The study of salty stresses (NaCl & CaCl2) and ascorbic acid on physiological charecterestics of Carpobrotus edulis L.

Subject Areas : Genetic

Rezaei  M.A ¹ (Department of biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran.)
S.Z  Hosseini, ² (Member of Yong Researchers Club, Islamic Azad University, Gorgan Branch, Iran.)
مریم  Safaei, ³ (Member of Yong Researchers Club, Islamic Azad University, Gorgan Branch, Iran.)
فریبا  Amirlatifi ⁴ (Islamic Azad University, Gorgan Branch, Iran.)

Received: 2008-04-27 Accepted : 2008-09-15 Published : 2009-03-15

Keywords: Salinity, total protein, Calcium, Proline, peroxidase, Ascorbate, glycine betaine, soluble sugar, Ice Plant, Raphide,

Abstract :

In order to study of effects of salinity stress on Ice plant (Carpobrotus edulis L.), three kind of soil with 1, 8 and 12ds/m selected for poted experiments. Before NaCl and CaCl2 treatments and after vegetative generation, ascorbate was treated in two concentrations of 2.5 and 5 mmol.  CaCl2 treatments supplemented as 25 and 50 percent of salt treatment. Results indicated that with increasing of salinity (EC), calcium and ascorbate treatments, Na+, Cl-, Ca+2 and K+ of leaves increased significantly, in comparison with control. CaCl2 and ascorbate treatments have no diminishing effects on salinity stress intensity and Na+ absorption. Plant did not use soluble sugar and proline as an osmolyte for increasing osmotic pressure. Glycine betaine and total protein contents increased with increasing of salinity stress and plant got included among plants with the strategy of production and accumulation of glycine betaine and did not use proline. Suitable need of calcium for amelioration of peroxidase activity for scavenging of reactive oxygen species was different in salinities. Calcium oxalate and raphide morphology and raphide bundles number in cross section of leaves and raphides needles number in volume unit had no specific physiological trend and did not change significantly and was not affected by soil salinity, calcium concentration and ascorbate treatments.

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