Comparison of the effect of Chlorella vulgaris microalgae extract with sodium bentonite on performance, immune response and Blood parameters of broiler chickens infected with aflatoxin

Subject Areas : Journal of Animal Biology

Seyed Saman  Seif ¹ (Department of Animal Sciences, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran )
Amir  Fattah ² (Department of Animal Sciences, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran )
mohsen  Mohamadisaei ³ (Animal Science Research Department, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran)

Received: 2023-09-06 Accepted : 2023-10-05 Published : 2024-01-05

Keywords: Aflatoxin, bentonite, chicken, performance, Chlorella vulgaris,

Abstract :

This study was conducted to investigate the effect of Chlorella vulgaris microalgae extract with sodium bentonite on the performance, immune responses and blood parameters of broiler chickens fed with aflatoxin-contaminated diet. The experiment was conducted using 384 chicken pieces in a completely random design and were divided into 6 experimental groups in 4 repetitions and 16 pieces in each repetition. Experimental rations include: 1- negative control treatment (rations without aflatoxin); 2- Positive control treatment (rations contaminated with 0.5 mg/kg of aflatoxin); 3- Healthy diet + Chlorella vulgaris extract (0.4 g/kg feed); 4- Contaminated diet + chlorella extract (0.4 g/kg of feed); 5- Healthy diet + sodium bentonite (2 grams per kilogram of feed); 6- Contaminated diet + sodium bentonite (2 grams per kilogram of feed). In this study, functional parameters, hematological and immune parameters were determined. The results of the research showed that the addition of bentonite and chlorella food supplements to the basic diet in the final period did not affect weight gain and feed consumption, but in the initial period of growth and the whole period improved weight gain and feed consumption compared to the control group (p < 0.05). There was no statistically significant difference between the control treatment and the aflatoxin treatment in any of the experimental periods in the field of food conversion coefficient (p < 0.05). Chickens fed with 0.4 g/kg of chlorella had the best performance in terms of blood biochemical metabolites compared to other experimental treatments. Adding chlorella supplement to the food diet compared to the control diet showed a positive and significant effect on the antibody titer and the relative weight of the lymphatic organs (p < 0.05), but bentonite did not showe a significant difference with the control in the spleen, thymus, and bursa.

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