Comparison of the antioxidant effect of vitamin E and Curcuma Longa L. extract on the histopathological lesions of Liver and kidney following aspartame consumption in rat

Subject Areas : Journal of Comparative Pathobiology

Ghavidel, S., Davari, S.A.*, Rasekh, M. . ¹ ( . )

Received: 2018-04-13 Accepted : 2018-04-13 Published : 2018-06-22

Keywords: antioxidant, Vitamin E, Curcum Longa L, Aspartame, Liver, Kidney,

Abstract :

Aspartame is one of the most widely used artificial sweeteners in worldwide. The aim of this study was comparison of the antioxidant effect of vitamin E and Curcuma Longa L. extract on the histopathological lesions of aspartame consumption in rat. 32 males adult Wistar rats were randomly divided into four equal groups as follows: (1) control, (2) receiving aspartame (350 mg/kg) orally, (3) receiving aspartame (350 mg/kg) orally and vitamin E (400 mg/kg) by peritoneal injection, (4) receiving aspartame (350 mg/kg) and Curcuma Longa L. extract (200 mg/kg) orally for 1 month. At the end of the experiment, after tissue processing of livers and kidneys of rats, histopathological changes were examined and analyzed by using the Kruskal-Wallis test. Histopathological examination of liver in group 2 showed disruption of the hepatic cords, vacuolar degeneration and accumulation of necrotic materials in hepatocytes. Furthermore, renal tissue evaluation revealed degeneration of proximal and distal convoluted tubules; glomerular atrophy and increasing of urinary space. Above-mentioned lesions were significantly improved in groups 3 and 4 in comparison to group 2 (P<0.001). Besides, Vitamin E and Curcuma Longa L. extract had higher therapeutic effects in the liver tissue in comparison to the kidney. Furthermore, treatment with vitamin E caused a significant decrease of histopathological lesions in comparison to Curcuma Longa L. extract (P<0.001). These results showed the antioxidant effects of vitamin E and Curcuma Longa L. extract on the lesions of aspartame consumption and this protection effect was more pronounced in the group received vitamin E.

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