Phytochemical analysis of various organs of Rheum ribes weed at the phonological stage of flowering (Case study: heights of Karizak Village of Kashmar)
Subject Areas : Geneticابراهیم Gholamalipour Alamdari 1 , فاطمه Makari 2 , جواد Bayat Kohsar 3
1 - گروه تولیدات گیاهی، دانشکده کشاورزی و منابعطبیعی دانشگاه گنبدکاووس، ایران
2 - گروه علوم دامی، دانشکده کشاورزی و منابعطبیعی دانشگاه گنبدکاووس، ایران
3 - گروه علوم دامی، دانشکده کشاورزی و منابعطبیعی دانشگاه گنبدکاووس، ایران
Keywords: Rheum ribes, Organs, Chemical analysis, crud protein, soluble carbohydrates, Total phenols, phonological stage of flowering,
Abstract :
An experiment was conducted for phytochemical analysis of the compounds of Rheum ribes weedat the phonological stage of flowering in Weed Science Laboratory of Gonbad-e Qabus University in 2015. Plant samples of Rheum ribes were collected from heights of Karizak Village of Kashmar. After identifying the Rheum species, various organs of stem, leaf, and inflorescence were separated from each other, dried and finally powdered. Chemical compounds like the organic matter, ash, crud protein, ADF, NDF, starch, soluble carbohydrates, and total phenols contents of the organs under study and a combination of organs were measured using the standardized phytochemical methods. Results of the variance analysis showed that there were significant differences in the phytochemical traits among various organs of Rheum ribes. Findings suggest that the highest percentage of organic matter, ash, crud protein, NDF, and ADF were obtained in the inflorescence, both the stem and leaf, leaf and inflorescence, and stem and inflorescence, respectively. The study also recorded the highest levels of total phenols in both in floresvence and combination of the organs while the lowest phenols contents were observed in the stem. Results generally showed variations in the content of phytochemical compounds under study in Rheum ribes organs. Therefore, regarding high productivity of Rheum ribes biomass and the role of phenolic compounds as well as suitable content of some primary metabolites, optimization of suitable method to extract these compounds is necessary.
Alaoui, B., Genet, P., Dunand, F.V., Toussaint, M.L., Epron, D. and Bardot, P. M. (2003). Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationship with carbohydrate accumulation and change in ion contents. Plant Science. 166: 1213-1218.
AOAC. (2003). Official Methods of Analysis of AOAC International. 17th edition. 2nd revision. Gaithersburg, MD, USA, Association of Analytical Communities.
AOAC. (2005). Official Methods of Analysis. Vol. 1. No. 1. 18th ed. Association of Official Analytical Chemists Washing Town, D.C.
Dholwani, K.K., Saluja, A.K., Gupta, A.R. and Shah, D. R. (2008). A review on plant-derived natural products and their analogs with anti-tumor activity. Indian Journal of Pharmacology. 40: 49-58.
Emad, M., Ghibi, F., Rasoli, S.M., Khanzadeh, R. and Jozani, S.M. (2011). Medicinal and industrial plant of Rheum ribes. Publication of Poone. P: 69.
Everitt, J.H., Alaniz, M.A. and Gerbermann, A.H. (1982). Chemical composition of native range grasses, growing on saline soils of the South Texas Plains. Journal of Range Management. 35(1): 43-46.
Ghahreman, A. (1988). Iranian flora. Publication of forestry and rangelands of Iran. 4: 187.
Heim, K.E., Tagliaferro, A.R. and Bobilya, D.J. (2002). Flavonoid antioxidants: chemistry, metabolism and structure activity relationships. Journal Nutrient Biochemical. 13(10): 572-584.
Hirt, H. and Shinozaki, K. (2004). Plant responses to abiotic stress topics in current genetics. Spring berlin/New York.P:78.
Jafari, A., Taheri, Gh., Baradaran, B. and Bahrami, A.R. (2012). Rheum khorasanicum (Polygonaceae), a new species from Iran. Annales Botanici Fennici. 49: 255-258.
Jamshidi, M., Ahmadi, H.R., Rezazadeh, Sh., Fathi, F. and Mazanderani, M. (2010). Study on phenolic and antioxidant activity of some selected plant of Mazandaran province. Medicinal Plants. 9(34): 177-183.
Karaman. S., Tutem, E., Bas-Kan, K.S. and Apak, R. (2010). Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC-CUPRAC assay. Food Chemistry. 120: 1201-9.
Khosravi, M. (2000). Medicinal plants. Publication of Mohammad. 4.P:35-40.
Kochert, G. (1978). Carbohydrate determination by the phenol sulfuric acid method, In Helebust, J.A., Craig, J.S. (ed) Handbook physiological methods, Cambridge University. Press Cambridge. 9697.
Malick, C.P. and Singh, M.B. (1980). In plant enzymology and histo enzymologhy, Kalyani Publishers, New Dehli.
Manieval, S., Thierry, D. and Christiane, G. (2001). Relationship between biomass and phenolic in grain Sorghum grown under different conditions. Agronomy Journal. 93: 49-54.
Mohammadi Milasi, R. and Roshandel, P. (2012). The characteristic of antioxidant and total phenol content in Hyssop plant. National Congress on Medicinal Plants of Yasuj. August 2012.
Nabavi, S.F., Ebrahimzadeh, M.A. and Nabavi, S.M. (2010). Antioxidant activity of flower, stem and leaf extracts of Ferula gummosa Boiss. Grasasy Aceites. 61(3): 277- 250.
Naghiloo, S., Movafeghi, A., Delazar, A., Nazmiye, H., Asnaashari, S. and Dadpour, M.R. (2012). Ontogenetic variation of volatiles and antioxidant activity in leaves of Astragalus compactus Lam. (Fabaceae). EXCLI J. 11: 436 - 43.
Ozcan, M.M., Dursun, N. and Arslan, D. (2007). Some Nutritional Properties of Prangos ferulacea (L.) Lindt and Rheum ribes L. Stems Growing Wild in Turkey. International Journal of Food Sciences and Nutrition. 58 (2): 162-7.
Pedrol, N., Gonzalez, L. and Reigosa, M.L. (2006). Allelopathy and abiotic stress, a Physiological Process with Ecological Implications, Netherlands. 171-209.
Ranjbari, A.R. (1995). Determining elements of dominance rangeland plant of four important regions Isfahan. Master’s Thesis, College of Tarbiat Modares. Tehran. 151 p.
Rojhan, M.S. (2001). Hygienic and remedy with medicinal plant and pharmacognosy. Publication of Tanin.
Rostamza, M. (2004). Evaluation of quantity and quality of some forage cereal (sorghum, panicum and corn) in second cultivation after barley and their effect on yield of further plant. Master’s thesis, Tehran University. 92 p.
Serafini, M., Ghiselli, A. and Luzzi, A.F. (1994). Red wine, tea and antioxidants. Lancet. 344-626.
Srivastava, V., Negi, A.S., Kuma, J. K.R., Gupta, M.M. and Khanuja, S.P.S. (2005). Plant-based anticancer molecules: A chemical and biological profile of some important leads. Bioorganic and Medicinal Chemistry. 13: 5892-590.
Thayumanavan, B. and Sadasivam, S. (1984). Physicochemical basis for the preferential uses of certain rice varieties. Qual plant foods Hum Nutri. 34. 253- 259.
Thomas, G.S. (1990). Perennial Garden Plants J.M. Dent and Sons, London.
Tyler Varro, E., Brady Lynn, R. and Janes, E.R. (1988). Pharmacognosy, 9th Ed. Lea and Febiger Pub. Philadelphia In USA.
Van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991). Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74(10): 3583-3597.
Zargari, A. (1997). Medicinal plants. Publication of Tehran University. 4: 6.
