of functional metabolites. Natural Product Reports, 18: 448-459.


36. Vilkha, K., Mawson, R., Simons, L. and Bates, D. 2008. Application and opportunities for untrasound assisted extraction in food industry; a review. Innovative Food Science and Emerging Technologies, 9: 161-169.