ارزیابی اثرات محلول پاشی اسید سالیسیلیک و نانو کلات آهن بر برخی خصوصیات بیوشیمیایی و مورفوفیزیولوژیکی بنفشه معطر (odorata L. Viola)
محورهای موضوعی : ژنتیکاسماء حسنوند 1 , لیلا فهمیده 2 , فاطمه بیدرنامنی 3
1 - گروه گیاهان دارویی، ادویه‌ای و نوشابه‌ای دانشگاه زابل، زابل، ایران
2 - گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
3 - گروه زراعت و اصلاح نباتات، پژوهشکده کشاورزی، پژوهشگاه زابل، زابل، ایران
کلید واژه: کربوهیدرات, موسیلاژ, رنگیزه‌های فتوسنتزی, القا کننده, بنفشه معطر,
چکیده مقاله :
به منظور ارزیابی تأثیر غلظت‌های مختلف اسید سالیسیلیک و نانو کلات آهن بر میزان موسیلاژ، کربوهیدرات ها و برخی صفات مورفوفیزیولوژیکی گیاه بنفشه معطر، آزمایشی به صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار انجام شد. پس از کشت بذرها در گلدان، تیمارهای آزمایشی شامل محلول پاشی اسید سالسیلیک (در سه سطح صفر (شاهد)، 75/0 و 5/1 میلی‌گرم در لیتر) و نانو کلات آهن (در دو سطح صفر (شاهد) و 1 گرم در لیتر) در مرحله گیاهچه ای اعمال شد. نتایج حاصله نشان داد که بیشترین مقادیر کاروتنوئیدها، کلروفیل a، b و کل، پروتئین و فعالیت کاتالاز در گیاهان محلول پاشی شده با 5/1 میلی‌گرم در لیتر اسید سالسیلیک حاصل شد و همچنین بیشترین مقدار عناصر معدنی سدیم، مس، موسیلاژ و وزن‌تر بوته هم در تیمار محلول پاشی یک گرم در لیتر نانو کلات وجود داشت. سایر نتایج نیز مشخص ساخت که بیشترین مقدار کربوهیدرات در محلول پاشی تیمار 5/1 میلی‌گرم در لیتر اسید سالسیلیک و یک گرم در لیتر نانو کلات آهن بدست آمد. در این مطالعه افزایش 12 و 19 درصدی ارتفاع در بوته های محلول پاشی با اسید سالسیلیک (5/1 میلی‌گرم در لیتر) و نانو کلات آهن (1 گرم در لیتر) نسبت به شاهد مشاهده شد. بر اساس نتایج حاصله می توان تیمار اسید سالسیلیک و نانو کلات آهن را به منظور افزایش خصوصیات رشدی و داروئی این گیاه پیشنهاد نمود.
To evaluate the effects of different concentrations of salicylic acid and nano iron chelate on the mucilage and carbohydrate values as well as some morpho-physiological traits of Viola aodorata L. species, a factorial experiment was defined based on randomized complete block design with three replications. After planting the seeds in pots, experimental treatments included foliar application of salicylic acid at three levels (0, 0.75 and 1.5 mg / L) as well as nano iron chelate at two levels (0 and 1 g / L) was applied at seedling stage. According to the results, the highest amounts of carotenoid, chlorophyll a, chlorophyll b as well as the total chlorophyll, protein and catalase were observed in specimen treated with 1.5 mg / L salicylic acid. Moreover, the highest amounts of sodium, copper, mucilage and fresh plant weight were detected in specimen treated with 1g / L iron nano chelate. The results also demonstrated that the highest amount of carbohydrate was found in specimen treated with 1.5 mg / L salicylic acid and 1 g / L nano-iron chelate. In this investigation, the foliar application of salicylic acid (1.5 mg / L) and iron nano chelate (1 g / L) led to a 12 and 19 percent increase in the plant height compared to the untreated sample, respectively. Therefore, treatment with salicylic acid and nano-iron chelate could be recommended to increase the growth and medicinal properties of this plant species.
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