Evaluating the foliar application of salicylic acid and iron nano chelate on some biochemical and morpho-physiological properties of Aromatic violet (Viola odorata L.)
Subject Areas : GeneticAsma Hasanvand 1 , Leila Fahmideh 2 , Fatemeh Bidarnamani 3
1 - Department of Medicinal Plants, Spice and Drink, University of Zabol, Zabol, Iran
2 - Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 - Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran
Keywords: Photosynthetic pigments, Carbohydrate, Mucilage, Elicitors, Viola odorata,
Abstract :
To evaluate the effects of different concentrations of salicylic acid and nano iron chelate on the mucilage and carbohydrate values as well as some morpho-physiological traits of Viola aodorata L. species, a factorial experiment was defined based on randomized complete block design with three replications. After planting the seeds in pots, experimental treatments included foliar application of salicylic acid at three levels (0, 0.75 and 1.5 mg / L) as well as nano iron chelate at two levels (0 and 1 g / L) was applied at seedling stage. According to the results, the highest amounts of carotenoid, chlorophyll a, chlorophyll b as well as the total chlorophyll, protein and catalase were observed in specimen treated with 1.5 mg / L salicylic acid. Moreover, the highest amounts of sodium, copper, mucilage and fresh plant weight were detected in specimen treated with 1g / L iron nano chelate. The results also demonstrated that the highest amount of carbohydrate was found in specimen treated with 1.5 mg / L salicylic acid and 1 g / L nano-iron chelate. In this investigation, the foliar application of salicylic acid (1.5 mg / L) and iron nano chelate (1 g / L) led to a 12 and 19 percent increase in the plant height compared to the untreated sample, respectively. Therefore, treatment with salicylic acid and nano-iron chelate could be recommended to increase the growth and medicinal properties of this plant species.
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Agarawal, S., Sairam, R.K., Srivasta, G.C. and Meena, R. C. (2005). Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biological Plant. 49(4): 541-550.
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Arvin, M.J., Beidshki, A., Kramt, B. and Maghsodi, K. (2011). The study salicylic acid (SA) role in contrast with drought stress by affecting on morphological and physiological parameters in garlic plant. In: Proceeding of 7th Iranian Horticultural Science Congress, Isfahan Industrial University, 4-7 September.
Asadi Karam, E., Asrar, Z. and Keramat, B. (2016). Effect of methyl jasmonate on proline content and absorption Cu, Fe, Zn and Mg in Lepidium sativum L. subjected to copper toxicity. Journal of Plant Biology.29(2): 243-253.
Askari, M., Amirjani, M. R. and Saberi, T. (2014). Evaluation of the effects of iron nanofertilizer on leaf growth, antioxidants and carbohydrate contents of Catharanthus roseus. Journal of Plant Process and Function Iranin Society of Plant Physiology. 3 (7): 43-56.
Azad, H., Fakheri, B., Mahdinezhad, N. and Parmoon, Q. (2017). The effect of foliar application of nano-iron chelated on antioxidant enzymes activity and flower yield of chamomile genotypes under drought stress condition. Journal of Crop Improvement. 19(2): 257-271.
Azadbakht, M. (1999). Classification of herbs, Vol.1, Teymoorzade’s Publication, 420 pp.
Baghaie, N. and Maleki Farahani, S. (2014). Comparison of nano and micro chelated iron fertilizers on quantitative yield and assimilates allocation of saffron (Crocus sativus L.). Journal of Saffron Research. 1(2): 156-169.
Bahrani, A.M. and Pourreza, A. (2014). Effects of micronutrients on seed yield and oil content of Brassica napus L. CV Talaeh. Botany. 43(2): 231-233.
Barghi, A., Gholipoori, A., Tobeh, A., Jahan Bakhsh, S., and Jamaati, S. (2014). Survey on the effects of iron nano oxide foliar application on mineral nutrients uptake in potato tuber. Journal of Plant Ecophysiology, 6(16), 1-12.
Bayat, H., Alirezaie, M. and Neamati, H. (2012). Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L.) under salinity stress. Journal Stress Physiology Biochemistry. 8: 258-267.
Beers, G.R. and Sizer, I. V. (1952). A spectrophotometric method for measuring the break down of hydrogen peroxide by catalase. Journal of Biological Chemistry. 195: 133-140.
Bradford, M. M. (1976). A rapiand sensitive method for quantitation of microgram quantitiesof protein utilizing the principle of protein-dye binding. Journal of Analytical Biochemistry. 72: 248- 254.
Dahmardeh, M., Taji, R., Khammari, I. and Hadad, T. (2018). Evaluation of the green synthesis iron and silver nanoparticles of green tea (Camellia sinesis L.) extract on morphological and physiological traits of borage (Borago officinalis L.). Iranian Journal of Medicinal and Aromatic Plants. 34(2): 330-345.
Davoudi, S., Biabani, A., rahemi-karizaki, A., modares sanavi, S., Gholamalipor Alamdari, E., and Zarei, M. (2020). Effects of nano and common forms of iron and zinc chelates on some characteristics of medicinal plant black cumin (Nigella sativa L.). Iranian Journal of Medicinal and Aromatic Plants Research, 36(3), 448-465.
El-Tayeb, M. A. (2005). Response of barley grain to the interactive effect of salinity and salicylic
acid. Plant Growth Regulation. 42: 215-224.
Enteshar, Sh. and Esnaashari, E. (2018). Effects of iron chloride, iron chelate and nano-iron on enzymatic and non-enzymatic antioxidant mechanisms in Melissa officinalis under aluminum treatment. Journal of Plant Process and Function. 7(23): 193-204.
Eraslan, F., Inal, A., Pilbeam, DJ. and Gunes, A. (2008). Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinacia oleracea L. CV. Matador) grown under boron toxicity and salinity. Plant Growth Regulation. 55: 207-219.
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