The Effect of Ultrasonic Pretreatment on Antioxidant Activity and Phenolic Compounds of White and Brown Rice Extracted from Lenjan Sorkhe Cultivar

Subject Areas : Microbiology

K.  Pourmohammadi ¹ (Assistant professor of the Department of Food Science and Technology, Fasa University, Fasa, Iran.)
E.  Abedi ² (Assistant professor of the Department of Food Science and Technology, Fasa University, Fasa, Iran.)
M.  Khaleghan ³ (M.Sc Student of the Department of Food Science and Technology, Shahreza Branch, Islamic Azad University, Esfahan, Iran.)
M.R.  Mahmoodi ⁴ (Assistant Professor of the Department of Statistic, Fasa University, Fasa, Iran.)

Received: 2019-12-26 Accepted : 2019-12-26 Published : 2019-12-22

Keywords: Brown Rice, Phenolic Compounds, Ultrasonic Pretreatment, White Rice,

Abstract :

Introduction: Brown rice cultivars has higher nutritional values than the white rice cultivars due to the total phenolic compounds. Ultrasonic pretreatment is an efficient method for the extraction of phenolic compounds due to time saving, high performance efficiency and also less solvent consumption. Materials and Methods: Total phenolic compounds and flavonoid contents were determined and antioxidant potential was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Bioactive compounds of white and brown rice were extracted under various solvents including (water, ethanol 100%, methanol 100%, ethanol 50%, methanol 50% and ethanol 50%-methanol 50%) and two extraction methods such as Soxhlet extractor and ultrasonic bath. The comparison of the test variables were performed by Duncan's test and three-way multivariate analysis of variance. Results: The results showed that the highest flavonoids content (162 mg gallic acid/ Kg sample), poly phenols (142.5 mg quercetin / kg sample) and radicals scavenging effect DPPH (%76.4) belonged to brown rice under ultrasonic method and ethanol:methanol (50:50) solvent extraction. The results exibited that all the main related factors such as methods of extraction, solvents and rice have significant effects on the amount of polyphenols, flavonoids and DPPH. Conclusion: Under the same extraction condition, total phenol and flavonoid contents of brown rice is higher than the white rice. Ultrasonic extraction could enhance the total phenol and flavonoids contents compared to the soxhlet extraction.

References:

Abozed, S. S., El-kalyoubi, M., Abdelrashid, A. & Salama, M. F. (2014). Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Annals of Agricultural Science, 59, 63–67.

Ahmadi Kamazani, N., Elhami Rad, A. H., Ghavami, M., Moridi Farimani, M. & Armin, M. (2017). Extraction of antioxidant extracts from lettuce waste with ultrasound Evaluation of its antioxidant activity. Food Technology & Nutrition, 14, 1-18.

Basiri, S. H., Shahidi, F., Kadkhodaee, R. & Farhosh, R. (2011). An investigation on the effect of ultrasound waves and pretreatment methods on the extraction of oil from pomegranate seeds. Journal of Food Science and Technology, 8, 115-122.

Chandrapala, J., Oliver, C., Kentishc, S. & Ashokkumar, M. (2012a). Ultrasonics in food

 

processing, Food quality assurance and food safety. Trends in Food Science & Technology, 26, 88-98.

Chandrapala, J., Oliver, C., Kentishc, S. & Ashokkumar, M. (2012b). Ultrasonics in food processing. Ultrasonics Sonochemistry, 19, 975–983.

Do, Q., Angkawijaya, D., A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S. & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22, 296 -302.

Fereidoni Nory, T., Fahimdanesh, M. & Sahari, M. A. (2016). Investigation extraction of rosemary leaves the phenolic compounds by ultrasonic technique and its effect on organoleptic properties, physicochemical and stability of virgin olive oil. Journal of Food Science and Technology, 53, 112-125.

Ghaderi Ghahfarokhi, M., Alami, M., Sadeghi Mahoonak, A., Ghorbani, M. & Azizi, M. (2011). Chemical composition and effect of thermal processing methods on polyphenol content of two Iranian acorn varieties.  Journal of Iranian Food Science Researches, 21, 421-431.

Jung, C. H., Seog, H. M., Choi, I. W., Park, M. W. & Cho, H. Y. (2006). Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT Food Science and Technology, 39, 266-274.

Karami, Z., Emam Jomeh, Z., Mirzaei, H., Sadeghi Mahonak, A., Khomeiri, M. & Aydani, E. (2011). Investigation and comparison of Ultrasonic Assisted Extraction (UAE) and Soxhlet Extraction of phenolic compound from licorice root. Food Processing and Storage, 2, 1-22.

Kentish, S. & Ashokkumar, M. (2011). The Physical and Chemical Effects of Ultrasound, in Ultrasound technologies for food and bioprocessing, edited by Feng, H., Barbosa, G. V. & Weiss, J. Springer, New York, pp. 235-252.

Kermani, A. M., Tavakoli Hashjin, T.&  Minaei, S. (2008). Investigation and Determination of Moisture Content and Temperature Effects on the Mechanical Properties of Brown Rice. Journal of Agricultural Engineering Research, 9, 53-74.

Khajeh Nori, M. & Haghighi Asl, E. (2014). Investigation of extraction of natural compounds of plants using microwave and ultrasonic waves. Journal of Food Science and Modern Innovation, 3, 81-91.

Kumar, S. & Pandey, A. K. (2013). Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal, 2013, 1-16.

Mardani Ghahfarokhi, V., Alami, M., Arabshahi Deloui, S., Khodabakhshi, R. & Ghaderi Ghahfarokhi, M. (2013). Evaluation of antioxidant