Investigating the effect of eugenol on the pancreas of Wistar rats with polycystic ovary syndrome induced by estradiol valerate

Subject Areas : Journal of Comparative Pathobiology

Zahra Kokabiyan ¹ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
زهرا Hajebrahimi ² (عضو هییت علمی پژوهشگاه هوا و فضا)
پریچهره Yaghmaei ³ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran )
S.B Jameie ⁴ (مرکز تحقیقات علوم اعصاب، دانشکده پیراپزشکی، دانشگاهعلوم پزشکی ایران)

Received: 2023-09-17 Accepted : 2023-11-11 Published : 2024-03-05

Keywords: Polycystic ovarian syndrome, Eugenol, Pancreas, Rat ,

Abstract :

Polycystic ovary syndrome (PCOS) is the most common endocrine disease in women of reproductive age. The cause of PCOS is still unknown, however, studies have shown that insulin resistance plays a role in the pathogenesis of this syndrome. In addition to insulin resistance, it has been shown that some women with PCOS have changes in the function of beta cells. Eugenol is a substance with anti-inflammatory and antioxidant properties. The aim of this study was to investigate the therapeutic effect of eugenol on pancreatic tissue in female Wistar rats with PCOS. In this experimental study, 30 Wistar rats weighing 200±20 grams were used. To induce polycystic ovary syndrome, estradiol valerate at a dose of 4 mg/kg was injected intraperitoneally for 28 days. Animals were divided into 5 groups. The control group and PCOS received normal food, the sham group received solvant, and two treatment groups with intraperitoneal injection of eugenol with doses of 12 and 24 mg/kg. Then, the pancreas of the animals was examined histologically after being fixed, prepared and stained by the H&E method. Pancreas tissue studies showed an increase in inflammatory cells, an increase in necrotic cells and an increase in destroyed islets in the PCOS group. Administration of eugenol improved the histological characteristics of the pancreas. Treatment with eugenol with doses of 12 or 24 mg/kg significantly reduced the number of inflammatory cells, necrotic cells and destroyed islets (p≤0.05). The findings showed that eugenol can be effective in improving PCOS disease and reducing its harmful effects.

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