The Short-term Effects of Aerobic Exercise and High-Fat Diet and Curcumin Extract on the Expression of Adiponectin and Interleukin Genes in Six Muscle Tissues in Male Wistar Rats

Subject Areas : Journal of Animal Biology

Sedighe  Aghayan ¹ (Department of Physical Education and Sports Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran)
Tahereh  Bagherpoor ² (Department of Physical Education and Sports Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran)
Nematollah  Nemati ³ (Department of Physical Education and Sports Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran)

Received: 2020-07-23 Accepted : 2020-10-28 Published : 2023-11-22

Keywords: Curcumin, Aerobic exercise, Adiponectin, High-fat diet, interleukin,

Abstract :

Adiponectin is a peptide hormone that is secreted from adipose tissue in humans and is effective on fat and glucose metabolism. Interleukin-6 also has the main role in various types of host defense mechanisms, including immune reactions and acute phase reactions. Therefore, the aim of the present study was to compare the short-term effects of aerobic exercise and high-fat diet and curcumin extract on the expression of adiponectin and interleukin genes in six muscle tissues of male Wistar rats. For this purpose, 50 two-month-old male rats were prepared and divided into five control groups, high-fat diet, curcumin and high-fat diet, exercise and high-fat diet, curcumin + exercise + high-fat diet. Data analysis was compared using one-way analysis of variance. The mean of intra-group differences was also compared using the one-way statistical test. The results showed that the average expression of the adiponectin gene of the subjects showed a significant difference among the 5 groups. The results of Bonferroni's post hoc test also showed a significant difference between the control group and other groups. Also, the expression of interleukin 6 gene increased in two groups - high-fat diet and high-fat + curcumin diet group compared to the control group. But in two groups of high-fat diet + exercise and high-fat diet + exercise + curcumin group, there was a significant decrease.

References:

1. Baek K., Kang J., Lee J., Kim M., Baek J.H. 2018. The Time Point-Specific Effect of Beta-Adrenergic Blockade in Attenuating High Fat Diet-Induced Obesity and Bone Loss. Calcified Tissue International, 19:1-13.


2. Debidi Roshan V., Barzegarzadeh H. The effect of obesity and activity intensity on serum adiponectin response and some biochemical indices in young women, Olympic, 19(2):141-154.


3. Dey G., Bharti R., Dhanarajan G., Das S., Dey K.K., Kumar B.P. 2015. Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer. Scientific Reports, 5:10316.


4. Frayn K.N., Fielding B.A., Humphreys S.M., Coppack S.W. 1996. Nutritional influences on human adipose-tissue metabolism. Biochemical Society Transactions, 24(2):422-426.


5. Frayn K.N. 2002. Insulin resistance, impaired postprandial lipid metabolism and abdominal obesity. A deadly triad. Medical Principles and Practice, 2:31-40.


6. Furukawa S., Fujita T., Shimabukuro M., Iwaki M., Yamada Y., Nakajima Y. 2017. Increased oxidative stress in obesity and its impact on metabolic syndrome. The Journal of Clinical Investigation, 114(12):1752-1761.


7. Huang W.C., Chiu W.C., Chuang H.L., Tang D.W., Lee Z.M., Wei L. 2015. Effect of curcumin supplementation on physiological fatigue and physical performance in mice. Nutrients, 7(2): 905-921.


8. Jack B.U., Malherbe C.J., Mamushi M.P., Muller C.J., Joubert E., Louw J., Pheiffer C. 2019. Adipose tissue as a possible therapeutic target for polyphenols: A case for Cyclopia extracts as anti-obesity nutraceuticals. Biomedicine and Pharmacotherapy, 120:109439.


9. Kandula V., Kosuru R., Li H., Yan D., Zhu Q., Lian Q. 2016. Forkhead box transcription factor 1: role in the pathogenesis of diabetic cardiomyopathy. Cardiovascular
   Diabetology, 15(1):44.


10. Kim J.H., Bachmann R.A., Chen J. 2009. Interleukin-6x and insulin resistance. Vitamins and Hormones, 80: 613-33.


11. Lundman P, Boquist S, Samnegård A, Bennermo M, Held C, Ericsson CG, Silveira A, Hamsten A, Tornvall P. 2007. A high-fat meal is accompanied by increased plasma interleukin-6 concentrations. Nutrition and Metabolism Cardiovascular Diseases, 17(3): 195-202.


12. Martins R., Lithgow G.J., Link W. 2016. Long live FOXO: unraveling the role of FOXO proteins in aging and longevity. Aging Cell, 15(2):196-207.


13. Milan G, Romanello V, Pescatore F, Armani A, Paik JH, Frasson L. 2015. Regulation of autophagy and the ubiquitin–proteasome system by the FoxO transcriptional network during muscle atrophy. Nature Communications, 6:6670.


14. Müller S., Martin S., Koenig W., Hanifi Moghaddam P., Rathmann W., Haastert B., Giani G., Illig T., Thorand B., Kolb H. 2002. Impaired glucose tolerance is associated with increased serum concentrations of interleukin 6 and co-regulated acute-phase proteins but not TNF-alpha or its receptors. Diabetologia, 45(6):805-812.


15. Palomer X., Álvarez-Guardia D., Rodríguez-Calvo R., Coll T., Laguna J.C., Davidson M.M. 2008. TNF-α reduces PGC-1 α expression through NF-κB and p38 MAPK leading to increased glucose oxidation in a human cardiac cell model. Cardiovascular Research, 4:703-12.


16. Park H.S., Park J.Y., YU R. 2005. Relationship of obesity and visceral adiposity with serum concentrations of CRP, and IL-6. Diabe Res Clin pract; 69:29-


17. Perl A. 2015. mTOR activation is a biomarker and a central pathway to autoimmune disorders, cancer, obesity, and aging. Annals of the New York Academy of Sciences, 1346(1):33-44.


18. Raoufi A., Sirous F., Hoseini A. 2019. The Effect of eight weeks of curcumin supplementation on the expression of some regulatory genes of atrophic processes in the heart tissue of fatty adult fatty rats. Journal of Fasa University of Medical Sciences, 9(2):1425-1432.


19. Relling D.P., Esberg L.B., Fang C.X., Johnson W.T., Murphy E.J., Carlson E.C. 2006. High-fat diet-induced juvenile obesity leads to cardiomyocyte dysfunction and upregulation of Foxo3a transcription factor independent of lipotoxicity and apoptosis. Journal of Hypertension, 3:549-561.


20. Singh S.P., Bellner L., Vanella L., Cao J., Falck J.R., Kappas A., Abraham N.G. 2016. Downregulation of PGC-1α prevents the beneficial effect of EET-Heme oxygenase-1 on mitochondrial integrity and associated metabolic function in obese mice. Journal of Nutrition and Metabolism, 2016:9039754.


21. Wang S., Moustaid-Moussa N., Chen L., Mo H., Shastri A., Su R. 2014. Novel insights of dietary polyphenols and obesity. The Journal of Nutritional Biochemistry, 25(1):1-8.