Evaluation of human sperm parameters after cryopreservation process with myoinositol antioxidant treatment in asthenoteratozoospermia patients

Subject Areas :

rahil jannatifar ¹ , hamid piroozmanesh ² , lila naserpoor ³

1 - Department of Reproductive Biology,Academic Center for Education,Culture and Research(ACECR),Qom,Iran
2 - Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran.
3 - Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran

Received: 2021-09-27 Accepted : 2021-11-13 Published : 2022-02-20

Keywords: cryopreservation, Asthenoteratozoospermia, Antioxidant, Myoinositol,

Abstract :

Inroduction & Objective: Myoinositol, which is one of the components of cell membranes with its strong antioxidant properties, has a high potential to neutralize the damage caused by freezing. The aim of this study was to evaluate the effect of Myoinositol antioxidant on sperm sperm parameters after cryopreservation process Material and Methods: Samples of 20 patients with asthenotratospermia referred to Qom University Jihad Infertility Treatment Center were evaluated in three groups: before cryopreservation (control), cryopreservation, cryopreservation + myoinositol. The Sperm parameters such as motility, morphology, and viability were assessed using WHO, Papanicolaou, and eosin-necrosin staining, respectively, to evaluate the integrity of the plasma membrane using the HOST technique, as well as to evaluate the degree of DNA fracture by the Halo sperm technique. Results: The results showed that incubation of myoinositol with freezing medium of sperm (cryopreservation + myoinositol) increases sperm motility and viability (P <0.05). Also, a significant increase in sperm motility was achieved after incubation of myoinositol with sperm sample (cryopreservation + myoinositol) (P <0.05). The results of the present study showed that incubation of myoinositol with freezing medium of sperm significantly reduces the amount of DNA damage (P <0.05). Plasma membrane integrity and sperm mitochondrial membrane potential also increased significantly in cryopreservation + myoinositol group (P <0.05). However, sperm morphology did not show a significant difference (P <0.05). Conclusion: Our results show that my inositol can reduce the destructive effects of the freeze-thaw process.

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