The Effects of Desmopressin Acetate during the Prenatal and Neonatal Development of the Hypothalamus-Pituitary-Gonadal Axis on Serum Levels of Sexual Hormones at Puberty in Male Rats
Subject Areas : Journal of Animal Biologyسید ابراهیم حسینی 1 , مختار مختاری 2 , گلشید ساکی 3
1 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه زیست شناسی، فارس، ایران.
2 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه زیست شناسی، فارس، ایران.
3 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه زیست شناسی، فارس، ایران.
Keywords: HPG, Desmopressin acetate, Neonatal, Prenatal, Sexual hormones,
Abstract :
Desmopressin acetate (DA) is one of the synthetic replacements of vasopressin. Considering the possible effects of this medication on the reproductive system, in the present study the effects of this medication during fetal and neonatal development of the hypothalamic pituitary gonadal (HPG) axis on sexual hormones of peripubertal male rat offspring was determined. In this experimental study fifty female and twenty adult male wistar rats were used. after becoming pregnant, females were divided into five groups of ten including the control group (no treatment), Sham group 1 and experimental group 1 which received intraperitoneal injection of distilled water and 6mcg/Kg/day DA, prenatally, and Sham group 2 and experimental group 2 that received nothing, but their offspring received intraperitoneal injection of distilled water and 6mcg/Kg/day DA, neonatally. At ninth weeks after birth, the concentration of sexual hormones was measured by Electrochemiluminescence. The results were statistically analyzed by one-way ANOVA test. The results indicate that the concentration of sexual hormones in prenatally and neonatally treated male rats was not significantly different than the control group. It is suggested that DA doesnandrsquo;t cross the placenta and doesnandrsquo;t interfere with fetal development of HPG axis of male rat offspring. Also, because of the stopped development of leydig cells and its inactivated receptors during neonatal period, the steroidogenesis of leydig cells during pripubertal period wouldnandrsquo;t undergo the effects of DA which was injected at the first week of life.
