Veterinary Clinical Pathology The Quarterly Scientific Journal: 2 (38)

Open Access Article

1 - Effect of monensin, yeast, selenoprotein and sodium selenite supplementation in flushing period on some blood elements and sexuality of newborn lambs in Ghezel ewes
سامان Saedi حسین Daghigh Kia لیلا Ahmadzadeh علی Hosseinkhani

Some dietary ions can facilitate the penetration of certain types of spermatic chromosomes in the layer surrounding the oocyte to change the sex ratio of newborn lambs. To check this, 66 Ghezel ewes were used in six groups (11 in each group): Treatments consisted of gro More

Some dietary ions can facilitate the penetration of certain types of spermatic chromosomes in the layer surrounding the oocyte to change the sex ratio of newborn lambs. To check this, 66 Ghezel ewes were used in six groups (11 in each group): Treatments consisted of group A as a control (basal diet), group B (flushing with barley grain), group C (flushing with barley grain+vitamin E+organic selenium), group D (flushing with barley grain+vitamin E+mineral selenium), group E (flushing with barley grain+monensin) and group F (flushing with barley grain+yeast). Ewes received experimental diets for 5 weeks (two weeks before and three weeks after mating). Magnesium, calcium, sodium and potassium of serum were measured. The results showed that ewes in groups C and D had the lowest level of calcium and magnesium and highest level of sodium and potassium in the time of estrous and 21 days after mating (p Manuscript profile

Open Access Article

2 - Coprologic survey on ruminant's fasciolosis in east Azerbaijan province during 2014-2015
عباس Imani Baran حبیب Cheraghi Saray

Fasciolosis is a zoonotic disease of humans and animals causing health problems and great economic losses in different parts of the world including Iran. Fasciola hepatica and Fasciola gigantica are well-known agents for fasciolosis. Due to health and economic implicati More

Fasciolosis is a zoonotic disease of humans and animals causing health problems and great economic losses in different parts of the world including Iran. Fasciola hepatica and Fasciola gigantica are well-known agents for fasciolosis. Due to health and economic implications of this disease and the lack of field studies on fasciolosis in living ruminants of East Azerbaijan, the current study was carried out to determine fasciolosis status in living ruminants from different regions of East Azerbaijan province based on coprologic method for the first time. For this purpose, a total of 2012 fecal samples (1782 from sheep and 230 from cattle) were collected and examined under light microscope. The microscopic findings showed that of all the samples, 62 (3.48%) from sheep and 10 (4.35%) from cattle were infected with Fasciola spp. eggs. A total of 72 (3.58%) fecal samples were infected by fasciola eggs. Geographically, the highest percentage of infection in cattle and sheep were observed in the northern regions of province with 6.14% and 5.21% respectively. In terms of age the infection rates in cattle under and over 2 years were 3.81% (4.105) and 4/8% (6.125) respectively whereas those for sheep were 4.38% (41.937) and 2.49% (21.845). The results indicated presence of fasciolosis amongst ruminants of the province necessitating further studies to detect the unknown aspects of disease and regular antiparasitic treatments for its prevention. Manuscript profile

Open Access Article

3 - Study of cloisonne kidney lesion frequency in slaughtered sheeps of Tabriz and Rashta slaughterhouse
آرش Khaki

Cloisonne kidney is an accidental finding of the kidneys observed in abattoir. The lesion caused by the thickening of the proximal and sometimes distal convoluted tubules basement membranes due to iron pigmentation is so far reported in goats, sheep and horses. Renal co More

Cloisonne kidney is an accidental finding of the kidneys observed in abattoir. The lesion caused by the thickening of the proximal and sometimes distal convoluted tubules basement membranes due to iron pigmentation is so far reported in goats, sheep and horses. Renal cortex is brown but the condition does not involve the medulla. The lesion brings about no impairment of the renal normal function and affected animals are clinically normal. Although the exact cause of this condition remains unknown but intravascular hemolysis is considered to have a role. The following study was undertaken to determine sheep cloisonne kidney frequency rate in Tabriz and Rasht abattoir. A number of 10000 sheep carcasses in Rasht slaughterhouse and 8600 carcasses in Tabriz slaughterhouse were randomly examined and tissue samples were stained with H&E. Only eleven cases were diagnosed with the cloisonne kidney lesion. Thus the frequency rate of the lesion was estimated at 0.059% in sheep of these cities. Manuscript profile

Open Access Article

4 - Isolation, culture expansion and characterization of canine bone marrow derived mesenchymal stem cells
داود Kazemi کریم Shams Asenjan نیما Dehdilani حامد Parsa A.A Movassagh Pour Akbari پروین Akbarzadeh

The purpose of the present study was to isolate, culture expand and characterize canine bone marrow derived mesenchymal stem cells. Bone marrow aspirates of 15 adult male dogs were collected to this end and their mononuclear cells isolated by centrifugation and cultured More

The purpose of the present study was to isolate, culture expand and characterize canine bone marrow derived mesenchymal stem cells. Bone marrow aspirates of 15 adult male dogs were collected to this end and their mononuclear cells isolated by centrifugation and cultured in standard media. The adherent cells were isolated and their mesenchymal origin was confirmed at 3rd passage by cellular morphology, expression of surface antigens and differentiation to osteogenic and adipogenic lineage. After 4 days, spindle shaped fibroblast like cells which were apparently bone marrow derived mesenchymal stem cells appeared in culture medium and their numbers increased over time. The cells reached 3rd passage with over 75% confluent after a mean of 22.89±5.75 days. Flow cytometric analysis revealed that the cells negatively expressed CD34 and CD45 antigens while positively expressing CD44 and CD105 antigens. Differentiation into osteogenic and adipogenic lineage had taken place after one month culture in induction medium. VDR, COL1A1, BGLAP and SPARC gene expression indicated that mesenchymal stem cells isolated from canine bone marrow had differentiated into osteogenic lineage. These findings can form the basis of any forthcoming clinical studies involving the use of canine mesenchymal stem cells particularly in the field of bone and cartilage regeneration. Manuscript profile

Open Access Article

5 - Seroepidemiological survey of bovine leukemia virus infection in cows in Khuzestan province
سعید Zamanizadeh مهدی Pourmahdi Borujeni, M M.R Haji Hajikolaei M.R Seifi Abadshapouri

Bovine leukemia virus (BLV) is a member of the Delta retro virus genus (family Retroviridae) and can cause persistent lymphocytosis and lymphosarcoma in cattle that is described as enzootic bovine leucosis (EBL). This disease causes significant economic losses associate More

Bovine leukemia virus (BLV) is a member of the Delta retro virus genus (family Retroviridae) and can cause persistent lymphocytosis and lymphosarcoma in cattle that is described as enzootic bovine leucosis (EBL). This disease causes significant economic losses associated with the costs of control and eradication programs. Control programs of leucosis are based on the screening of cows by serological methods and removing the infected cows. The aim of this study was to evaluate the seroprevalence of bovine leukemia virus in cattle in Khuzestan province. Serum samples from 527 cattle were randomly collected in Ahvaz, Baghmalek, Shooshtar, Gotvand, Shadegan, Hendijan, Behbahan, Ramhormoz and Susangerd cities and were examined by ELISA assay. Seroperevalence rate of bovine leukemia virus was 6.64% (95% CI: 4.51-8.77). Statistical analysis indicated no significant association between infection and age or breed. Relative frequency of infection was higher in female cows than males, but this difference was not significant and odds of infection in female cows than males were 2.6 (95% CI: 0.35-19.59). Prevalence rate of infection between industrial (15%) and nonindustrial (3.4%) husbandry was significantly different (p<0.001) and odds of infection in industrial husbandry than nonindustrial was 4.97 (95% CI: 2.43-10.16). Prevalence rate in Shadegan, Baghmalek, Behbahan, and Susangerd were 23.5%, 20.3%, 7.2% and 1.7%, respectively, but infection was not observed in Ahvaz, Shooshtar, Gotvand, Ramhormoz and Hendijan (p<0.001) and 30.8% of fluctuation of disease was justified by geographical location. This study confirms that bovine leukemia virus exists in Khuzestan province. Prevention and control measures should be considered by health authorities. Manuscript profile

Open Access Article

6 - Survey of external parasites infestation of cyprinidea in Zarine-rud river in West Azerbaijan province
سهراب Rasouli سجاد Purghasem

In this research, external parasites infestation of carp fishes in Zarrineh-rud river, was examined. The species which were examined included capoeta, cyprinus carpio, carasius and ctenophoran. The fish were caught from summer 2012 to summer 2013 and their skin, fins an More

In this research, external parasites infestation of carp fishes in Zarrineh-rud river, was examined. The species which were examined included capoeta, cyprinus carpio, carasius and ctenophoran. The fish were caught from summer 2012 to summer 2013 and their skin, fins and gills were examined to detect external parasites. During the year a total of 480 fish were caught alive and transferred to the laboratory and examined following anesthesia and identification. Microscopic examinations were conducted on samples taken from the skin, fins and gills and the isolated parasites were studied under the light microscope (4x-100x magnification). Finally, collected data were analyzed by Excel,v.2013 and SPSS,v.19 and the student T-test was run to determine the relationship between the variables. Totally, seven different parasite species, including two species of protozoa, four monogenea and a crustacean were identified, all of which had direct life cycle (Dactylogyrus lenkorani, Dactylogyrus Iamellatus, Dactylogyrus extensus, Clinostomum complanatum, Ichthyophthirius multifiliis, Trichodina sp, Lernaea sp, Paradiplozoon sp.). According to the results, seventy percent of the fishes were infested by the mentioned species of parasites. Ichthyophthirius multifiliis was the most prevalent parasite (65.00%), whereas Paradiplozoon sp.1 was the least (0.54%), at p<0.05. The data analysis indicated that there is a statistically significant difference between different seasons (p<0.05). Also, there was a significant difference between the infested organs, with gills being the most infested organ (p<0.05).   Manuscript profile

Open Access Article

7 - Evaluation of cardiac injury biomarkers in sheep with acute lactic acidosis
مجید Fartashvand یعقوب Hajisadeghi

Rumen lactic acidosis is a metabolic disorder which develops in ruminants that have ingested large amounts of unaccustomed feeds rich in ruminally fermentable carbohydrates. In this study we investigated the relationship between serum lactate levels and ruminal fluid pH More

Rumen lactic acidosis is a metabolic disorder which develops in ruminants that have ingested large amounts of unaccustomed feeds rich in ruminally fermentable carbohydrates. In this study we investigated the relationship between serum lactate levels and ruminal fluid pH with changes of cardiac damage biomarkers in serum in 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy ones. After confirmation of ARLA through clinical examination and ruminal fluid pH£5.5, venous blood samples were collected and special analysis carried out on serum samples. According to the findings ruminal fluid pH in diseased and healthy sheep were 5.28 ± 0.2 and 6.93±0.3, respectively. Serum lactate level in sheep with ARLA was significantly higher than normal sheep. (p=0.000). cTnI levels was 0.684±0.03 ng/ml in sheep with ARLA, which was significantly (p=0.000) higher than healthy sheep (0.005±0.00 ng/ml). Other cardiac biomarkers were increased in diseased group, however only elevation of serum activities of AST and CK-MB were statistically significant (p=0.002 and p=0.007 respectively). Although serum LDH activity in diseased group was higher than control group; but this difference was statistically non-significant (p=0.063). There was significant negative correlation between ruminal fluid pH with cTnI concentrations (p=0.004; r=–0.850); so that highest levels of cTnI (2.28 to 3.06 ng/ml) were recorded in three sheep with ruminal fluid pH Manuscript profile

Open Access Article

8 - Study of cardiovascular biomarkers, cystatin-C and adenosine deaminase in alloxan-induced diabetes mellitus in the dog
کاوه Azimzadeh

Diabetes mellitus (DM) is a metabolic disease which may lead to considerable damages in most tissues. The purpose of this study was to assess whether the levels of serum Cardiac troponin I (cTnI), Homocysteine (Hcy), Cystatin c (Cys-C) and adenosine deaminase (ADA) acti More

Diabetes mellitus (DM) is a metabolic disease which may lead to considerable damages in most tissues. The purpose of this study was to assess whether the levels of serum Cardiac troponin I (cTnI), Homocysteine (Hcy), Cystatin c (Cys-C) and adenosine deaminase (ADA) activity are altered in alloxan induced diabetes mellitus in the dog. In this study, DM was induced by alloxan in the treatment group and three months later, plasma cTnI, Hcy and Cys-C were evaluated by Elisa technique and ADA activity was assessed by Electrochemilumminescence (ECL) method. The results showed significant increases (p<0.01) in cTnI and Cys-C along with a considerable decrease in ADA in the treatment group in comparison to the intact healthy group. Conclusively, increased plasma levels of cTnI and Cys-C in dogs with DM indicates heart and kidney damage respectively, which requires effective measures in the management of diabetes mellitus in dogs. Furthermore, reduction of ADA activity can be attributed to the decline of immune system (especially T cells), high levels of adenosine concentrations for facilitation of glucose transport and/or zinc ion decrease. Manuscript profile

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