Anti-Anxiety Effects of Four Weeks of Lemon Essential Oil Consumption with Swimming Practice in Adult Male Mice

Subject Areas : Journal of Animal Biology

Elham Esfandiyari Gharibvand ¹ , Abdolhassan Doulah ²

1 - Department of Physical Education and Sport Sciences, Ahvaz Branch, Islamic Azad University Ahvaz, Iran
2 - Department of Biology, Islamic Azad University, Ahvaz Branch, Ahvaz, Iran

Received: 2020-04-13 Accepted : 2020-08-20 Published : 2020-11-21

Keywords: Mouse, anxiety, Lemon, Swimming Practice,

Abstract :

Physical activity and herbs have been long used to treat psychological illnesses such as anxiety and stress. The present study was aimed at investigating the effect of four weeks of using lemon essential oil and swimming practice on the anxiety level in male mice. To this end, 40 male mice with a weight of 40 ± 5 g were studied. The mice were randomly assigned to four groups, including the control group, swimming practice, the lemon essential oil receiving group, and the swimming practice + lemon essential oil group. Swimming training consisted of four weeks of swimming in water for five 30-minutes sessions per week at a temperature of 25-27 ºC. Lemon oil (50 mg/kg) was administered by gavage. Anxiety behaviors were assessed by behavioral tests of elevated plus-maze and open field. The results of this study showed that prescribing a combination of lemon essential oil and exercise had a significant increase in the duration and the number of times in the margins and centrality (p < 0.05) and a significant decrease in the number of excretions compared to the control group (p < 0.001). The results showed that applying swimming practice alone and in combination with lemon essential oil reduces anxiety behaviors.

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