Study of cardiovascular biomarkers, cystatin-C and adenosine deaminase in alloxan-induced diabetes mellitus in the dog

Subject Areas : Veterinary Clinical Pathology

کاوه  Azimzadeh ¹ (Associate Professor, Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran.)

Received: 2016-04-08 Accepted : 2016-06-30 Published : 2016-07-22

Keywords: Diabetes mellitus, Dog, Cardiovascular biomarkers, Cystatin C, adenosine deaminase,

Abstract :

Diabetes mellitus (DM) is a metabolic disease which may lead to considerable damages in most tissues. The purpose of this study was to assess whether the levels of serum Cardiac troponin I (cTnI), Homocysteine (Hcy), Cystatin c (Cys-C) and adenosine deaminase (ADA) activity are altered in alloxan induced diabetes mellitus in the dog. In this study, DM was induced by alloxan in the treatment group and three months later, plasma cTnI, Hcy and Cys-C were evaluated by Elisa technique and ADA activity was assessed by Electrochemilumminescence (ECL) method. The results showed significant increases (p<0.01) in cTnI and Cys-C along with a considerable decrease in ADA in the treatment group in comparison to the intact healthy group. Conclusively, increased plasma levels of cTnI and Cys-C in dogs with DM indicates heart and kidney damage respectively, which requires effective measures in the management of diabetes mellitus in dogs. Furthermore, reduction of ADA activity can be attributed to the decline of immune system (especially T cells), high levels of adenosine concentrations for facilitation of glucose transport and/or zinc ion decrease.

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