The effect of combination of probiotic, lactase enzyme and special antibodies on growth factors and histomorphology of small intestine in broiler chickens
Subject Areas :
Veterinary Clinical Pathology
Mir Hamed Sarvgad
1
,
Afshin Zakeri
2
1 - Graduate of Animal Nutrition, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Assistant Professor, Department of Animal Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Received: 2017-04-05
Accepted : 2017-07-11
Published : 2018-04-21
Keywords:
broiler chickens,
Probiotic,
Small intestine,
Lactase enzyme,
Growth factor,
Abstract :
Improvement of the immune system response along with increase in performance and growth factors are important goals of the modern poultry industry. The aim of this study was to determine the effect of combination of probiotic, lactase enzyme and special antibodies on improving the small intestinal histomorphology and growth factors in broiler chickens. For this purpose, 96 broilers (Ross 308) were distributed randomly into two groups of 48 with 4 replicates of 12 chicks. In the experimental group, two grams of the test compound was used in water for 42 days. All birds were weighed at the end of each week in order to investigate their growth factors. For histomorphological characterization, two broilers from each replicate were selected and the entire small intestine was removed and sent to the histology lab. The results indicated a significant difference in growth factors between the two groups from the third to the sixth week (p<0.01). Crypt depth, villi height, thickness of muscular and mucosal layers and the number of goblet cells in different parts of the intestine was significantly different between the two groups (p<0.01). The results showed that this compound can improve intestinal histomorphology and increase nutrient absorption and efficiency of food and improve growth factors therefore it seems that its use can improve the standard of growth in the poultry industry.
References:
Aliakbarpour, H., Karimi Tarshizy, M., Rezaeian, M., Kelarikelaei, K. and Dozori, R. (2015). Effect of probiotics in broiler diets on growth, immune system organs and small intestinal morphology in the first week of age. Journal of Veterinary Research, 60(3): 92-98. [In Persian]
Chichlowski, M.J., Croom, B.W., Mc Bride, L., Danile, G.D. and Kaci, D.C. (2007). Direct fed microbial primalc and sally nomay cine modulate whole body and intestinal oxygen consumption and intestinal mucosal cytokine production in the broiler chick. Poultry Science, 86(2): 1100-1106.
Jafari Ahangari, Y., Parizadian Kavan, B. and Hoseini Zadeh, M. (2010). Effect of probiotics on performance and safety parameters of broiler chickens. Journal of Research in Livestock Production, 8(2): 3-8. [In Persian]
Kabir, M.L., Rahman, M.B., Rahman, M.M. and Ahmed, S.U. (2004). The dynamics of probiotics on growth performance and immune response in broilers. Poultry Science, 3(4): 361-364.
Karimi, K. and Rahimi, Sh. (2003). Effect of different levels of Probiotics on performance of broiler chickens. Journal of Research and Development in Cattle Breeding and Aquaculture, 60(2): 19-24. [In Persian]
Khaksefifi, A. and Ghoorchi, T. (2006). Effect of probiotic on performance and immune competence in broiler chicks. Poultry Science, 43(3): 296-300.
Laudadio, V., Passantino, L., perillo, A., Lopresti, G. and Passantino, A. (2011). Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poultry Science, 91(1): 265-270.
Mir Babaei Langerodi, N., Mohammadi, M. and Rostaei Ali Mehr, M. (2012). Effect of Probiotic protexin and formic acid on performance of broiler chickens. Journal of Research in Livestock Production, 38(4): 380-386. [In Persian]
Mortazavian, A. and Sohrab Vandi, S. (2006). Probiotics and Probiotic Food Products. Iran: Tehran, Ata Publication, pp: 20-76. [In Persian]
Sucio, I., Miclea, I. and Lonzone, T.D. (1990). Effect of replacing part of the protein in the diet with urea, in the present of volcanic tuff zeolite, on the performance of broiler chickens. Buletinul Institutului Agronomic, 44(2): 33-18.
Teshfam, M., Rahimi, S.H. and Karimi, K. (2005). Effect of probiotics on intestinal mucosal morphology of broiler chickens. Journal of Veterinary Research, 24(2): 18-23. [In Persian]
Zakia, A., Mahmed, M., Zahraa, H. and Ghamdi, E.L. (2008). Multiple environmental stresses and broiler internal organs somatic indices under controlled environment. International Journal of Poultry Science, 7(4): 1089-1094.
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Aliakbarpour, H., Karimi Tarshizy, M., Rezaeian, M., Kelarikelaei, K. and Dozori, R. (2015). Effect of probiotics in broiler diets on growth, immune system organs and small intestinal morphology in the first week of age. Journal of Veterinary Research, 60(3): 92-98. [In Persian]
Chichlowski, M.J., Croom, B.W., Mc Bride, L., Danile, G.D. and Kaci, D.C. (2007). Direct fed microbial primalc and sally nomay cine modulate whole body and intestinal oxygen consumption and intestinal mucosal cytokine production in the broiler chick. Poultry Science, 86(2): 1100-1106.
Jafari Ahangari, Y., Parizadian Kavan, B. and Hoseini Zadeh, M. (2010). Effect of probiotics on performance and safety parameters of broiler chickens. Journal of Research in Livestock Production, 8(2): 3-8. [In Persian]
Kabir, M.L., Rahman, M.B., Rahman, M.M. and Ahmed, S.U. (2004). The dynamics of probiotics on growth performance and immune response in broilers. Poultry Science, 3(4): 361-364.
Karimi, K. and Rahimi, Sh. (2003). Effect of different levels of Probiotics on performance of broiler chickens. Journal of Research and Development in Cattle Breeding and Aquaculture, 60(2): 19-24. [In Persian]
Khaksefifi, A. and Ghoorchi, T. (2006). Effect of probiotic on performance and immune competence in broiler chicks. Poultry Science, 43(3): 296-300.
Laudadio, V., Passantino, L., perillo, A., Lopresti, G. and Passantino, A. (2011). Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poultry Science, 91(1): 265-270.
Mir Babaei Langerodi, N., Mohammadi, M. and Rostaei Ali Mehr, M. (2012). Effect of Probiotic protexin and formic acid on performance of broiler chickens. Journal of Research in Livestock Production, 38(4): 380-386. [In Persian]
Mortazavian, A. and Sohrab Vandi, S. (2006). Probiotics and Probiotic Food Products. Iran: Tehran, Ata Publication, pp: 20-76. [In Persian]
Sucio, I., Miclea, I. and Lonzone, T.D. (1990). Effect of replacing part of the protein in the diet with urea, in the present of volcanic tuff zeolite, on the performance of broiler chickens. Buletinul Institutului Agronomic, 44(2): 33-18.
Teshfam, M., Rahimi, S.H. and Karimi, K. (2005). Effect of probiotics on intestinal mucosal morphology of broiler chickens. Journal of Veterinary Research, 24(2): 18-23. [In Persian]
Zakia, A., Mahmed, M., Zahraa, H. and Ghamdi, E.L. (2008). Multiple environmental stresses and broiler internal organs somatic indices under controlled environment. International Journal of Poultry Science, 7(4): 1089-1094.