Comparison of the Effect of Eight Weeks of Aerobic and Resistance Training with Quercetin Supplementation on Vascular Endothelial Growth Factor (VEGF) in Cardiac Tissue of Male Wistar Rats
Subject Areas : MicrobiologyM. Kashef 1 , F. Bahadori 2 , F. Shahidi 3
1 - Professor of Shahid Rajaee Teacher Training University, Tehran, Iran.
2 - MSc Student of Nutritional Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran.
3 - Associate Professor of Shahid Rajaee Teacher Training University, Tehran, Iran.
Keywords: Exercise, Quercetin, Rat, Vascular Endothelial Growth Fa,
Abstract :
Introduction: Exercise has an effective role in physical and mental health by reducing the health risk factors. Materials and Methods: The study aimed to compare the effect of eight weeks of aerobic and resistance training with quercetin supplementation on VEGF of cardiac tissue in rats. 35 Rats with an age average of 8-10 weeks were randomly divided into 7 groups (aerobic, resistance, aerobic + supplement, resistance + supplement, supplement, sham, and control). After two weeks of familiarity with the environment and learning the exercise, The Exercise groups practiced according to the protocol for 8 weeks. The groups that should have received supplements received quercetin supplement at the rate of 50 mg/kg three days a week for 8 weeks as a peritoneal injection. 48 hours after the last training intervention, rats were anesthetized and killed using CO2. The, samples were taken from the heart of rats. ELISA method was used to measure VEGF concentration. One-way ANOVA and Tukey Post Hoc test in P≤0.05 level by SPSS 22 was employed. Results: The results showed that eight weeks of aerobic and resistance training and aerobic with quercetin significantly increased the VEGF concentration of rat heart compared to the control group (P≤0.05), while, there was no significant difference in the level of VEGF in heart tissue compared to the control group and supplement (P=0.148). Conclusion: In general, quercetin consumption inhibited angiogenesis, while, it did not happen in the supplement + aerobic group (P=0.011), that means that aerobic has overcome the inhibitory effects of quercetin on angiogenesis.
Arts, M., Sebastiaan, J., Voss, H. P., Haenen, G. & Bast, A. (2004). A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chemistry, 88(4), 567-570.
Azizi, F., Saadat, N., Salehi, P. & Emami, H.
(2001). The relationship between glucose intolerance and blood pressure, body mass index and waist to hip ratio in Tehran urban population (Tehran Lipid and Glucose Study). Iranian Journal of Endocrinology and Metabolism, 3(4), 256-247 [In Persian].
Bedford, T.G. (1979). Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology, 47(6), 1278-1283.
Bobik, A. (2005). The structural Basis of hypertwnsion vascular remodelling, rare faction and angiogenesis /arteriogenesis. Journal of Hypertension, 23,1473–1475.
Erekat, N. S., Al-Jarrah, M. D. & Khatib, A. J. (2017). Treadmill exercise training improves vascular growth factor expression in the cardiac muscle of TypeI diabetic rats. Cardiol.
Gavin, T., Drew, J., Kubik, C., Pofahl, W. & Hickner, R. (2007). Acute resistance exercise increases skeletal muscle angiogenic growth factor expression. Acta Physiology, (2)191, 46-139.
Goktepe, M. & Gunay, M. (2014). The effect of quercetin administration on exercise, Free Radical and Antioxidant Enzym Levels; 2 (5), 775-788.
Gordaliza, M. (2007). Natural products as leads to anticancer drugs. Clin Transl Oncol 9: 767-776.
Leandro, C. J. (2007). A program of moderate physical training for Wistar rats based on maximal oxygen consumption. Journal of Strength and Conditioning Research, 21(3), 751.
Lee, S. (2004). Viral expression of insulin- like growth factor-l enhances muscle, hypertrophy in resistance – training rats. Journal of Applied Physiology, 96(3), 1097-1104.
Mahrou, M., Gaeini, A., Chobbineh, S. & Javidi, M. (2014). Changes in simulating factors of angiogenesis, induced by progressive resistance training diabetic rats. Iranian journal of Diabetes and Metabolism, 14(1), 2-8 [In Persian].
Molla Hasanzadeh, F., Bijeh, N. & Moazami, M. (2017). The effect of eight weeks of aerobic exercise on angiogenesis and body composition in overweight women. Sport physiology, 8(29), 56-32 [In Persian].
Moradi, E., Heydarian, E., Gholami, M., Saffari, J., Mardani, G., Karimi, A. &Nourmohammadi, Z. (2015). Effect of Quercetin on Methotrexate-induced Hepatic and Renal Damages in Male Rats. Journal Mazandaran University Medical Science, 25 (127), 25-37 [In Persian].
Noorshahi, M., Emamian Rostami, M. & Khodagholi, F. (2017). The effect of 8 weeks of intermittent speed training on VEGF levels in skeletal muscle tissue of elderly rats. Medical Journal of Tabriz University of Medical Sciences and Health Services, 40 (2), 94-88 [In Persian].
Nourshahi, M., Farahmand, F. & Bigdeli, M. R. (2017). Effect of cinnamon-extract supplementation on VEGF and Endostatin level in hind leg muscle of aged rats after one session of exhaustive exercise. Iran Journal of Physiology Pharmacology., 1(3), 176-184 [In Persian].
Paczek, B., Bartlomiejczyk, I. & Przybylski, J. (2006). The serum levels of growth factors: PDGF, TGF-BET and VEGF are induced after strenuous physical exercise. Journal of Physiology Pharmacology, 191-197.
Roskoski, R. (2008). VEGF receptor protein–tyrosine kinases: Structure and regulation. Biochemical and Biophysical Research Communications, 3, 287–291.
Soori, R., Sharafi Dehrahm, F., Choobineh, S., Valipour Dehnou, V. (2018). Effect of endurance training on VEGF protein level in tissue of cardiac muscle in STZ-induced diabetic Wistar rats. Journal of Medical Sciences of Lorestan University (Yafteh), 20(3), 110-124 [In Persian].
Taheri Chadernshin, H., Noorshahi, M., Ranjbar, K. (2010). The response of vascular endothelial growth factor to exhausted submaximal exercise and its relationship with VO2max. Journal of Sport Biosciences, 7, 75-59 [In Persian].
Tang, K., Xia, F. C., WagneSr, P. D. & Breen, E.C. (2010). Exercise – induced VEGF transcriptional activitation in brain, lung and skeletal muscle. Respiratory Physiology & Neurobiology, 170, 16-22.
Tiago, F., Baraúna, V. G., Negrão, C. E., Phillips, M. I. & Oliveira, E. M. (2015). Aerobic exercise training, 80(3), 35-50.
Urso, M. L. & Clarkson, P. M. (2003). Oxidative stress, exercise and antioxidant supplementation. Toxicol, 189 (1-2), 41 - 54.
Ushio Fukai, M. & Yoshimasa, N. (2008). Reactive oxygen species and angiogenesis: NADPH oxidase as target for cancer therapy. Cancer Lett, 266, 37–52.
Uysal, N. (2015). Effects of voluntary and involuntary exercise on cognitive functions, and VEGF and BDNF levels in adolescent rats. Biotechnic & Histochemistry, 90 (1), 55-68.
Vali Zadeh, S., Motamedi, P., Karami, H. & Hamid, Rajabi. (2018). The Effects of Endurance Training on Gene Expression of VEGF and VEGFR2 of Cardiac Tissue in Type 2 Diabetic Male Wistar. Journal of Arak University of Medical Sciences, 21(6), 108-117 [In Persian].
Werner, G. S., Ferrari, M., Betge, S., Gastmann, O., Richartz, B. M. & Figulla, H. R. (2001). Collateral function in chronic total coronary occlusions is related to regional myocardial function and duration of occlusion. Circulation, 104(23), 2784-2790.
_||_Arts, M., Sebastiaan, J., Voss, H. P., Haenen, G. & Bast, A. (2004). A new approach to assess the total antioxidant capacity using the TEAC assay. Food Chemistry, 88(4), 567-570.
Azizi, F., Saadat, N., Salehi, P. & Emami, H.
(2001). The relationship between glucose intolerance and blood pressure, body mass index and waist to hip ratio in Tehran urban population (Tehran Lipid and Glucose Study). Iranian Journal of Endocrinology and Metabolism, 3(4), 256-247 [In Persian].
Bedford, T.G. (1979). Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology, 47(6), 1278-1283.
Bobik, A. (2005). The structural Basis of hypertwnsion vascular remodelling, rare faction and angiogenesis /arteriogenesis. Journal of Hypertension, 23,1473–1475.
Erekat, N. S., Al-Jarrah, M. D. & Khatib, A. J. (2017). Treadmill exercise training improves vascular growth factor expression in the cardiac muscle of TypeI diabetic rats. Cardiol.
Gavin, T., Drew, J., Kubik, C., Pofahl, W. & Hickner, R. (2007). Acute resistance exercise increases skeletal muscle angiogenic growth factor expression. Acta Physiology, (2)191, 46-139.
Goktepe, M. & Gunay, M. (2014). The effect of quercetin administration on exercise, Free Radical and Antioxidant Enzym Levels; 2 (5), 775-788.
Gordaliza, M. (2007). Natural products as leads to anticancer drugs. Clin Transl Oncol 9: 767-776.
Leandro, C. J. (2007). A program of moderate physical training for Wistar rats based on maximal oxygen consumption. Journal of Strength and Conditioning Research, 21(3), 751.
Lee, S. (2004). Viral expression of insulin- like growth factor-l enhances muscle, hypertrophy in resistance – training rats. Journal of Applied Physiology, 96(3), 1097-1104.
Mahrou, M., Gaeini, A., Chobbineh, S. & Javidi, M. (2014). Changes in simulating factors of angiogenesis, induced by progressive resistance training diabetic rats. Iranian journal of Diabetes and Metabolism, 14(1), 2-8 [In Persian].
Molla Hasanzadeh, F., Bijeh, N. & Moazami, M. (2017). The effect of eight weeks of aerobic exercise on angiogenesis and body composition in overweight women. Sport physiology, 8(29), 56-32 [In Persian].
Moradi, E., Heydarian, E., Gholami, M., Saffari, J., Mardani, G., Karimi, A. &Nourmohammadi, Z. (2015). Effect of Quercetin on Methotrexate-induced Hepatic and Renal Damages in Male Rats. Journal Mazandaran University Medical Science, 25 (127), 25-37 [In Persian].
Noorshahi, M., Emamian Rostami, M. & Khodagholi, F. (2017). The effect of 8 weeks of intermittent speed training on VEGF levels in skeletal muscle tissue of elderly rats. Medical Journal of Tabriz University of Medical Sciences and Health Services, 40 (2), 94-88 [In Persian].
Nourshahi, M., Farahmand, F. & Bigdeli, M. R. (2017). Effect of cinnamon-extract supplementation on VEGF and Endostatin level in hind leg muscle of aged rats after one session of exhaustive exercise. Iran Journal of Physiology Pharmacology., 1(3), 176-184 [In Persian].
Paczek, B., Bartlomiejczyk, I. & Przybylski, J. (2006). The serum levels of growth factors: PDGF, TGF-BET and VEGF are induced after strenuous physical exercise. Journal of Physiology Pharmacology, 191-197.
Roskoski, R. (2008). VEGF receptor protein–tyrosine kinases: Structure and regulation. Biochemical and Biophysical Research Communications, 3, 287–291.
Soori, R., Sharafi Dehrahm, F., Choobineh, S., Valipour Dehnou, V. (2018). Effect of endurance training on VEGF protein level in tissue of cardiac muscle in STZ-induced diabetic Wistar rats. Journal of Medical Sciences of Lorestan University (Yafteh), 20(3), 110-124 [In Persian].
Taheri Chadernshin, H., Noorshahi, M., Ranjbar, K. (2010). The response of vascular endothelial growth factor to exhausted submaximal exercise and its relationship with VO2max. Journal of Sport Biosciences, 7, 75-59 [In Persian].
Tang, K., Xia, F. C., WagneSr, P. D. & Breen, E.C. (2010). Exercise – induced VEGF transcriptional activitation in brain, lung and skeletal muscle. Respiratory Physiology & Neurobiology, 170, 16-22.
Tiago, F., Baraúna, V. G., Negrão, C. E., Phillips, M. I. & Oliveira, E. M. (2015). Aerobic exercise training, 80(3), 35-50.
Urso, M. L. & Clarkson, P. M. (2003). Oxidative stress, exercise and antioxidant supplementation. Toxicol, 189 (1-2), 41 - 54.
Ushio Fukai, M. & Yoshimasa, N. (2008). Reactive oxygen species and angiogenesis: NADPH oxidase as target for cancer therapy. Cancer Lett, 266, 37–52.
Uysal, N. (2015). Effects of voluntary and involuntary exercise on cognitive functions, and VEGF and BDNF levels in adolescent rats. Biotechnic & Histochemistry, 90 (1), 55-68.
Vali Zadeh, S., Motamedi, P., Karami, H. & Hamid, Rajabi. (2018). The Effects of Endurance Training on Gene Expression of VEGF and VEGFR2 of Cardiac Tissue in Type 2 Diabetic Male Wistar. Journal of Arak University of Medical Sciences, 21(6), 108-117 [In Persian].
Werner, G. S., Ferrari, M., Betge, S., Gastmann, O., Richartz, B. M. & Figulla, H. R. (2001). Collateral function in chronic total coronary occlusions is related to regional myocardial function and duration of occlusion. Circulation, 104(23), 2784-2790.
