The effect of long-term endurance training on serum levels of adiponectin and its expression in subcutaneous fat tissue of obese diabetic rats
Subject Areas : New studies in exercise metabolism and physical activity
mahboobeh pak zadian
1
,
mohammad rashidi
2
1 - Department of Physical Education and Sports Sciences, Semnan Branch, Islamic Azad University, Semnan, Iran
2 - Dept. of exercise physiology, semnan branch, islamic Azad university, semnan, Iran
Keywords: Aerobic exercise, Gene expression, Insulin resistance, Glucose homeostasis, Type 2 diabetes,
Abstract :
Objective: The present experimental study was conducted with the aim of determining the effect of 10 weeks of endurance training on serum levels and expression of adiponectin in subcutaneous adipose tissue, as well as glucose and insulin resistance in type 2 diabetic rats. Matherials and Method: The statistical population consisted of all male Wistar rats of Baqiyatullah University of Medical Sciences, from which 14 rats weighing 220±10 g were purchased. In the following, the studied rats were subjected to the effect of 8 weeks of high-fat diet and intraperitoneal injection of STZ type 2 diabetes and were randomly divided into two groups (control and aerobic). Then the aerobic group participated in an aerobic (endurance) training for 10 weeks with 5 sessions per week. Finally, 48 hours after the last training session, the fasting levels of glucose, serum adiponectin, insulin resistance and adiponectin expression in the subcutaneous adipose tissue of both groups were measured and compared by independent t-test. Results: Aerobic training led to a significant decrease in fasting glucose and insulin resistance and a significant increase in the expression of adiponectin in subcutaneous fat tissue compared to the control group. However, serum adiponectin did not change significantly in response to aerobic exercise. Conclusion: Based on these findings, the improvement of blood glucose and insulin resistance in rats in the aerobic group compared to the control group is probably rooted in the increased expression of adiponectin in the subcutaneous fat tissue in response to aerobic exercise. However, understanding the mechanisms responsible for improving glucose homeostasis in response to exercise training requires the measurement of other genetic and hormonal components.
