Evaluation of SCAMP5 Gene Expression and Its Target miR in the Tissue of Patients with Glioblastoma Multiforme

Subject Areas : Journal of Animal Biology

Nasrin Karimi ¹ , Hamidreza Kheiri Manjilli ² , Vajiheh Zarrinpour ³ , Mohammad mahdi Forghanifard ⁴

1 - Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
2 - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
3 - Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
4 - 1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran

Received: 2021-03-24 Accepted : 2021-05-14 Published : 2022-05-22

Keywords: Real-time PCR, biomarker, Glioblastoma multiforme, SCAMP5,

Abstract :

Glioblastoma multiforme is one of the most malignant and common brain tumors, and after current combination therapy, patient survival is relatively poor and the disease is always fatal. This study was aimed at investigating the expression of SCAMP5 as a possible biomarker in GBM and evaluating the target miR of this gene. 50 tissue samples with GBM form before treatment and 50 healthy tissue samples from the GBM tumor margin were collected as a control. RNA extraction and cDNA synthesis were performed for all samples. Subsequently, a specific primer was designed and the expression of SCAMP5 gene was examined using Real-time PCR. ACTB gene was used as the internal control. Statistical analysis was carried out using Graphpad Prism software version 8. Roc curve was used to evaluate the biomarker value of SCAMP5 gene. Decreased expression of SCAMP5 gene was observed in GBM samples and its expression was compared with the pathological characteristics of patients. Expression of SCAMP5 gene with patients’ age with P-value ˂0.05 was statistically significant. However, the expression of this gene with patients’ gender with P-value ˃ 0.05 was not statistically significant. SCAMP5 was expressed in tumor tissue of patients with GBM much lower than healthy tissue around the tumor. The results revealed that SCAMP5 expression decreased in patients older than 50 years. ROC curve showed that the SCAMP5 gene could have a biomarker value. According to this study, it may be possible to use the expression of this gene as a marker as an early diagnostic method.

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