Molecular characterization of quinolone resistance genes (qnr) in Salmonella ‎typhimurium isolated from food samples

Subject Areas : Food Science and Technology

K. Amini ¹ , T. Ghaseminezhad Raeini ² , B. Kheirkhah ³

1 - Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
2 - Department of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran
3 - Assistant Professor, Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran

Received: 2016-10-12 Accepted : 2017-01-04 Published : 2017-12-22

Keywords: Salmonella typhimurium, Multiplex-PCR, qnr genes.&lrm, ,

Abstract :

Salmonellosis is an important disease in animals and human which is caused by different serovars of Salmonella ‎enterica. Serovars Typhimurium is one of the most prevalent sorvars in humans. Quinolones and floroquinolones ‎are the family of extended-spectrum antibiotics which are used in salmonellosis treatment. Qnr genes are the ‎plasmid-mediated quinolones resistance which leads to resistance in Enterobacteiacea. The aim of this study is ‎identification of quinolone resistance genes qnr in Salmonella Typhimurium isolated from food samples. In this ‎study, 60 Salmonella samples isolated from food was collected and confirmed by culture and biochemical tests. ‎Serotyping was done by O and H antisera. Multiplex-PCR‏ ‏‎ was performed to identify qnrA, qnrB and qnrS genes. ‎All of the 60 isolates were confirmed as Salmonella by culture and biochemical tests. The results of serotyping ‎showed all the 60 isolates were belonged to serogroup B and serovar Typhimurium. Multiplex-PCR test showed 5 ‎samples had the qnrB, 4 had the qnrS and 1 harboured qnrA gene. The results of this study shows the presence of ‎qnr genes in Salmonella Typhimurium isolated from food samples which has a specific public health importance. ‎Therefore, there should be sureveillance and montoring programs to prevent this quinolone resistance.‎

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