Evaluation of cardiac injury biomarkers in sheep with acute lactic acidosis

Subject Areas : Veterinary Clinical Pathology

مجید  Fartashvand ¹ (Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
یعقوب  Hajisadeghi ² (Graduate of Veterinary Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)

Received: 2015-12-06 Accepted : 2016-06-30 Published : 2016-07-22

Keywords: cTnI, Cardiac damage, CK-MB, Acute lactic acidosis,

Abstract :

Rumen lactic acidosis is a metabolic disorder which develops in ruminants that have ingested large amounts of unaccustomed feeds rich in ruminally fermentable carbohydrates. In this study we investigated the relationship between serum lactate levels and ruminal fluid pH with changes of cardiac damage biomarkers in serum in 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy ones. After confirmation of ARLA through clinical examination and ruminal fluid pH£5.5, venous blood samples were collected and special analysis carried out on serum samples. According to the findings ruminal fluid pH in diseased and healthy sheep were 5.28 ± 0.2 and 6.93±0.3, respectively. Serum lactate level in sheep with ARLA was significantly higher than normal sheep. (p=0.000). cTnI levels was 0.684±0.03 ng/ml in sheep with ARLA, which was significantly (p=0.000) higher than healthy sheep (0.005±0.00 ng/ml). Other cardiac biomarkers were increased in diseased group, however only elevation of serum activities of AST and CK-MB were statistically significant (p=0.002 and p=0.007 respectively). Although serum LDH activity in diseased group was higher than control group; but this difference was statistically non-significant (p=0.063). There was significant negative correlation between ruminal fluid pH with cTnI concentrations (p=0.004; r=–0.850); so that highest levels of cTnI (2.28 to 3.06 ng/ml) were recorded in three sheep with ruminal fluid pH

References:

• پورجعفر، م.، محمدنیا، ا.، جعفری دهکردی ا. و فتاحیان دهکردی، ر. (1383). بررسی تجربی اثرات اسیدوز شکمبه بر الکترولیت­های سرم خون (سدیم، پتاسیم، کلسیم، فسفر)، هماتوکریت خون، نیتروژن اوره خون، گلوکز خون، pH شکمبه، میکروفلور شکمبه و ضایعات بافتی ایجاد شده در بافت شکمبه در گوسفند لری. فصلنامه پژوهش و سازندگی، شماره 4، پیاپی 62، صفحات: 36-27.


• نادعلیان، م.ق. (1381). بیماری­های دستگاه گوارش نشخوارکنندگان (بیماری­های حفره دهان، حلق، مری، پیش‌معده­ها و شیردان). انتشارات دانشگاه تهران، چاپ دوم، صفحات: 161-183.

• Aberra, A., Komukai, K., Howarth, F.C. and Orchard, C.H. (2001). The effect of acidosis on the ECG    of the rat heart. Experimental Physiology, 86: 27-31.


• Adams, J.E., Bodor, G.S., Davila-Roman, V.G., Delmez, J.A., Apple, F.S., Ladenson, J.H., et al. (1993). Cardiac troponin i a marker with high specificity for cardiac injury. Circulation, 88(1): 101-106.


• Ammann, P., Fehr, T., Minder, E.I., Gunter, C. and Bertel, O. (2001). Elevation of troponin I in sepsis and septic shock. Intensive Care Medicine, 27: 965-969.


• Anderson, R., Gardner, F.V., Honey, H., Noble, I.M. and Woodgate, D.W. (1968). Relation between metabolic acidosis and cardiac dysrhythmias in acute myocardial infarction. British Heart Journal, 30: 493.


• Babuin, L. and Jaffe. A.S. (2005). Troponin: the biomarker of choice for the detection of cardiac injury. CMAJ, 173(10): 1191-1202.


• Basak, D.M., Pan, S. and Chakrabarti, A. (1993). Physicochemical and microbial changes in rumen liquor of experimentaly induced lactic acidosis in goats. Indian Journal of Animal Sciences, 63(3): 263-267.


• Cao, G.R., English, P.B., Filippish, L.J. and Inglis, S. (1987). Experimental induced lactic acidosis in goat. Australian Veterinary Journal, 64(12): 367-370.


• Collinson, P.O. and Gaze, D.C. (2007). Biomarkers of cardiovascular damage and dysfunction-an overview. Heart, Lung and Circulation, 16: S71-S82.


• Coudrey, L. (1998). The Troponins. Archive International Medicine, 158(8): 1173-1180.


• Neaverson, M.A. (1966). Metabolic acidosis in acute myocardial infarction. British Medical Journal, 2: 383-385.


• Jafari-Dehkordi, A., Haji-Hajikolaei, M.R. and Karimi-Dehkord, Z. (2011). ECG Changes in Acute Experimental Ruminal Lactic Acidosis in Sheep. Veterinary Research Forum, 2(3): 203-208.


• Lean, I.J., Wade, L.K. and Porter, J. (2000). New approaches to control of ruminal acidosis in dairy cattle. Asian-Australasian Journal of Animal Sciences, 13(Suppl): 266-269.


• Lean, I.J., Annison, F., Bramley, E., Browning, G., Cusack, P., Farquharson, B., et al. (2007). Ruminal Acidosis– understandings, prevention and treatment. Australian Veterinary Association, 3-46.


• Lott, J.A. and Stang, J.M. (1980). Serum enzymes and isoenzymes in the diagnosis and differential diagnosis of myocardial ischemia and necrosis. Clinical Chemistry, 26: 1241-1250.


• Mehtaa, N.J., Khana, I.A., Gupta, V., Jani, K., Gowda, R.M. and Smith, P.R. (2004). Cardiac troponin I predicts myocardial dysfunction and adverse outcome in septic shock. International Journal of Cardiology, 95: 13-17.


• Nocek, J.E. (1997). Bovine Acidosis: Implications on Laminitis. Journal of Dairy Science, 80(5): 1005-1028.


• Nour, M.S.M., Abusamara, M.T. and Hago B.E.D. (1999). Experimentally induced lactic acidosis in Nubian goats clinical, biochemical and pathological investigation. Small Ruminant Research, 31(1): 7-17.


• Orchard, C.H., Cingolani, H.E. (1994). Acidosis and arrhythmias in cardiac muscle. Cardiovascular Research, 28: 1312.


• Ortolani, E.L. (1995). Induction of lactic acidosis in cattle with sucrose relationship between dose, rumen fluid pH and animal size. Veterinary and Human Toxicology, 37(5): 462-464.


• Parmacek, M.S. and Solaro, R.J. (2004). Biology of the troponin complex in cardiac myocytes. Progress in Cardiovascular Diseases, 47(3): 159-176.


• Patra, R.C. Lal, S.B. and Swarup, D. (1993). Physicochemical alterations in blood, cerebrospinal fluid and urine in experimental lactic acidosis in sheep. Research in Veterinary Science, 54: 217-220.


• Peek, S.F., Apple, F.S., Murakami, M.A., Crump, P.M. and Sem-Rad, S.D. (2008). Cardiac isoenzymes in healthy Holstein calves and calves with experimentally induced endotoxemia. Canadian Journal of Veterinary Research, 72: 356-361.


• Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. (2007). Veterinary Medicine, a text book of the disease of cattle, sheep, pigs and horses. 10th ed., Elsevier Publishing, pp: 314-325.


• Sharma, S., Jackson, P.G. and Makan, J. (2004). Cardiac troponins. Journal of Clinical Pathology, 57: 1025-1026.


• Tunca, R., Erdoğan, H.M., Sözmen, M., Çitil, M., Devrim, A.K., Erginsoy, S., et al. (2009). Evaluation of cardiac troponin I and Inducible nitric oxide synthase expressions in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences, 33: 53-59.


• Varga, A., Schober, K.E., Walker, W.L., Lakritz, J. and Michael D. (2009). Validation of a commercially available immunoassay for the measurement of bovine cardiac troponin I. Journal of Veterinary Internal Medicine, 23: 359-365.


• Wendy, J.U. (1992). Rumen lactic acidosis. Part II. Clinical signs, diagnosis, treatment, and prevention. Continuing Education Article (Compendium); 14(9): 1265-1270.