Effect of different levels of Apple cider vinegar on liver enzymes and intestinal histopathology of broiler chickens
Subject Areas : Veterinary Clinical Pathologyhosniyeh heydari 1 , Mohammad Jahantigh 2 , سیده آیدا داوری 3 , Dariush Saadati 4
1 - D.V.M. Graduate, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran.
2 - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran.
3 - Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran.
4 - Associate Professor of Food Health Department, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran.
Keywords: Apple Cider Vinegar, Broilers, Intestinal Histopathology, Liver Enzymes. ,
Abstract :
Nowadays, to increase the food efficiency in the poultry industry, additives such as vinegar are used in the diet. The purpose of this research was to investigate the effect of different levels of apple vinegar in the diet of broiler chickens on the activity of liver enzymes and intestinal histopathology. For this purpose, 64 pieces of Ross 308 broiler chickens were randomly divided into 4 experimental groups. The diet in groups 2, 3, and 4 was mixed with 1, 2, and 3 percent apple vinegar, respectively, and group 1 was fed with normal diet. On the 14th and 28th days, one chick was randomly selected from each replication. Blood samples were collected from the brachial vein. Then, the activity of liver enzymes was checked using an Auto Analyzer and a dedicated kit. To observe intestinal histopathological changes, tissue samples were prepared from the jejunum. According to the results, the amount of Alanine aminotransferase (ALT) enzyme in groups 3 and 4 was significantly lower than the control group (p<0.05). However, there were no significant changes in the amount of Alkaline phosphatase (ALP) and Aspartate aminotransferase (AST). The results of histopathology of the jejunum showed an increase in the height of intestinal villi, the depth of Lieberkohn's crypts and the number of intestinal villi in the groups receiving apple vinegar. Based on our findings, it can be concluded that apple vinegar reduces the activity of some liver enzymes, increases the surface of villi in the jejunum, and thus improves intestinal function.
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