The effect of screening and optimization of osmopriming on seed germination properties of wheat (Triticum aestivum L.) using surface-response method
Subject Areas : Genetic
Afagh
Yavari
1
(department of biology, Pnu university, Tehran, Iran.)
Ghader
Habibi
2
(Department of Biology, Payame Noor University, Tehran, Iran)
Masumeh
Abedini
3
(Department of Biology, Payame Noor University , Tehran, Iran)
Gholamreza
Bakhshi Khaniki
4
(Department of Biology, Payame Noor University, 19395-3697 Tehran, Iran.)
Keywords: Modeling, Seed yield, Experimental design, Osmotic compounds, Box- Benken design,
Abstract :
Seed osmopriming is an effective strategy to improve the germination process. Screening and estimating the optimal concentrations of different osmotic compounds is the first step in seed osmopriming. In this study, the effects of treatment with single osmotic compounds were initially evaluated at different concentrations, namely NaCl (0, 1, 2, 3, 4, 5, 10, 20, 30 g/l), ZnSO4 (0, 1, 5, 10, 20, 30 and 40 mM), proline (0, 1, 5, 10, 20, 30 and 40 mM), and trehalose (0, 1, 5, 10, 20, 30 and 40 mM) on wheat seeds. Then, the surface-response method was designed in the form of a Box-Benken design. Results showed a significant increase in wheat seed germination at concentrations of 3 and 10 g/l NaCl, 1 and 20 mM ZnSO4, 1 and 10 mM proline, and 0 and 1 mM trehalose. These concentrations were validated using the surface-response method and experiments. The values of coefficient of determination (R2 = 0.99) and adjusted R2 (R2adj = 0.99) showed that the obtained model is suitable for data analysis. One-way analysis of variance confirmed the quadratic model as the best model to determine the interaction of the studied variables (P<0.0001). The optimal values were proposed as a combination of 3 g/l NaCl, 1 mM ZnSO4, and 1 mM proline. In another experiment, using the values predicted by the model, it was found that the surface-response method can be used in screening and germination optimization tests. The results of the follow up experiment using the values determined in the model revealed that the surface-response method can be considered in screening and optimization studies of seed germination.
Abbasi, A., Shekari, F., Mousavi, S.B., Sabaghnia, N. and Javanmard, A. (2017). The partitioning trend of resources and Alpha-Amylase enzyme activity with Zinc priming in wheat (Triticum aestivum) seed. Iranian Journal of Seed Research. 3(2): 1-13.
Abid, M., Hakeem, A., Shao, Y., Liu, Y., Zahoor, R., Fan, Y., Suyu, J., Ata-Ul-Karim, S.T., Tian, Z. and Jiang, D. (2018). Seed osmopriming invokes stress memory against post-germinative drought stress in wheat (Triticum aestivum L.). Environmental andExperimental Botany. 145: 12-20.
Abnavi, M.S. and Ghobadi, M. (2012). The effects of source of priming and post-priming storage duration on seed germination and seedling growth characteristics in wheat (Triticum aestivem L.). Journal of Agricultural Science. 4(9): 256.
Alizadeh, M. and yadavi, A. (2016). The effect of priming and irrigation water quality on seed and oil yield and yield components of two sesames (Sesamum indicum L.). Journal of Plant Productions (Agronomy, breeding and horticulture). 39(2): 115-125.
Ashraf, C. and Abu-Shakra, S. (1978). Wheat seed germination under low temperature and moisture stress. Agronomy Journal. 70(1): 135-139.
Bose, B. and Singhal, R. (2020). Drought stress responses of wheat varieties (Triticum aestivum L.) using Mg (NO3)2 and ZnSO4 primed seeds.
Dell'Aquila, A. and Taranto, G. (1986). Cell division and DNA-synthesis during osmopriming treatment and following germination in aged wheat embryos. Seed Science and Technology. 14(2): 333-341.
Dell’Aquila, Pignone, A.D. and Carella, G. (1984). Polyethylene glycol 6000 priming effect on germination of aged wheat seed lots. Biologia Plantarum. 26(3): 166.
Ebrahimi, N. and Kaboli, S.H. (2020). Effect of different times and KNO3 concentrations on Silybum marianum seedling enhancement. Journal of Medicinal Plants and By-product. 9(1): 51-58.
Farooq, M., Irfan, M., Aziz, T., Ahmad, I. and Cheema, S. (2013). Seed priming with Ascorbic acid improves drought resistance of wheat. Journal of Agronomy and Crop Science. 199(1): 12-22.
Farooq, S., Hussain, M., Jabran, K., Hassan, W., Rizwan, M.S. and Yasir, T.A. (2017). Osmopriming with CaCl2 improves wheat (Triticum aestivum L.) production under water-limited environments. Environmental Science and PollutionResearch. 24(15): 13638-13649.
Feghhenabi, F., Hadi, H., Khodaverdiloo, H. and Van- Genuchten, M.T. (2020). Seed priming alleviated salinity stress during germination and emergence of wheat (Triticum aestivum L.). Agricultural Water Management. 231: 106022.
Finch-Savage, W.E. and Leubner-Metzger, G. (2006). Seed dormancy and the control of germination. New phytologist. 171(3): 501-523.
Fuller, M.P., Hamza, J.H., Rihan, H.Z. and Al-Issawi, M. (2012). Germination of primed seed under NaCl stress in wheat. International Scholarly Research Notices 2012.
Khafagy, M., Mohamed, A.H., Farouk, S. and Amrajaa, H.K. (2017). Effect of pre-treatment of barley grain on germination and seedling growth under drought stress. Advances in Applied Sciences. 2(3): 33-42.
Khan, A., Shafi, M., Bakht, J. and Anwar, S. (2017). Effect of salinity and seed priming on growth characters of wheat varieties. Sarhad Journal of Agriculrure. 33: 435-446.
Lemmens, E., Deleu, L.J., De Brier, N., De Man, W.L., De Proft, M., Prinsen, E. and Delcour, J.A. (2019). The impact of hydro-priming and osmo-priming on seedling characteristics, plant hormone concentrations, activity of selected hydrolytic enzymes, and cell wall and phytate hydrolysis in sprouted wheat (Triticum aestivum L.). ACS Omega. 4(26): 22089-22100.
Lutts, S., Benincasa, P., Wojtyla, L., Kubala, S., Pace, R., Lechowska, K., Quinet, M. and Garnczarska, M. (2016). Seed priming: new comprehensive approaches for an old empirical technique. New Challenges in Seed Biology-Basic and Translational Research Driving Seed Technology: 1-46.
Mahawar, M.K., Samuel, D., Sinha, J. and Jalgaonkar, K. (2018). Optimization of Hydropriming of Okra (Abelmoschus esculentus) Seeds Using Response Surface Methodology. Agricultural Research. 7(1): 25-36.
Mahawar, M.K., Samuel, D., Sinha, J. and Jalgaonkar, K. (2016). Optimization of pea (Pisum sativum) seeds hydropriming by application of response surface methodology. Acta Physiologiae Plantarum. 38(9): 1-13.
Manmathan, H., Shaner, D., Snelling, J., Tisserat, N. and Lapitan, N. (2013). Virus-induced gene silencing of Arabidopsis thaliana gene homologues in wheat identifies genes conferring improved drought tolerance. Journal of Experimental Botany 64(5): 1381-1392.
Mirmazloum, I., Kiss, A., Erdélyi, É., Ladányi, M., Németh, É.Z. and Radácsi, P. (2020). The effect of osmopriming on seed germination and early seedling characteristics of Carum carvi L. Agriculture. 10(4): 94.
Misra, N. and Dwivedi, D. (1980). Effects of pre-sowing seed treatments on growth and dry-matter accumulation of high-yielding wheat under rainfed conditions. Indian Journal of Agronomy.
Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G.A., Sajid, M., Subtain, M.U. and Shabbir, I. (2013). Seed priming a technique. International Jjournal of Agriculture And Crop Sciences. 6(20): 1373.
Paul, S. and Choudhury, A. (1991). Effect of seed priming with potassium salts on growth and yield of wheat under rainfed condition.
Peng, X., Yang, G., Shi, Y., Zhou, Y., Zhang, M. and Li, S. (2020). Box–Behnken design based statistical modeling for the extractgion and physicochemical properties of pectin from sunflower heads and the comparison with commercial low-methoxyl pectin. Scientific Reports. 10(1): 1-10.
Pereira, E.G., Amaral, M.B., Bucher, C.A., Santos, L.A., Fernandes, M.S. and Vieira Rossetto, C.A. (2021). Proline osmopriming improves the root architecture, nitrogen content and growth of rice seedlings. Biocatalysis and Agricultural Biotechnology. 33: 101998.
Perveen, S. and Nazir, M. (2018). Proline treatment induces salt stress tolerance in maize (Zea Mays L. cv. Safaid afgoi). PakistanJournal of Botany. 50(4): 1265-1271.
Rezai, A., Balouchi, H., Movahhedi Dehnavi, M. and Adhami, I. (2018). Effect of different priming on seed germination indices and enzyme of sorghum (Sorghum bicolor L.) SOR834 genotype under cadmium chloride and nitrate toxicity. Journal of Plant Productions (Agronomy, Breeding and Horticulture). 41(1): 69-82.
Safari, k., sohrabi, y., Siosemardeh, A. and sasani, S. (2019). Effect of seed priming on some shoot and root morphophysiological characteristics of bread wheat (Triticum aestivum L.) in potted planting conditions in farm. Journal of Plant Productions (Agronomy, Breeding and hHorticulture).
Saleh, J. and Maftoun, M. (2008). Interactive effects of NaCl levels and zinc sources and levels on the growth and mineral composition of rice. Journal of AgriculturalScience and Technology. 10(4): 325-336.
Sharma, M. and Parmar, D. (2018). Effect of seed priming with zinc sulfate on yield and quality parameters of rainfed maize-pea sequence under mid hill conditions of Himachal Pradesh. Journal of Pharmacognosy and Phytochemistry. 7(1): 1401-1407.
Singh, A., Dahiru, R., Musa, M. and Sani Haliru, B. (2014). Effect of osmopriming duration on germination, emergence, and early growth of Cowpea (Vigna unguiculata (L.) Walp.) in the Sudan Savanna of Nigeria. International Journal of Agronomy 2014.
Soughir, M., Aymen, E.M. and Cherif, H. (2012). Effect of NaCl priming duration and concentration on germination behavior of fenugreek. Albanian Journal of Agricultural Sciences. 11(4): 193.
Stephen, K., Khan, F., Bhat, S., Narayan, S., Mir, S., Mir, M., Hussain, K., Gul, M., Khurshid, A. and Siddiqi, I. (2018). Optimizing priming concentration and duration of various priming agents for improved seed germination in chilli (Capsicum annum L.). Journal of Pharmacognosy and Phytochemistry. 7(4): 2689-2693.
Vahdati, M., Aghdasi, M. and Sadeghipour, H.R. (2010). Interaction of trehalose and ascorbic acid in growing Arabidopsis seedlings. Journal of Plant Production (Journal of Agricultural Sciences and Natural Resources). 17(4): 27-48.
Varier, A., Vari, A.K. and Dadlani, M. (2010). The subcellular basis of seed priming. Current Science. 99(4): 450-456.
Yari, L., Aghaalikani, M. and Khazaei, F. (2010). Effect of seed priming duration and temperature on seed germination behavior of bread wheat (Triticum aestivum L.). ARPN Journal of Agricultural and Biological Science. 5(1): 1-6.
Yohannes, G. and Abraha, B. (2013). The role of seed priming in improving seed germination and seedling growth of maize (Zea mays L.) under salt stress at laboratory conditions. African Journal of Biotechnology. 12(46): 6484-6490.
Zare, N., Sadat Noori, S., Kholgh Sima, N.A.K. and Mortazavian, S.M.M. (2014). Effect of laser priming on accumulation of free proline in spring durum wheat (Triticum turgidum L.) under salinity stress. International Transaction Journal of Engineering, Management, and Applied Sciences and Technologies. 5(2): 119-130.
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Abbasi, A., Shekari, F., Mousavi, S.B., Sabaghnia, N. and Javanmard, A. (2017). The partitioning trend of resources and Alpha-Amylase enzyme activity with Zinc priming in wheat (Triticum aestivum) seed. Iranian Journal of Seed Research. 3(2): 1-13.
Abid, M., Hakeem, A., Shao, Y., Liu, Y., Zahoor, R., Fan, Y., Suyu, J., Ata-Ul-Karim, S.T., Tian, Z. and Jiang, D. (2018). Seed osmopriming invokes stress memory against post-germinative drought stress in wheat (Triticum aestivum L.). Environmental andExperimental Botany. 145: 12-20.
Abnavi, M.S. and Ghobadi, M. (2012). The effects of source of priming and post-priming storage duration on seed germination and seedling growth characteristics in wheat (Triticum aestivem L.). Journal of Agricultural Science. 4(9): 256.
Alizadeh, M. and yadavi, A. (2016). The effect of priming and irrigation water quality on seed and oil yield and yield components of two sesames (Sesamum indicum L.). Journal of Plant Productions (Agronomy, breeding and horticulture). 39(2): 115-125.
Ashraf, C. and Abu-Shakra, S. (1978). Wheat seed germination under low temperature and moisture stress. Agronomy Journal. 70(1): 135-139.
Bose, B. and Singhal, R. (2020). Drought stress responses of wheat varieties (Triticum aestivum L.) using Mg (NO3)2 and ZnSO4 primed seeds.
Dell'Aquila, A. and Taranto, G. (1986). Cell division and DNA-synthesis during osmopriming treatment and following germination in aged wheat embryos. Seed Science and Technology. 14(2): 333-341.
Dell’Aquila, Pignone, A.D. and Carella, G. (1984). Polyethylene glycol 6000 priming effect on germination of aged wheat seed lots. Biologia Plantarum. 26(3): 166.
Ebrahimi, N. and Kaboli, S.H. (2020). Effect of different times and KNO3 concentrations on Silybum marianum seedling enhancement. Journal of Medicinal Plants and By-product. 9(1): 51-58.
Farooq, M., Irfan, M., Aziz, T., Ahmad, I. and Cheema, S. (2013). Seed priming with Ascorbic acid improves drought resistance of wheat. Journal of Agronomy and Crop Science. 199(1): 12-22.
Farooq, S., Hussain, M., Jabran, K., Hassan, W., Rizwan, M.S. and Yasir, T.A. (2017). Osmopriming with CaCl2 improves wheat (Triticum aestivum L.) production under water-limited environments. Environmental Science and PollutionResearch. 24(15): 13638-13649.
Feghhenabi, F., Hadi, H., Khodaverdiloo, H. and Van- Genuchten, M.T. (2020). Seed priming alleviated salinity stress during germination and emergence of wheat (Triticum aestivum L.). Agricultural Water Management. 231: 106022.
Finch-Savage, W.E. and Leubner-Metzger, G. (2006). Seed dormancy and the control of germination. New phytologist. 171(3): 501-523.
Fuller, M.P., Hamza, J.H., Rihan, H.Z. and Al-Issawi, M. (2012). Germination of primed seed under NaCl stress in wheat. International Scholarly Research Notices 2012.
Khafagy, M., Mohamed, A.H., Farouk, S. and Amrajaa, H.K. (2017). Effect of pre-treatment of barley grain on germination and seedling growth under drought stress. Advances in Applied Sciences. 2(3): 33-42.
Khan, A., Shafi, M., Bakht, J. and Anwar, S. (2017). Effect of salinity and seed priming on growth characters of wheat varieties. Sarhad Journal of Agriculrure. 33: 435-446.
Lemmens, E., Deleu, L.J., De Brier, N., De Man, W.L., De Proft, M., Prinsen, E. and Delcour, J.A. (2019). The impact of hydro-priming and osmo-priming on seedling characteristics, plant hormone concentrations, activity of selected hydrolytic enzymes, and cell wall and phytate hydrolysis in sprouted wheat (Triticum aestivum L.). ACS Omega. 4(26): 22089-22100.
Lutts, S., Benincasa, P., Wojtyla, L., Kubala, S., Pace, R., Lechowska, K., Quinet, M. and Garnczarska, M. (2016). Seed priming: new comprehensive approaches for an old empirical technique. New Challenges in Seed Biology-Basic and Translational Research Driving Seed Technology: 1-46.
Mahawar, M.K., Samuel, D., Sinha, J. and Jalgaonkar, K. (2018). Optimization of Hydropriming of Okra (Abelmoschus esculentus) Seeds Using Response Surface Methodology. Agricultural Research. 7(1): 25-36.
Mahawar, M.K., Samuel, D., Sinha, J. and Jalgaonkar, K. (2016). Optimization of pea (Pisum sativum) seeds hydropriming by application of response surface methodology. Acta Physiologiae Plantarum. 38(9): 1-13.
Manmathan, H., Shaner, D., Snelling, J., Tisserat, N. and Lapitan, N. (2013). Virus-induced gene silencing of Arabidopsis thaliana gene homologues in wheat identifies genes conferring improved drought tolerance. Journal of Experimental Botany 64(5): 1381-1392.
Mirmazloum, I., Kiss, A., Erdélyi, É., Ladányi, M., Németh, É.Z. and Radácsi, P. (2020). The effect of osmopriming on seed germination and early seedling characteristics of Carum carvi L. Agriculture. 10(4): 94.
Misra, N. and Dwivedi, D. (1980). Effects of pre-sowing seed treatments on growth and dry-matter accumulation of high-yielding wheat under rainfed conditions. Indian Journal of Agronomy.
Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G.A., Sajid, M., Subtain, M.U. and Shabbir, I. (2013). Seed priming a technique. International Jjournal of Agriculture And Crop Sciences. 6(20): 1373.
Paul, S. and Choudhury, A. (1991). Effect of seed priming with potassium salts on growth and yield of wheat under rainfed condition.
Peng, X., Yang, G., Shi, Y., Zhou, Y., Zhang, M. and Li, S. (2020). Box–Behnken design based statistical modeling for the extractgion and physicochemical properties of pectin from sunflower heads and the comparison with commercial low-methoxyl pectin. Scientific Reports. 10(1): 1-10.
Pereira, E.G., Amaral, M.B., Bucher, C.A., Santos, L.A., Fernandes, M.S. and Vieira Rossetto, C.A. (2021). Proline osmopriming improves the root architecture, nitrogen content and growth of rice seedlings. Biocatalysis and Agricultural Biotechnology. 33: 101998.
Perveen, S. and Nazir, M. (2018). Proline treatment induces salt stress tolerance in maize (Zea Mays L. cv. Safaid afgoi). PakistanJournal of Botany. 50(4): 1265-1271.
Rezai, A., Balouchi, H., Movahhedi Dehnavi, M. and Adhami, I. (2018). Effect of different priming on seed germination indices and enzyme of sorghum (Sorghum bicolor L.) SOR834 genotype under cadmium chloride and nitrate toxicity. Journal of Plant Productions (Agronomy, Breeding and Horticulture). 41(1): 69-82.
Safari, k., sohrabi, y., Siosemardeh, A. and sasani, S. (2019). Effect of seed priming on some shoot and root morphophysiological characteristics of bread wheat (Triticum aestivum L.) in potted planting conditions in farm. Journal of Plant Productions (Agronomy, Breeding and hHorticulture).
Saleh, J. and Maftoun, M. (2008). Interactive effects of NaCl levels and zinc sources and levels on the growth and mineral composition of rice. Journal of AgriculturalScience and Technology. 10(4): 325-336.
Sharma, M. and Parmar, D. (2018). Effect of seed priming with zinc sulfate on yield and quality parameters of rainfed maize-pea sequence under mid hill conditions of Himachal Pradesh. Journal of Pharmacognosy and Phytochemistry. 7(1): 1401-1407.
Singh, A., Dahiru, R., Musa, M. and Sani Haliru, B. (2014). Effect of osmopriming duration on germination, emergence, and early growth of Cowpea (Vigna unguiculata (L.) Walp.) in the Sudan Savanna of Nigeria. International Journal of Agronomy 2014.
Soughir, M., Aymen, E.M. and Cherif, H. (2012). Effect of NaCl priming duration and concentration on germination behavior of fenugreek. Albanian Journal of Agricultural Sciences. 11(4): 193.
Stephen, K., Khan, F., Bhat, S., Narayan, S., Mir, S., Mir, M., Hussain, K., Gul, M., Khurshid, A. and Siddiqi, I. (2018). Optimizing priming concentration and duration of various priming agents for improved seed germination in chilli (Capsicum annum L.). Journal of Pharmacognosy and Phytochemistry. 7(4): 2689-2693.
Vahdati, M., Aghdasi, M. and Sadeghipour, H.R. (2010). Interaction of trehalose and ascorbic acid in growing Arabidopsis seedlings. Journal of Plant Production (Journal of Agricultural Sciences and Natural Resources). 17(4): 27-48.
Varier, A., Vari, A.K. and Dadlani, M. (2010). The subcellular basis of seed priming. Current Science. 99(4): 450-456.
Yari, L., Aghaalikani, M. and Khazaei, F. (2010). Effect of seed priming duration and temperature on seed germination behavior of bread wheat (Triticum aestivum L.). ARPN Journal of Agricultural and Biological Science. 5(1): 1-6.
Yohannes, G. and Abraha, B. (2013). The role of seed priming in improving seed germination and seedling growth of maize (Zea mays L.) under salt stress at laboratory conditions. African Journal of Biotechnology. 12(46): 6484-6490.
Zare, N., Sadat Noori, S., Kholgh Sima, N.A.K. and Mortazavian, S.M.M. (2014). Effect of laser priming on accumulation of free proline in spring durum wheat (Triticum turgidum L.) under salinity stress. International Transaction Journal of Engineering, Management, and Applied Sciences and Technologies. 5(2): 119-130.
