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  1 - Molecular characterization of quinolone resistance genes (qnr) in Salmonella ‎typhimurium isolated from food samples
  K. Amini T. Ghaseminezhad Raeini B. Kheirkhah
  Salmonellosis is an important disease in animals and human which is caused by different serovars of Salmonella ‎enterica. Serovars Typhimurium is one of the most prevalent sorvars in humans. Quinolones and floroquinolones ‎are the family of extended-spectrum ant More

  Salmonellosis is an important disease in animals and human which is caused by different serovars of Salmonella ‎enterica. Serovars Typhimurium is one of the most prevalent sorvars in humans. Quinolones and floroquinolones ‎are the family of extended-spectrum antibiotics which are used in salmonellosis treatment. Qnr genes are the ‎plasmid-mediated quinolones resistance which leads to resistance in Enterobacteiacea. The aim of this study is ‎identification of quinolone resistance genes qnr in Salmonella Typhimurium isolated from food samples. In this ‎study, 60 Salmonella samples isolated from food was collected and confirmed by culture and biochemical tests. ‎Serotyping was done by O and H antisera. Multiplex-PCR‏ ‏‎ was performed to identify qnrA, qnrB and qnrS genes. ‎All of the 60 isolates were confirmed as Salmonella by culture and biochemical tests. The results of serotyping ‎showed all the 60 isolates were belonged to serogroup B and serovar Typhimurium. Multiplex-PCR test showed 5 ‎samples had the qnrB, 4 had the qnrS and 1 harboured qnrA gene. The results of this study shows the presence of ‎qnr genes in Salmonella Typhimurium isolated from food samples which has a specific public health importance. ‎Therefore, there should be sureveillance and montoring programs to prevent this quinolone resistance.‎ Manuscript profile