Comparison of laboratory and semi-industrial extraction methods for β-glucan-rich polysaccharide production from Shiitake mushroom (Lentinula edodes)
Subject Areas : food biotechnology
sharareh rezaeian
1
,
Hamidreza Pourianfar
2
1 - عضو هیات علمی سازمان جهاد دانشگاهی خراسان رضوی
2 - Industrial Fungi Biotechnology Research Department, Academic Center for Education, Culture and Research (ACECR)- Khorasan Razavi Branch, Mashhad, Iran
Keywords: Shiitake, β-glucan, polysaccharides, extraction, semi-industrial scale, nutrition, safety,
Abstract :
Shiitake mushroom (Lentinula edodes) is recognized as a potent source of health-promoting polysaccharides, particularly β-glucans. This study established a semi-industrial protocol for producing β-glucan-enriched polysaccharide extracts from dried shiitake fruiting bodies. A multi-step procedure involving hot-water extraction, ethanol precipitation, and spray drying was implemented at both laboratory and semi-industrial scales. The extraction yield reached 33.00% at the laboratory scale and 25.00% at the semi-industrial scale. Enzymatic quantification indicated β-glucan and α-glucan contents of approximately 28.00% and 5.00%, respectively, with no significant variation between scales (p ≥ 0.05). The final extract demonstrated acceptable microbial quality, an absence of toxic metals and pathogens, and favorable nutritional characteristics. Compared to a commercial reference product available in the Iranian market, the extract exhibited higher protein and fiber contents, as well as more than double the β-glucan concentration (28.00% vs. 13.6%). The method developed in the present study offers a scalable, reproducible, and economically viable approach for the production of high-purity shiitake polysaccharide extracts suitable for functional food and nutraceutical applications.
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