Detection of extended-spectrum beta-lactamase (ESBL) producing genes in Escherichia coli strains isolated from dead canaries in Isfahan
Subject Areas :
دانیال گل کاریان
1
,
مجید غلامی آهنگران
2
,
آسیه احمدی دستگردی
3
1 - دانش آموخته دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
2 - بیماریهای طیور،دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی
3 - گروه علوم و صنایع غذایی، واحد اردستان، دانشگاه آزاد اسلامی، اردستان، ایران.
Keywords: Escherichia coli, beta-lactamase, canary, antibiotic resistance,
Abstract :
In this study, 100 Escherichia coli (E. coli) strains isolated from dead canaries and 22 E. coli strains isolated from the cloacal swabs of apparently healthy canaries were studied. After confirming E. coli strains with microbial and biochemical tests, the resistance of the strains to ceftazidime and cefotaxime was determined, and ESBL-producing E. coli strains were identified using discs combined with clavulanic acid. Beta-lactamase producing strains were investigated for blaTEM, blaCTX and blaSHV genes. The results showed that out of a total of 100 E. coli strains isolated from dead canaries, 20 strains are phenotypically able to produce beta-lactamase. Out of 20 beta-lactamase producing E. coli strains, 13 strains are able to replicate genomic fragments containing blaTEM, blaCTX and blaSHV genes. The most β-lactamase gene detected in both groups of canaries is blaCTX type, and blaSHV gene was not detected in any of the canaries. In the isolated Escherichia coli strains, the percentage of resistance to ceftazidime and cefotaxime antibiotics is related to the detection of the blaCTX gene, so that all the resistant strains had the blaCTX gene. Considering the prevalence of beta-lactamase producing strains in Escherichia coli isolated from dead canaries and the possibility of transferring these genes to other bacteria along with other antibiotic resistance genes, it is necessary to treat pet birds with permitted antibiotics and with compliance dose and duration of use. In general, it seems that the incorrect use of broad-spectrum antibiotics can be the origin of the formation and spread of beta-lactamase-producing strains in pet birds.
. Acikgoz, Z.C., Koseoglu, E.O., and Kocagoz, S. (2007). CTX-M-3 Beta lactamase produsing Shigella Sonnei isolated from pediatric Bacillary Dysentry cases. Japanese Journal of Infectious Diseases, 61 135-137.
2. Akond, M. (2009). Antibiotic Resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. American Journal of Environmental Sciences, 5: 47–52.
3. Argudín, M., Deplano, A., Meghraoui, A., Dodémont, M., Heinrichs, A., Denis, O. and Roisin, S. (2017). Bacteria from animals as a pool of antimicrobial resistance genes. Antibiotic, 6: 12.
4. Beleza, AJ., Maciel, WC., Carreira, AS., Bezerra, WG., Carmo, CC., Havt, A., Gaio, FC and Teixeira, RS (2019). Detection of Enterobacteriaceae, antimicrobial susceptibility, and virulence genes of Escherichia coli in canaries (Serinus canaria) in northeastern Brazil. Pesquisa Veterinária Brasileira, 39: 201-208.
5. Costa, D., Poeta, P., Sáenz, Y., Vinué, L., Rojo-Bezares, B., Jouini, A., and Torres, C. (2006). Detection of Escherichia coli harbouring extended-spectrum β-lactamases of the CTX-M, TEM and SHV classes in faecal samples of wild animals in Portugal. Journal of Antimicrobial Chemotherapy, 58: 1311-1312.
6. Costa D., Vinue L., Poeta P., Coelho, AC., Matos, M., Sáenz, Y and Torres, C. (2009). Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in fecal samples of broilers. Veterinary Microbiology, 138: 339-344.
7. Damborg, P., Broens, E.M., Chomel, B.B., Guenther, S., Pasmans, F., Wagenaar, J.A. and Guardabassi, L. (2016). Bacterial zoonoses transmitted by household pets: state of the art and future perspectives for targeted research and policy actions. Journal of Comparative Pathology, 155: 27–40.
8. Dierikx, C., Van der Goot, J., Fabri, T., van Essen-Zandbergen, A., Smith, H and Mevius, D. (2013). Extended-spectrum β-lactamase and Ampc β-lactamase-producing Escherichia coli in Dutch broilers and broiler farmers. Journal of Antimicrobial Chemotherapy, 68: 60-67.
9. Feng, P., Weagant, S., and Grant, M. (2002). Bacteriological Analytical Manual. 8th edition, US FDA Centre for Food Safety and Applied Nutrition Publishing, Maryland, USA.
10. Gaio, F.C. (2017). Isolamento, resistência e caracterização das cepas de Escherichia coli e Salmonella spp. em Psitacídeos presentes no centro de triagem de animais silvestres do Ceará. Master's Thesis, Postgraduate Program in Veterinary Science, Universidade Estadual do Ceará, Fortaleza, CE. 57p.
11. Gholami-Ahangaran, M., and Zia-Jahromi N. (2014). Identification of shiga toxin and intimin genes in Escherichia coli detected from canary (Serinus canaria domestica). Toxicology and Industrial Health, 30: 724-727.
12. Gholami-Ahangaran, M., Moravvej, A. H., Safizadeh, Z., Nogoorani, V. S., Zokaei, M., and Ghasemian, S. O. (2021). The evaluation of ESBL genes and antibiotic resistance rate in Escherichia coli strains isolated from meat and intestinal contents of turkey in Isfahan, Iran. Iranian Journal of Veterinary Research, 22: 318.
13. Gholami-Ahangaran, M., Karimi-Dehkordi, M., Miranzadeh-Mahabadi, E., and Ahmadi-Dastgerdi, A. (2021). The frequency of tetracycline resistance genes in Escherichia coli strains isolated from healthy and diarrheic pet birds. Iranian Journal of Veterinary Research, 22: 337.
14. Gholami Ahangaran, M., Zinsaz, P., Pourmahdi, O., Ahmadi-Dastgerdi, A., Ostadpour, M., and Soltani, M. (2022). Tetracycline Resistance Genes in Escherichia coli Strains Isolated from Biofilm of Drinking Water System in Poultry Farms. Acta Veterinaria Eurasia, 48: 64-69.
15. Giacopello, C., Foti, M., Fisichella, V. and Piccolo, F.L. (2015). Antibiotic-resistance patterns of Gram-negative bacterial isolates from breeder canaries (Serinus canaria domestica) with clinical disease. Journal of Exotic Pet Medicine, 24: 84–91.
16. Girlich, D., Poirel, L., Carattoli, A., Kempf, I., Lartigue, M. F., Bertini, A., and Nordmann, P. (2007). Extended-spectrum β-lactamase CTX-M-1 in Escherichia coli isolates from healthy poultry in France. Applied and Environmental Microbiology, 73: 4681-4685.
17. Guenther, S., Grobbel, M., Beutlich, J., Bethe, A., Friedrich, N. D., Goedecke, A. and Ewers, C. (2010). CTX‐M‐15‐type extended‐spectrum beta‐lactamases‐producing Escherichia coli from wild birds in Germany. Environmental microbiology reports, 2: 641-645.
18. Hidasi, HW., Neto, JH and Moraes, DM (2013). Enterobacterial detection and E. coli antimicrobial resistance in parrots seized from the illegal wildlife trade. Journal of Avian Medicine Surgery, 27: 166-167.
19. Horn, RV., Cardoso, WM., Lopes, ES., Teixeira, RS., Albuquerque, ÁH., Rocha-e-Silva, RC., Machado, DN and Bezerra, WG (2015). Identification and antimicrobial resistance of members from the Enterobacteriaceae family isolated from canaries (Serinus canaria). Pesquisa Veterinária Brasileira, 35: 552-556.
20. Jafari, RA., Motamedi, H., Maleki, E., Ghanbarpour, R and Mayahi, M (2016). Phylogenetic typing and detection of extended-spectrum β-lactamases in Escherichia coli isolates from broiler chickens in Ahvaz., Iran. Veterinary Research Forum, 7: 227-232.
21. Junyoung, K., Semi, J., Hogeun, R., Bokkwon, L., Misun, P., Hoanjong, L., Jina, L., and Seonghan, K. (2009). Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method. Infection and Chemotherapy, 41, 181-184.
22. Nijsten, R., London, N., Van Den Bogaard, A. and Stobberingh, E (1996). In-vitro transfer of antibiotic resistance between faecal Escherichia coli strains isolated from pig farmers and pigs. Journal of Antimicrobial Chemotherappy, 37: 1141-1154.
23. Nolan, L.K., Barnes, H.J., Vaillancourt, J.P., Abdul-Aziz, T., and Logue, C.M. (2013). Colibacillosis. In: Swayne, D.E., Glisson, J.R., McDougald, L.R., Venugopal, N., Nolan, L. (eds.), Disease of Poultry. 13th edition, Wiley-Blackwell, Massachusetts, pp. 751-807.
24. Paterson, D. L., and Bonomo, R. A. (2005). Extended-spectrum β-lactamases: a clinical update. Clinical microbiology reviews, 18: 657-686.
25. Radhouani, H., Pinto, L., Coelho, C., Gonçalves, A., Sargo, R., Torres, C. and Poeta, P. (2010). Detection of Escherichia coli harbouring extended-spectrum b-lactamases of the CTX-M classes in faecal samples of common buzzards (Buteo buteo). Journal of Antimicrobial Chemotherappy, 65: 171-3.
26. Reich, F., Atanassova, V and Klein, G (2013). Extended-spectrum β-lactamase and Ampc-producing Enterobacteria in healthy broiler chickens, Germany. Emerging Infectious Diseases, 19: 1253-1259.
27. Shaheen B.W., Nayak R., Foley S.L. (2011). Molecular characterization of resistance to extended-spectrum cephalosporins in clinical Escherichia coli isolates from companion animals in the United States. Antimicrobial Agents and Chemotherapy, 55:5666-5675.
28. Singh, S. (1999). Extended-Spectrum beta-lactamases: An overview. Diagnostic Laboratory Services. INC.
29. Smet, A., Martel, A., Persoons, D., Dewulf, J., Heyndrickx, M., Catry, B. and Butaye, P (2008). Diversity of extended-spectrum β-lactamases and class C β-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms. Antimicrobial Agents and Chemotherapy, 52: 1238-1243.
30. Toombs-Ruane, L. J., Benschop, J., French, N. P., Biggs, P. J., Midwinter, A. C., Marshall, J. C., and Douwes, J. (2020). Carriage of Extended-Spectrum-Beta-Lactamase-and AmpC Beta-Lactamase-Producing Escherichia coli Strains from Humans and Pets in the Same Households. Applied and Environmental Microbiology, 86, 100-111.
31. Van den Bogaard, A.E., London, N. and Driessen, C.A.G.G. and Stobberingh, EE (2001). Antibiotic resistance of fecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of Antimicrobial Chemotherappy, 47: 763-771.
32. Yazdi, M., Nazemi, A., Mirinargasi, M., Khataminejad, M.R., Sharifi, S., and Babaikochkaksaraei, M. (2010). Prevalence of SHV/CTXM/ TEM (ESBL) Beta-lactamase Resistance Genes in Escherichia coli Isolated from Urinary Tract Infections in Tehran, Iran. Medical Laboratory Journal, 4: 67-74.
33. Yılmaz, E. Ş., and Dolar, A. (2017). Detection of extended-spectrum β-lactamases in Escherichia coli from cage birds. Journal of Exotic Pet Medicine, 26: 13-18
